Strain Fitness in Ralstonia solanacearum IBSBF1503 around RALBFv3_RS01300

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRALBFv3_RS01295 and RALBFv3_RS01300 are separated by 61 nucleotidesRALBFv3_RS01300 and RALBFv3_RS01305 are separated by 12 nucleotidesRALBFv3_RS01305 and RALBFv3_RS01310 are separated by 76 nucleotides RALBFv3_RS01295: RALBFv3_RS01295 - bifunctional ADP-dependent NAD(P)H-hydrate dehydratase/NAD(P)H-hydrate epimerase, at 291,175 to 292,770 _RS01295 RALBFv3_RS01300: RALBFv3_RS01300 - glucose-6-phosphate isomerase, at 292,832 to 294,451 _RS01300 RALBFv3_RS01305: RALBFv3_RS01305 - ABC transporter ATP-binding protein, at 294,464 to 295,174 _RS01305 RALBFv3_RS01310: RALBFv3_RS01310 - arylesterase, at 295,251 to 295,868 _RS01310 Position (kb) 292 293 294 295Strain fitness (log2 ratio) -2 -1 0 1at 292.208 kb on - strand, within RALBFv3_RS01295at 292.795 kb on - strandat 293.093 kb on - strand, within RALBFv3_RS01300at 293.837 kb on + strand, within RALBFv3_RS01300at 293.987 kb on + strand, within RALBFv3_RS01300at 295.096 kb on + strand, within RALBFv3_RS01305at 295.096 kb on + strand, within RALBFv3_RS01305at 295.191 kb on - strandat 295.193 kb on + strandat 295.194 kb on - strandat 295.416 kb on + strand, within RALBFv3_RS01310

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
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292,208 - RALBFv3_RS01295 0.65 -0.3
292,795 - -2.1
293,093 - RALBFv3_RS01300 0.16 -2.5
293,837 + RALBFv3_RS01300 0.62 -0.5
293,987 + RALBFv3_RS01300 0.71 +0.1
295,096 + RALBFv3_RS01305 0.89 -0.1
295,096 + RALBFv3_RS01305 0.89 -0.6
295,191 - -0.2
295,193 + +1.1
295,194 - +0.7
295,416 + RALBFv3_RS01310 0.27 -0.3

Or see this region's nucleotide sequence