Strain Fitness in Ralstonia solanacearum IBSBF1503 around RALBFv3_RS02165

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRALBFv3_RS02160 and RALBFv3_RS02165 are separated by 197 nucleotidesRALBFv3_RS02165 and RALBFv3_RS02170 are separated by 134 nucleotidesRALBFv3_RS02170 and RALBFv3_RS02175 are separated by 94 nucleotides RALBFv3_RS02160: RALBFv3_RS02160 - dihydropteroate synthase, at 482,227 to 483,024 _RS02160 RALBFv3_RS02165: RALBFv3_RS02165 - ATP-dependent zinc metalloprotease FtsH, at 483,222 to 485,108 _RS02165 RALBFv3_RS02170: RALBFv3_RS02170 - RlmE family RNA methyltransferase, at 485,243 to 485,908 _RS02170 RALBFv3_RS02175: RALBFv3_RS02175 - RNA-binding protein, at 486,003 to 486,500 _RS02175 Position (kb) 483 484 485 486Strain fitness (log2 ratio) -3 -2 -1 0 1at 483.105 kb on + strandat 483.106 kb on - strandat 484.751 kb on - strand, within RALBFv3_RS02165at 484.853 kb on - strand, within RALBFv3_RS02165at 485.000 kb on - strandat 485.123 kb on - strandat 485.154 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
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483,105 + +0.0
483,106 - -1.3
484,751 - RALBFv3_RS02165 0.81 -2.9
484,853 - RALBFv3_RS02165 0.86 -2.3
485,000 - -3.2
485,123 - -0.5
485,154 - +0.2

Or see this region's nucleotide sequence