Strain Fitness in Ralstonia solanacearum IBSBF1503 around RALBFv3_RS00200

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRALBFv3_RS00190 and RALBFv3_RS00195 are separated by 70 nucleotidesRALBFv3_RS00195 and RALBFv3_RS00200 are separated by 65 nucleotidesRALBFv3_RS00200 and RALBFv3_RS00205 are separated by 625 nucleotides RALBFv3_RS00190: RALBFv3_RS00190 - ribosomal-protein-alanine N-acetyltransferase, at 40,112 to 40,639 _RS00190 RALBFv3_RS00195: RALBFv3_RS00195 - uracil-DNA glycosylase, at 40,710 to 41,555 _RS00195 RALBFv3_RS00200: RALBFv3_RS00200 - lysophospholipid transporter LplT, at 41,621 to 42,937 _RS00200 RALBFv3_RS00205: RALBFv3_RS00205 - alanine racemase, at 43,563 to 44,690 _RS00205 Position (kb) 41 42 43Strain fitness (log2 ratio) -1 0 1at 41.554 kb on - strandat 41.779 kb on - strand, within RALBFv3_RS00200at 41.952 kb on + strand, within RALBFv3_RS00200at 42.012 kb on + strand, within RALBFv3_RS00200at 42.394 kb on - strand, within RALBFv3_RS00200at 42.624 kb on + strand, within RALBFv3_RS00200at 42.922 kb on - strandat 42.957 kb on + strandat 43.223 kb on + strandat 43.298 kb on + strandat 43.444 kb on + strandat 43.445 kb on - strandat 43.500 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
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41,554 - -1.6
41,779 - RALBFv3_RS00200 0.12 -0.3
41,952 + RALBFv3_RS00200 0.25 -0.7
42,012 + RALBFv3_RS00200 0.30 +1.0
42,394 - RALBFv3_RS00200 0.59 -1.5
42,624 + RALBFv3_RS00200 0.76 -1.5
42,922 - -1.3
42,957 + -0.4
43,223 + -0.1
43,298 + -1.2
43,444 + -0.4
43,445 - -0.4
43,500 + +0.3

Or see this region's nucleotide sequence