Strain Fitness in Ralstonia solanacearum IBSBF1503 around RALBFv3_RS01490

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRALBFv3_RS01485 and RALBFv3_RS01490 are separated by 230 nucleotidesRALBFv3_RS01490 and RALBFv3_RS01495 are separated by 385 nucleotides RALBFv3_RS01485: RALBFv3_RS01485 - dihydrouridine synthase, at 334,742 to 335,722 _RS01485 RALBFv3_RS01490: RALBFv3_RS01490 - polyhydroxyalkanoate synthesis repressor PhaR, at 335,953 to 336,510 _RS01490 RALBFv3_RS01495: RALBFv3_RS01495 - beta-ketoacyl-ACP reductase, at 336,896 to 337,636 _RS01495 Position (kb) 335 336 337Strain fitness (log2 ratio) -1 0 1at 335.584 kb on - strand, within RALBFv3_RS01485at 336.066 kb on - strand, within RALBFv3_RS01490at 336.218 kb on + strand, within RALBFv3_RS01490at 336.218 kb on + strand, within RALBFv3_RS01490at 336.219 kb on - strand, within RALBFv3_RS01490at 336.252 kb on - strand, within RALBFv3_RS01490at 336.429 kb on - strand, within RALBFv3_RS01490at 336.449 kb on + strand, within RALBFv3_RS01490at 337.179 kb on + strand, within RALBFv3_RS01495at 337.375 kb on + strand, within RALBFv3_RS01495

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
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335,584 - RALBFv3_RS01485 0.86 -1.5
336,066 - RALBFv3_RS01490 0.20 +0.5
336,218 + RALBFv3_RS01490 0.47 +0.7
336,218 + RALBFv3_RS01490 0.47 +0.9
336,219 - RALBFv3_RS01490 0.48 +0.7
336,252 - RALBFv3_RS01490 0.54 +0.6
336,429 - RALBFv3_RS01490 0.85 -0.2
336,449 + RALBFv3_RS01490 0.89 +1.0
337,179 + RALBFv3_RS01495 0.38 +0.1
337,375 + RALBFv3_RS01495 0.65 -0.2

Or see this region's nucleotide sequence