Strain Fitness in Ralstonia solanacearum IBSBF1503 around RALBFv3_RS00235

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRALBFv3_RS00230 and RALBFv3_RS00235 are separated by 26 nucleotidesRALBFv3_RS00235 and RALBFv3_RS00240 are separated by 37 nucleotides RALBFv3_RS00230: RALBFv3_RS00230 - MurR/RpiR family transcriptional regulator, at 50,462 to 51,469 _RS00230 RALBFv3_RS00235: RALBFv3_RS00235 - isoaspartyl peptidase/L-asparaginase, at 51,496 to 52,458 _RS00235 RALBFv3_RS00240: RALBFv3_RS00240 - glutathione ABC transporter ATP-binding protein, at 52,496 to 54,376 _RS00240 Position (kb) 51 52 53Strain fitness (log2 ratio) -2 -1 0 1 2at 50.593 kb on - strand, within RALBFv3_RS00230at 51.495 kb on + strandat 51.496 kb on - strandat 51.733 kb on + strand, within RALBFv3_RS00235at 51.734 kb on - strand, within RALBFv3_RS00235at 51.925 kb on + strand, within RALBFv3_RS00235at 51.928 kb on + strand, within RALBFv3_RS00235at 51.928 kb on + strand, within RALBFv3_RS00235at 51.929 kb on - strand, within RALBFv3_RS00235at 52.178 kb on - strand, within RALBFv3_RS00235at 52.246 kb on + strand, within RALBFv3_RS00235at 53.237 kb on + strand, within RALBFv3_RS00240at 53.237 kb on + strand, within RALBFv3_RS00240at 53.237 kb on + strand, within RALBFv3_RS00240at 53.238 kb on - strand, within RALBFv3_RS00240at 53.238 kb on - strand, within RALBFv3_RS00240at 53.300 kb on + strand, within RALBFv3_RS00240at 53.301 kb on - strand, within RALBFv3_RS00240

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
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50,593 - RALBFv3_RS00230 0.13 +1.5
51,495 + +0.6
51,496 - +0.1
51,733 + RALBFv3_RS00235 0.25 -1.3
51,734 - RALBFv3_RS00235 0.25 -0.5
51,925 + RALBFv3_RS00235 0.45 -2.0
51,928 + RALBFv3_RS00235 0.45 -1.2
51,928 + RALBFv3_RS00235 0.45 -2.2
51,929 - RALBFv3_RS00235 0.45 -0.2
52,178 - RALBFv3_RS00235 0.71 -0.3
52,246 + RALBFv3_RS00235 0.78 -1.0
53,237 + RALBFv3_RS00240 0.39 +1.0
53,237 + RALBFv3_RS00240 0.39 +0.5
53,237 + RALBFv3_RS00240 0.39 -0.3
53,238 - RALBFv3_RS00240 0.39 +0.1
53,238 - RALBFv3_RS00240 0.39 +1.9
53,300 + RALBFv3_RS00240 0.43 -0.2
53,301 - RALBFv3_RS00240 0.43 +1.0

Or see this region's nucleotide sequence