Strain Fitness in Ralstonia solanacearum IBSBF1503 around RALBFv3_RS02670

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRALBFv3_RS02665 and RALBFv3_RS02670 are separated by 31 nucleotidesRALBFv3_RS02670 and RALBFv3_RS02675 are separated by 29 nucleotides RALBFv3_RS02665: RALBFv3_RS02665 - CoA ester lyase, at 593,020 to 594,000 _RS02665 RALBFv3_RS02670: RALBFv3_RS02670 - methylisocitrate lyase, at 594,032 to 594,928 _RS02670 RALBFv3_RS02675: RALBFv3_RS02675 - 2-methylcitrate synthase, at 594,958 to 596,115 _RS02675 Position (kb) 594 595Strain fitness (log2 ratio) -1 0 1at 593.345 kb on - strand, within RALBFv3_RS02665at 593.915 kb on - strandat 594.168 kb on - strand, within RALBFv3_RS02670at 594.379 kb on + strand, within RALBFv3_RS02670

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
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593,345 - RALBFv3_RS02665 0.33 -1.4
593,915 - -0.9
594,168 - RALBFv3_RS02670 0.15 -1.0
594,379 + RALBFv3_RS02670 0.39 -0.1

Or see this region's nucleotide sequence