Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS03000 and RALBFv3_RS03005 are separated by 91 nucleotides RALBFv3_RS03005 and RALBFv3_RS03010 are separated by 307 nucleotides RALBFv3_RS03010 and RALBFv3_RS03015 are separated by 162 nucleotides
RALBFv3_RS03000: RALBFv3_RS03000 - haloacid dehalogenase-like hydrolase, at 658,227 to 659,174
_RS03000
RALBFv3_RS03005: RALBFv3_RS03005 - polyribonucleotide nucleotidyltransferase, at 659,266 to 661,440
_RS03005
RALBFv3_RS03010: RALBFv3_RS03010 - 30S ribosomal protein S15, at 661,748 to 662,017
_RS03010
RALBFv3_RS03015: RALBFv3_RS03015 - branched-chain amino acid ABC transporter substrate-binding protein, at 662,180 to 663,436
_RS03015
Position (kb) 659
660
661
662 Strain fitness (log2 ratio) -1
0
1
2
3 at 658.459 kb on + strand, within RALBFv3_RS03000 at 658.468 kb on + strand, within RALBFv3_RS03000 at 658.469 kb on - strand, within RALBFv3_RS03000 at 658.473 kb on - strand, within RALBFv3_RS03000 at 658.656 kb on - strand, within RALBFv3_RS03000 at 658.669 kb on + strand, within RALBFv3_RS03000 at 658.670 kb on - strand, within RALBFv3_RS03000 at 658.670 kb on - strand, within RALBFv3_RS03000 at 658.670 kb on - strand, within RALBFv3_RS03000 at 658.748 kb on - strand, within RALBFv3_RS03000 at 658.801 kb on + strand, within RALBFv3_RS03000 at 658.801 kb on + strand, within RALBFv3_RS03000 at 658.802 kb on - strand, within RALBFv3_RS03000 at 658.818 kb on - strand, within RALBFv3_RS03000 at 658.832 kb on - strand, within RALBFv3_RS03000 at 659.104 kb on + strand at 659.104 kb on + strand at 659.210 kb on - strand at 659.567 kb on + strand, within RALBFv3_RS03005 at 659.568 kb on - strand, within RALBFv3_RS03005 at 661.754 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 658,459 + RALBFv3_RS03000 0.24 -1.0 658,468 + RALBFv3_RS03000 0.25 +0.2 658,469 - RALBFv3_RS03000 0.26 +0.3 658,473 - RALBFv3_RS03000 0.26 -1.0 658,656 - RALBFv3_RS03000 0.45 -0.6 658,669 + RALBFv3_RS03000 0.47 +0.3 658,670 - RALBFv3_RS03000 0.47 +1.0 658,670 - RALBFv3_RS03000 0.47 +1.0 658,670 - RALBFv3_RS03000 0.47 +0.1 658,748 - RALBFv3_RS03000 0.55 +1.3 658,801 + RALBFv3_RS03000 0.61 -0.2 658,801 + RALBFv3_RS03000 0.61 -0.4 658,802 - RALBFv3_RS03000 0.61 +0.1 658,818 - RALBFv3_RS03000 0.62 -1.1 658,832 - RALBFv3_RS03000 0.64 -0.1 659,104 + -0.8 659,104 + +0.7 659,210 - +3.0 659,567 + RALBFv3_RS03005 0.14 -0.8 659,568 - RALBFv3_RS03005 0.14 -0.6 661,754 - +0.6
Or see this region's nucleotide sequence