Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS03100 and RALBFv3_RS03105 are separated by 133 nucleotides RALBFv3_RS03105 and RALBFv3_RS03110 are separated by 169 nucleotides RALBFv3_RS03110 and RALBFv3_RS03115 are separated by 44 nucleotides RALBFv3_RS03115 and RALBFv3_RS03120 are separated by 23 nucleotides
RALBFv3_RS03100: RALBFv3_RS03100 - TIGR00730 family Rossman fold protein, at 678,687 to 679,271
_RS03100
RALBFv3_RS03105: RALBFv3_RS03105 - LysE family translocator, at 679,405 to 680,007
_RS03105
RALBFv3_RS03110: RALBFv3_RS03110 - DUF924 domain-containing protein, at 680,177 to 680,755
_RS03110
RALBFv3_RS03115: RALBFv3_RS03115 - oxygen-binding heme protein, at 680,800 to 681,204
_RS03115
RALBFv3_RS03120: RALBFv3_RS03120 - ABC transporter permease, at 681,228 to 683,759
_RS03120
Position (kb) 680
681 Strain fitness (log2 ratio) -1
0
1 at 679.556 kb on - strand, within RALBFv3_RS03105 at 679.652 kb on - strand, within RALBFv3_RS03105 at 679.734 kb on + strand, within RALBFv3_RS03105 at 679.735 kb on - strand, within RALBFv3_RS03105 at 679.783 kb on - strand, within RALBFv3_RS03105 at 679.783 kb on - strand, within RALBFv3_RS03105 at 679.819 kb on + strand, within RALBFv3_RS03105 at 679.819 kb on + strand, within RALBFv3_RS03105 at 679.820 kb on - strand, within RALBFv3_RS03105 at 679.820 kb on - strand, within RALBFv3_RS03105 at 679.926 kb on + strand, within RALBFv3_RS03105 at 679.987 kb on + strand at 679.988 kb on - strand at 680.404 kb on - strand, within RALBFv3_RS03110 at 680.993 kb on + strand, within RALBFv3_RS03115 at 680.994 kb on - strand, within RALBFv3_RS03115 at 681.116 kb on + strand, within RALBFv3_RS03115 at 681.116 kb on + strand, within RALBFv3_RS03115 at 681.204 kb on + strand at 681.311 kb on + strand at 681.458 kb on - strand at 681.742 kb on + strand, within RALBFv3_RS03120 at 681.742 kb on + strand, within RALBFv3_RS03120 at 681.742 kb on + strand, within RALBFv3_RS03120 at 681.742 kb on + strand, within RALBFv3_RS03120 at 681.743 kb on - strand, within RALBFv3_RS03120
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 679,556 - RALBFv3_RS03105 0.25 +0.0 679,652 - RALBFv3_RS03105 0.41 -0.8 679,734 + RALBFv3_RS03105 0.55 -0.4 679,735 - RALBFv3_RS03105 0.55 -0.1 679,783 - RALBFv3_RS03105 0.63 -0.3 679,783 - RALBFv3_RS03105 0.63 -0.4 679,819 + RALBFv3_RS03105 0.69 +0.1 679,819 + RALBFv3_RS03105 0.69 +0.8 679,820 - RALBFv3_RS03105 0.69 -0.6 679,820 - RALBFv3_RS03105 0.69 +0.6 679,926 + RALBFv3_RS03105 0.86 -0.9 679,987 + -0.7 679,988 - -0.2 680,404 - RALBFv3_RS03110 0.39 +0.0 680,993 + RALBFv3_RS03115 0.48 +0.5 680,994 - RALBFv3_RS03115 0.48 +0.1 681,116 + RALBFv3_RS03115 0.78 +0.1 681,116 + RALBFv3_RS03115 0.78 +0.2 681,204 + -0.3 681,311 + +0.7 681,458 - +1.0 681,742 + RALBFv3_RS03120 0.20 -0.5 681,742 + RALBFv3_RS03120 0.20 -0.1 681,742 + RALBFv3_RS03120 0.20 +0.1 681,742 + RALBFv3_RS03120 0.20 -0.6 681,743 - RALBFv3_RS03120 0.20 -0.3
Or see this region's nucleotide sequence