Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS03335 and RALBFv3_RS03340 are separated by 96 nucleotides RALBFv3_RS03340 and RALBFv3_RS03345 are separated by 94 nucleotides RALBFv3_RS03345 and RALBFv3_RS03350 are separated by 11 nucleotides
RALBFv3_RS03335: RALBFv3_RS03335 - serine/threonine protein kinase, at 728,205 to 729,227
_RS03335
RALBFv3_RS03340: RALBFv3_RS03340 - serine/threonine-protein phosphatase, at 729,324 to 730,235
_RS03340
RALBFv3_RS03345: RALBFv3_RS03345 - ribonuclease PH, at 730,330 to 731,046
_RS03345
RALBFv3_RS03350: RALBFv3_RS03350 - non-canonical purine NTP pyrophosphatase, RdgB/HAM1 family, at 731,058 to 731,663
_RS03350
Position (kb) 729
730
731 Strain fitness (log2 ratio) -2
-1
0
1
2 at 728.457 kb on + strand, within RALBFv3_RS03335 at 728.458 kb on - strand, within RALBFv3_RS03335 at 728.548 kb on - strand, within RALBFv3_RS03335 at 728.548 kb on - strand, within RALBFv3_RS03335 at 728.548 kb on - strand, within RALBFv3_RS03335 at 728.554 kb on + strand, within RALBFv3_RS03335 at 728.554 kb on + strand, within RALBFv3_RS03335 at 728.729 kb on + strand, within RALBFv3_RS03335 at 728.733 kb on + strand, within RALBFv3_RS03335 at 728.734 kb on - strand, within RALBFv3_RS03335 at 728.824 kb on - strand, within RALBFv3_RS03335 at 728.824 kb on - strand, within RALBFv3_RS03335 at 728.892 kb on + strand, within RALBFv3_RS03335 at 728.892 kb on + strand, within RALBFv3_RS03335 at 728.892 kb on + strand, within RALBFv3_RS03335 at 728.893 kb on - strand, within RALBFv3_RS03335 at 728.913 kb on + strand, within RALBFv3_RS03335 at 728.913 kb on + strand, within RALBFv3_RS03335 at 728.913 kb on + strand, within RALBFv3_RS03335 at 728.914 kb on - strand, within RALBFv3_RS03335 at 728.914 kb on - strand, within RALBFv3_RS03335 at 728.914 kb on - strand, within RALBFv3_RS03335 at 729.006 kb on + strand, within RALBFv3_RS03335 at 729.006 kb on + strand, within RALBFv3_RS03335 at 729.007 kb on - strand, within RALBFv3_RS03335 at 729.007 kb on - strand, within RALBFv3_RS03335 at 729.225 kb on + strand at 729.225 kb on + strand at 729.225 kb on + strand at 729.244 kb on - strand at 729.585 kb on + strand, within RALBFv3_RS03340 at 729.813 kb on + strand, within RALBFv3_RS03340 at 730.517 kb on - strand, within RALBFv3_RS03345 at 730.741 kb on + strand, within RALBFv3_RS03345 at 730.858 kb on + strand, within RALBFv3_RS03345
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 728,457 + RALBFv3_RS03335 0.25 -0.1 728,458 - RALBFv3_RS03335 0.25 +0.3 728,548 - RALBFv3_RS03335 0.34 +2.1 728,548 - RALBFv3_RS03335 0.34 +0.3 728,548 - RALBFv3_RS03335 0.34 -0.1 728,554 + RALBFv3_RS03335 0.34 +0.4 728,554 + RALBFv3_RS03335 0.34 -0.2 728,729 + RALBFv3_RS03335 0.51 -0.2 728,733 + RALBFv3_RS03335 0.52 +0.1 728,734 - RALBFv3_RS03335 0.52 -1.3 728,824 - RALBFv3_RS03335 0.61 +1.1 728,824 - RALBFv3_RS03335 0.61 -0.3 728,892 + RALBFv3_RS03335 0.67 -0.1 728,892 + RALBFv3_RS03335 0.67 -0.0 728,892 + RALBFv3_RS03335 0.67 +0.9 728,893 - RALBFv3_RS03335 0.67 -0.2 728,913 + RALBFv3_RS03335 0.69 -0.1 728,913 + RALBFv3_RS03335 0.69 -0.3 728,913 + RALBFv3_RS03335 0.69 +0.4 728,914 - RALBFv3_RS03335 0.69 +0.5 728,914 - RALBFv3_RS03335 0.69 +0.5 728,914 - RALBFv3_RS03335 0.69 +0.3 729,006 + RALBFv3_RS03335 0.78 -0.1 729,006 + RALBFv3_RS03335 0.78 +0.2 729,007 - RALBFv3_RS03335 0.78 +0.3 729,007 - RALBFv3_RS03335 0.78 -0.5 729,225 + +0.3 729,225 + -0.5 729,225 + +0.0 729,244 - -0.4 729,585 + RALBFv3_RS03340 0.29 -1.1 729,813 + RALBFv3_RS03340 0.54 -1.9 730,517 - RALBFv3_RS03345 0.26 -0.3 730,741 + RALBFv3_RS03345 0.57 -0.1 730,858 + RALBFv3_RS03345 0.74 -0.1
Or see this region's nucleotide sequence