Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS03050 and RALBFv3_RS03055 are separated by 344 nucleotides RALBFv3_RS03055 and RALBFv3_RS03060 overlap by 4 nucleotides RALBFv3_RS03060 and RALBFv3_RS03065 are separated by 10 nucleotides
RALBFv3_RS03050: RALBFv3_RS03050 - acetolactate synthase 3 large subunit, at 669,452 to 671,209
_RS03050
RALBFv3_RS03055: RALBFv3_RS03055 - RNA polymerase sigma factor, at 671,554 to 672,120
_RS03055
RALBFv3_RS03060: RALBFv3_RS03060 - DUF3619 domain-containing protein, at 672,117 to 672,557
_RS03060
RALBFv3_RS03065: RALBFv3_RS03065 - DUF3106 domain-containing protein, at 672,568 to 673,425
_RS03065
Position (kb) 671
672
673 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 671.358 kb on + strand at 671.454 kb on + strand at 671.553 kb on + strand at 671.553 kb on + strand at 671.905 kb on + strand, within RALBFv3_RS03055 at 671.906 kb on - strand, within RALBFv3_RS03055 at 671.906 kb on - strand, within RALBFv3_RS03055 at 672.116 kb on + strand at 672.358 kb on + strand, within RALBFv3_RS03060 at 672.370 kb on + strand, within RALBFv3_RS03060 at 672.371 kb on - strand, within RALBFv3_RS03060 at 672.430 kb on - strand, within RALBFv3_RS03060 at 672.430 kb on - strand, within RALBFv3_RS03060 at 672.537 kb on + strand at 672.555 kb on + strand at 672.556 kb on - strand at 673.010 kb on - strand, within RALBFv3_RS03065 at 673.063 kb on + strand, within RALBFv3_RS03065 at 673.063 kb on + strand, within RALBFv3_RS03065 at 673.063 kb on + strand, within RALBFv3_RS03065 at 673.064 kb on - strand, within RALBFv3_RS03065
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 671,358 + +0.4 671,454 + +0.0 671,553 + -1.0 671,553 + -1.4 671,905 + RALBFv3_RS03055 0.62 -2.8 671,906 - RALBFv3_RS03055 0.62 -1.1 671,906 - RALBFv3_RS03055 0.62 -1.6 672,116 + +0.8 672,358 + RALBFv3_RS03060 0.55 +0.9 672,370 + RALBFv3_RS03060 0.57 -0.2 672,371 - RALBFv3_RS03060 0.58 +1.0 672,430 - RALBFv3_RS03060 0.71 -1.8 672,430 - RALBFv3_RS03060 0.71 +0.4 672,537 + +0.0 672,555 + +1.5 672,556 - +1.0 673,010 - RALBFv3_RS03065 0.52 +0.3 673,063 + RALBFv3_RS03065 0.58 -0.2 673,063 + RALBFv3_RS03065 0.58 +1.0 673,063 + RALBFv3_RS03065 0.58 +0.2 673,064 - RALBFv3_RS03065 0.58 +1.3
Or see this region's nucleotide sequence