Strain Fitness in Ralstonia solanacearum IBSBF1503 around RALBFv3_RS03320

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRALBFv3_RS03315 and RALBFv3_RS03320 are separated by 234 nucleotidesRALBFv3_RS03320 and RALBFv3_RS03325 are separated by 131 nucleotidesRALBFv3_RS03325 and RALBFv3_RS03330 are separated by 17 nucleotides RALBFv3_RS03315: RALBFv3_RS03315 - bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase, at 723,445 to 725,871 _RS03315 RALBFv3_RS03320: RALBFv3_RS03320 - DNA-directed RNA polymerase subunit omega, at 726,106 to 726,309 _RS03320 RALBFv3_RS03325: RALBFv3_RS03325 - guanylate kinase, at 726,441 to 727,106 _RS03325 RALBFv3_RS03330: RALBFv3_RS03330 - YicC family protein, at 727,124 to 728,059 _RS03330 Position (kb) 726 727Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 726.004 kb on - strandat 726.097 kb on - strandat 726.097 kb on - strandat 726.237 kb on - strand, within RALBFv3_RS03320at 726.301 kb on - strandat 726.352 kb on - strandat 726.496 kb on + strandat 726.496 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
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726,004 - -0.9
726,097 - -0.4
726,097 - -1.8
726,237 - RALBFv3_RS03320 0.64 -4.8
726,301 - -0.9
726,352 - +1.1
726,496 + -0.9
726,496 + -0.5

Or see this region's nucleotide sequence