Strain Fitness in Ralstonia solanacearum IBSBF1503 around RALBFv3_RS00290

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRALBFv3_RS00285 and RALBFv3_RS00290 are separated by 706 nucleotidesRALBFv3_RS00290 and RALBFv3_RS00295 are separated by 8 nucleotidesRALBFv3_RS00295 and RALBFv3_RS00300 are separated by 11 nucleotides RALBFv3_RS00285: RALBFv3_RS00285 - TolC family protein, at 64,649 to 66,037 _RS00285 RALBFv3_RS00290: RALBFv3_RS00290 - 50S ribosomal protein L3 N(5)-glutamine methyltransferase, at 66,744 to 67,646 _RS00290 RALBFv3_RS00295: RALBFv3_RS00295 - CYTH domain-containing protein, at 67,655 to 68,173 _RS00295 RALBFv3_RS00300: RALBFv3_RS00300 - succinyl-diaminopimelate desuccinylase, at 68,185 to 69,336 _RS00300 Position (kb) 66 67 68Strain fitness (log2 ratio) -1 0 1at 66.217 kb on + strandat 66.394 kb on + strandat 66.394 kb on + strandat 66.549 kb on + strandat 66.549 kb on + strandat 66.550 kb on - strandat 66.605 kb on + strandat 67.039 kb on + strand, within RALBFv3_RS00290at 67.040 kb on - strand, within RALBFv3_RS00290at 67.066 kb on + strand, within RALBFv3_RS00290at 67.066 kb on + strand, within RALBFv3_RS00290

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
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66,217 + +0.4
66,394 + +0.0
66,394 + -0.4
66,549 + -0.5
66,549 + +0.6
66,550 - -0.9
66,605 + -0.8
67,039 + RALBFv3_RS00290 0.33 -1.1
67,040 - RALBFv3_RS00290 0.33 -0.9
67,066 + RALBFv3_RS00290 0.36 -1.1
67,066 + RALBFv3_RS00290 0.36 -0.1

Or see this region's nucleotide sequence