Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS02755 and RALBFv3_RS02760 are separated by 18 nucleotides RALBFv3_RS02760 and RALBFv3_RS02765 are separated by 359 nucleotides RALBFv3_RS02765 and RALBFv3_RS02770 overlap by 79 nucleotides RALBFv3_RS02770 and RALBFv3_RS02775 are separated by 38 nucleotides
RALBFv3_RS02755: RALBFv3_RS02755 - phosphotransferase family protein, at 613,229 to 614,314
_RS02755
RALBFv3_RS02760: RALBFv3_RS02760 - glyoxalase, at 614,333 to 614,743
_RS02760
RALBFv3_RS02765: RALBFv3_RS02765 - LysR family transcriptional regulator, at 615,103 to 616,077
_RS02765
RALBFv3_RS02770: RALBFv3_RS02770 - LysR family transcriptional regulator, at 615,999 to 616,970
_RS02770
RALBFv3_RS02775: RALBFv3_RS02775 - PaaI family thioesterase, at 617,009 to 617,509
_RS02775
Position (kb) 615
616
617 Strain fitness (log2 ratio) -2
-1
0
1 at 614.165 kb on + strand, within RALBFv3_RS02755 at 614.743 kb on + strand at 614.743 kb on + strand at 614.744 kb on - strand at 614.744 kb on - strand at 615.070 kb on - strand at 615.613 kb on + strand, within RALBFv3_RS02765 at 616.132 kb on + strand, within RALBFv3_RS02770 at 616.372 kb on + strand, within RALBFv3_RS02770 at 616.900 kb on + strand at 616.900 kb on + strand at 617.008 kb on + strand at 617.009 kb on - strand at 617.009 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 614,165 + RALBFv3_RS02755 0.86 -0.3 614,743 + -2.1 614,743 + -1.1 614,744 - +1.6 614,744 - -0.4 615,070 - +1.0 615,613 + RALBFv3_RS02765 0.52 -0.8 616,132 + RALBFv3_RS02770 0.14 +0.2 616,372 + RALBFv3_RS02770 0.38 +0.1 616,900 + -0.7 616,900 + +0.1 617,008 + +0.6 617,009 - -0.9 617,009 - -0.9
Or see this region's nucleotide sequence