Strain Fitness in Ralstonia solanacearum IBSBF1503 around RALBFv3_RS01390

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRALBFv3_RS01385 and RALBFv3_RS01390 are separated by 67 nucleotidesRALBFv3_RS01390 and RALBFv3_RS01395 are separated by 134 nucleotides RALBFv3_RS01385: RALBFv3_RS01385 - AcrB/AcrD/AcrF family protein, at 309,224 to 312,343 _RS01385 RALBFv3_RS01390: RALBFv3_RS01390 - efflux RND transporter periplasmic adaptor subunit, at 312,411 to 313,619 _RS01390 RALBFv3_RS01395: RALBFv3_RS01395 - squalene synthase HpnD, at 313,754 to 314,626 _RS01395 Position (kb) 312 313 314Strain fitness (log2 ratio) -2 -1 0 1 2at 311.874 kb on + strand, within RALBFv3_RS01385at 311.901 kb on + strand, within RALBFv3_RS01385at 311.901 kb on + strand, within RALBFv3_RS01385at 312.243 kb on + strandat 312.784 kb on + strand, within RALBFv3_RS01390at 312.785 kb on - strand, within RALBFv3_RS01390at 312.785 kb on - strand, within RALBFv3_RS01390at 313.093 kb on + strand, within RALBFv3_RS01390at 313.115 kb on - strand, within RALBFv3_RS01390at 313.151 kb on + strand, within RALBFv3_RS01390at 313.542 kb on - strandat 313.652 kb on + strandat 313.653 kb on - strandat 313.751 kb on - strandat 313.751 kb on - strandat 313.808 kb on + strandat 313.848 kb on - strand, within RALBFv3_RS01395at 313.850 kb on + strand, within RALBFv3_RS01395at 314.315 kb on + strand, within RALBFv3_RS01395at 314.315 kb on + strand, within RALBFv3_RS01395at 314.316 kb on - strand, within RALBFv3_RS01395at 314.316 kb on - strand, within RALBFv3_RS01395

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
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311,874 + RALBFv3_RS01385 0.85 +1.2
311,901 + RALBFv3_RS01385 0.86 -0.8
311,901 + RALBFv3_RS01385 0.86 -0.5
312,243 + -1.9
312,784 + RALBFv3_RS01390 0.31 -0.9
312,785 - RALBFv3_RS01390 0.31 +0.1
312,785 - RALBFv3_RS01390 0.31 +2.6
313,093 + RALBFv3_RS01390 0.56 -1.3
313,115 - RALBFv3_RS01390 0.58 -1.0
313,151 + RALBFv3_RS01390 0.61 +1.1
313,542 - -0.1
313,652 + -1.9
313,653 - -0.5
313,751 - -0.3
313,751 - -0.6
313,808 + -0.3
313,848 - RALBFv3_RS01395 0.11 +1.5
313,850 + RALBFv3_RS01395 0.11 -0.5
314,315 + RALBFv3_RS01395 0.64 +0.5
314,315 + RALBFv3_RS01395 0.64 +0.3
314,316 - RALBFv3_RS01395 0.64 +0.5
314,316 - RALBFv3_RS01395 0.64 +1.1

Or see this region's nucleotide sequence