Strain Fitness in Ralstonia solanacearum IBSBF1503 around RALBFv3_RS00935

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRALBFv3_RS00930 and RALBFv3_RS00935 are separated by 141 nucleotidesRALBFv3_RS00935 and RALBFv3_RS00940 are separated by 211 nucleotides RALBFv3_RS00930: RALBFv3_RS00930 - GALA protein, at 205,456 to 207,063 _RS00930 RALBFv3_RS00935: RALBFv3_RS00935 - GALA protein, at 207,205 to 208,593 _RS00935 RALBFv3_RS00940: RALBFv3_RS00940 - 2-oxo acid dehydrogenase subunit E2, at 208,805 to 209,929 _RS00940 Position (kb) 207 208 209Strain fitness (log2 ratio) -1 0 1at 206.772 kb on + strand, within RALBFv3_RS00930at 207.702 kb on - strand, within RALBFv3_RS00935at 208.379 kb on - strand, within RALBFv3_RS00935at 208.623 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
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206,772 + RALBFv3_RS00930 0.82 -0.5
207,702 - RALBFv3_RS00935 0.36 +1.0
208,379 - RALBFv3_RS00935 0.85 -0.9
208,623 + -1.3

Or see this region's nucleotide sequence