Strain Fitness in Ralstonia solanacearum IBSBF1503 around RALBFv3_RS02610

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRALBFv3_RS02605 and RALBFv3_RS02610 are separated by 332 nucleotidesRALBFv3_RS02610 and RALBFv3_RS02615 are separated by 31 nucleotidesRALBFv3_RS02615 and RALBFv3_RS02620 are separated by 19 nucleotides RALBFv3_RS02605: RALBFv3_RS02605 - aspartate-semialdehyde dehydrogenase, at 580,628 to 581,731 _RS02605 RALBFv3_RS02610: RALBFv3_RS02610 - 3-isopropylmalate dehydrogenase, at 582,064 to 583,134 _RS02610 RALBFv3_RS02615: RALBFv3_RS02615 - 3-isopropylmalate dehydratase small subunit, at 583,166 to 583,816 _RS02615 RALBFv3_RS02620: RALBFv3_RS02620 - 3-isopropylmalate dehydratase large subunit, at 583,836 to 585,245 _RS02620 Position (kb) 582 583 584Strain fitness (log2 ratio) -1 0 1at 582.216 kb on - strand, within RALBFv3_RS02610at 583.149 kb on + strandat 583.149 kb on + strandat 583.287 kb on + strand, within RALBFv3_RS02615at 583.288 kb on - strand, within RALBFv3_RS02615at 583.674 kb on + strand, within RALBFv3_RS02615at 583.675 kb on - strand, within RALBFv3_RS02615

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
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582,216 - RALBFv3_RS02610 0.14 -0.8
583,149 + +0.6
583,149 + +0.3
583,287 + RALBFv3_RS02615 0.19 +0.0
583,288 - RALBFv3_RS02615 0.19 -0.5
583,674 + RALBFv3_RS02615 0.78 -0.5
583,675 - RALBFv3_RS02615 0.78 +0.0

Or see this region's nucleotide sequence