Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS01370 and RALBFv3_RS01375 are separated by 54 nucleotides RALBFv3_RS01375 and RALBFv3_RS01380 are separated by 180 nucleotides RALBFv3_RS01380 and RALBFv3_RS01385 are separated by 104 nucleotides RALBFv3_RS01385 and RALBFv3_RS01390 are separated by 67 nucleotides
RALBFv3_RS01370: RALBFv3_RS01370 - hypothetical protein, at 308,219 to 308,455
_RS01370
RALBFv3_RS01375: RALBFv3_RS01375 - hypothetical protein, at 308,510 to 308,852
_RS01375
RALBFv3_RS01380: RALBFv3_RS01380 - tRNA-Leu, at 309,033 to 309,119
_RS01380
RALBFv3_RS01385: RALBFv3_RS01385 - AcrB/AcrD/AcrF family protein, at 309,224 to 312,343
_RS01385
RALBFv3_RS01390: RALBFv3_RS01390 - efflux RND transporter periplasmic adaptor subunit, at 312,411 to 313,619
_RS01390
Position (kb) 309
310
311
312
313 Strain fitness (log2 ratio) -2
-1
0
1
2 at 308.317 kb on - strand, within RALBFv3_RS01370 at 308.322 kb on + strand, within RALBFv3_RS01370 at 308.322 kb on + strand, within RALBFv3_RS01370 at 308.323 kb on - strand, within RALBFv3_RS01370 at 308.323 kb on - strand, within RALBFv3_RS01370 at 308.634 kb on + strand, within RALBFv3_RS01375 at 308.649 kb on + strand, within RALBFv3_RS01375 at 308.670 kb on + strand, within RALBFv3_RS01375 at 308.671 kb on - strand, within RALBFv3_RS01375 at 308.730 kb on + strand, within RALBFv3_RS01375 at 309.172 kb on + strand at 309.173 kb on - strand at 310.665 kb on + strand, within RALBFv3_RS01385 at 310.665 kb on + strand, within RALBFv3_RS01385 at 310.836 kb on + strand, within RALBFv3_RS01385 at 310.920 kb on + strand, within RALBFv3_RS01385 at 310.981 kb on + strand, within RALBFv3_RS01385 at 311.874 kb on + strand, within RALBFv3_RS01385 at 311.901 kb on + strand, within RALBFv3_RS01385 at 311.901 kb on + strand, within RALBFv3_RS01385 at 312.243 kb on + strand at 312.784 kb on + strand, within RALBFv3_RS01390 at 312.785 kb on - strand, within RALBFv3_RS01390 at 312.785 kb on - strand, within RALBFv3_RS01390 at 313.093 kb on + strand, within RALBFv3_RS01390 at 313.115 kb on - strand, within RALBFv3_RS01390 at 313.151 kb on + strand, within RALBFv3_RS01390
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 308,317 - RALBFv3_RS01370 0.41 +0.9 308,322 + RALBFv3_RS01370 0.43 -0.3 308,322 + RALBFv3_RS01370 0.43 -0.5 308,323 - RALBFv3_RS01370 0.44 +0.3 308,323 - RALBFv3_RS01370 0.44 -1.2 308,634 + RALBFv3_RS01375 0.36 -0.6 308,649 + RALBFv3_RS01375 0.41 +0.2 308,670 + RALBFv3_RS01375 0.47 -0.6 308,671 - RALBFv3_RS01375 0.47 -0.4 308,730 + RALBFv3_RS01375 0.64 -0.6 309,172 + +0.1 309,173 - -0.4 310,665 + RALBFv3_RS01385 0.46 -0.3 310,665 + RALBFv3_RS01385 0.46 -1.9 310,836 + RALBFv3_RS01385 0.52 +0.1 310,920 + RALBFv3_RS01385 0.54 -0.3 310,981 + RALBFv3_RS01385 0.56 -0.9 311,874 + RALBFv3_RS01385 0.85 +1.2 311,901 + RALBFv3_RS01385 0.86 -0.8 311,901 + RALBFv3_RS01385 0.86 -0.5 312,243 + -1.9 312,784 + RALBFv3_RS01390 0.31 -0.9 312,785 - RALBFv3_RS01390 0.31 +0.1 312,785 - RALBFv3_RS01390 0.31 +2.6 313,093 + RALBFv3_RS01390 0.56 -1.3 313,115 - RALBFv3_RS01390 0.58 -1.0 313,151 + RALBFv3_RS01390 0.61 +1.1
Or see this region's nucleotide sequence