Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS01435 and RALBFv3_RS01440 overlap by 4 nucleotides RALBFv3_RS01440 and RALBFv3_RS01445 are separated by 175 nucleotides RALBFv3_RS01445 and RALBFv3_RS01450 are separated by 36 nucleotides
RALBFv3_RS01435: RALBFv3_RS01435 - 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase, at 321,786 to 322,292
_RS01435
RALBFv3_RS01440: RALBFv3_RS01440 - 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase, at 322,289 to 323,050
_RS01440
RALBFv3_RS01445: RALBFv3_RS01445 - transcription-repair coupling factor, at 323,226 to 326,669
_RS01445
RALBFv3_RS01450: RALBFv3_RS01450 - acetylornithine deacetylase, at 326,706 to 327,899
_RS01450
Position (kb) 323
324
325
326
327 Strain fitness (log2 ratio) -2
-1
0
1 at 323.130 kb on - strand at 323.249 kb on + strand at 323.249 kb on + strand at 323.628 kb on + strand, within RALBFv3_RS01445 at 323.986 kb on - strand, within RALBFv3_RS01445 at 323.986 kb on - strand, within RALBFv3_RS01445 at 324.028 kb on - strand, within RALBFv3_RS01445 at 324.211 kb on - strand, within RALBFv3_RS01445 at 324.528 kb on + strand, within RALBFv3_RS01445 at 324.777 kb on + strand, within RALBFv3_RS01445 at 324.798 kb on + strand, within RALBFv3_RS01445 at 324.798 kb on + strand, within RALBFv3_RS01445 at 324.799 kb on - strand, within RALBFv3_RS01445 at 324.803 kb on - strand, within RALBFv3_RS01445 at 324.803 kb on - strand, within RALBFv3_RS01445 at 324.803 kb on - strand, within RALBFv3_RS01445 at 324.955 kb on - strand, within RALBFv3_RS01445 at 325.972 kb on - strand, within RALBFv3_RS01445 at 325.978 kb on - strand, within RALBFv3_RS01445 at 326.071 kb on - strand, within RALBFv3_RS01445 at 326.163 kb on + strand, within RALBFv3_RS01445 at 326.848 kb on + strand, within RALBFv3_RS01450 at 326.848 kb on + strand, within RALBFv3_RS01450 at 326.848 kb on + strand, within RALBFv3_RS01450 at 326.848 kb on + strand, within RALBFv3_RS01450 at 326.849 kb on - strand, within RALBFv3_RS01450 at 326.853 kb on + strand, within RALBFv3_RS01450 at 327.467 kb on + strand, within RALBFv3_RS01450 at 327.467 kb on + strand, within RALBFv3_RS01450 at 327.468 kb on - strand, within RALBFv3_RS01450 at 327.556 kb on - strand, within RALBFv3_RS01450
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 323,130 - +0.8 323,249 + -0.3 323,249 + -0.3 323,628 + RALBFv3_RS01445 0.12 -2.5 323,986 - RALBFv3_RS01445 0.22 +1.0 323,986 - RALBFv3_RS01445 0.22 -0.8 324,028 - RALBFv3_RS01445 0.23 -0.7 324,211 - RALBFv3_RS01445 0.29 -0.9 324,528 + RALBFv3_RS01445 0.38 -1.5 324,777 + RALBFv3_RS01445 0.45 -0.5 324,798 + RALBFv3_RS01445 0.46 -1.7 324,798 + RALBFv3_RS01445 0.46 +0.5 324,799 - RALBFv3_RS01445 0.46 -0.9 324,803 - RALBFv3_RS01445 0.46 +0.0 324,803 - RALBFv3_RS01445 0.46 +1.0 324,803 - RALBFv3_RS01445 0.46 -0.4 324,955 - RALBFv3_RS01445 0.50 -1.0 325,972 - RALBFv3_RS01445 0.80 +0.0 325,978 - RALBFv3_RS01445 0.80 -0.9 326,071 - RALBFv3_RS01445 0.83 -0.0 326,163 + RALBFv3_RS01445 0.85 +1.0 326,848 + RALBFv3_RS01450 0.12 -0.6 326,848 + RALBFv3_RS01450 0.12 -0.1 326,848 + RALBFv3_RS01450 0.12 +0.2 326,848 + RALBFv3_RS01450 0.12 -0.9 326,849 - RALBFv3_RS01450 0.12 -0.4 326,853 + RALBFv3_RS01450 0.12 +0.3 327,467 + RALBFv3_RS01450 0.64 -0.8 327,467 + RALBFv3_RS01450 0.64 -0.0 327,468 - RALBFv3_RS01450 0.64 +0.4 327,556 - RALBFv3_RS01450 0.71 -0.3
Or see this region's nucleotide sequence