Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS02715 and RALBFv3_RS02720 are separated by 32 nucleotides RALBFv3_RS02720 and RALBFv3_RS02725 are separated by 37 nucleotides RALBFv3_RS02725 and RALBFv3_RS02730 are separated by 35 nucleotides
RALBFv3_RS02715: RALBFv3_RS02715 - hypothetical protein, at 605,592 to 606,737
_RS02715
RALBFv3_RS02720: RALBFv3_RS02720 - peroxide stress protein YaaA, at 606,770 to 607,543
_RS02720
RALBFv3_RS02725: RALBFv3_RS02725 - putative toxin-antitoxin system toxin component, PIN family, at 607,581 to 608,066
_RS02725
RALBFv3_RS02730: RALBFv3_RS02730 - pyridoxal phosphate-dependent aminotransferase, at 608,102 to 609,319
_RS02730
Position (kb) 606
607
608 Strain fitness (log2 ratio) -2
-1
0
1
2 at 605.915 kb on - strand, within RALBFv3_RS02715 at 606.440 kb on + strand, within RALBFv3_RS02715 at 606.496 kb on + strand, within RALBFv3_RS02715 at 606.514 kb on + strand, within RALBFv3_RS02715 at 606.515 kb on - strand, within RALBFv3_RS02715 at 606.515 kb on - strand, within RALBFv3_RS02715 at 606.515 kb on - strand, within RALBFv3_RS02715 at 606.536 kb on - strand, within RALBFv3_RS02715 at 607.354 kb on - strand, within RALBFv3_RS02720 at 607.478 kb on - strand at 607.478 kb on - strand at 607.507 kb on - strand at 607.576 kb on - strand at 607.628 kb on + strand at 607.773 kb on + strand, within RALBFv3_RS02725 at 607.938 kb on + strand, within RALBFv3_RS02725 at 607.992 kb on + strand, within RALBFv3_RS02725 at 607.993 kb on - strand, within RALBFv3_RS02725 at 608.064 kb on + strand at 608.412 kb on - strand, within RALBFv3_RS02730 at 608.423 kb on + strand, within RALBFv3_RS02730 at 608.535 kb on - strand, within RALBFv3_RS02730
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 605,915 - RALBFv3_RS02715 0.28 +0.3 606,440 + RALBFv3_RS02715 0.74 -2.0 606,496 + RALBFv3_RS02715 0.79 -0.3 606,514 + RALBFv3_RS02715 0.80 +2.0 606,515 - RALBFv3_RS02715 0.81 +0.2 606,515 - RALBFv3_RS02715 0.81 -0.1 606,515 - RALBFv3_RS02715 0.81 -1.3 606,536 - RALBFv3_RS02715 0.82 -0.2 607,354 - RALBFv3_RS02720 0.75 -0.7 607,478 - +0.0 607,478 - -0.2 607,507 - -0.5 607,576 - +0.2 607,628 + -0.1 607,773 + RALBFv3_RS02725 0.40 +0.2 607,938 + RALBFv3_RS02725 0.73 +0.1 607,992 + RALBFv3_RS02725 0.85 -0.1 607,993 - RALBFv3_RS02725 0.85 +0.1 608,064 + +0.0 608,412 - RALBFv3_RS02730 0.25 -0.5 608,423 + RALBFv3_RS02730 0.26 +0.0 608,535 - RALBFv3_RS02730 0.36 +0.3
Or see this region's nucleotide sequence