Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Penicillin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS09325 and RALBFv3_RS23850 are separated by 639 nucleotides RALBFv3_RS23850 and RALBFv3_RS09330 are separated by 166 nucleotides
RALBFv3_RS09325: RALBFv3_RS09325 - hypothetical protein, at 2,066,600 to 2,066,851
_RS09325
RALBFv3_RS23850: RALBFv3_RS23850 - hypothetical protein, at 2,067,491 to 2,067,955
_RS23850
RALBFv3_RS09330: RALBFv3_RS09330 - recombinase family protein, at 2,068,122 to 2,069,141
_RS09330
Position (kb)
2067
2068 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2066.519 kb on + strand at 2066.520 kb on - strand at 2066.520 kb on - strand at 2066.681 kb on + strand, within RALBFv3_RS09325 at 2066.716 kb on + strand, within RALBFv3_RS09325 at 2066.716 kb on + strand, within RALBFv3_RS09325 at 2066.716 kb on + strand, within RALBFv3_RS09325 at 2066.716 kb on + strand, within RALBFv3_RS09325 at 2066.717 kb on - strand, within RALBFv3_RS09325 at 2066.717 kb on - strand, within RALBFv3_RS09325 at 2066.722 kb on + strand, within RALBFv3_RS09325 at 2066.722 kb on + strand, within RALBFv3_RS09325 at 2066.722 kb on + strand, within RALBFv3_RS09325 at 2066.722 kb on + strand, within RALBFv3_RS09325 at 2066.723 kb on - strand, within RALBFv3_RS09325 at 2066.758 kb on + strand, within RALBFv3_RS09325 at 2066.758 kb on + strand, within RALBFv3_RS09325 at 2066.758 kb on + strand, within RALBFv3_RS09325 at 2066.758 kb on + strand, within RALBFv3_RS09325 at 2066.758 kb on + strand, within RALBFv3_RS09325 at 2066.758 kb on + strand, within RALBFv3_RS09325 at 2066.759 kb on - strand, within RALBFv3_RS09325 at 2066.759 kb on - strand, within RALBFv3_RS09325 at 2066.768 kb on + strand, within RALBFv3_RS09325 at 2066.769 kb on - strand, within RALBFv3_RS09325 at 2066.769 kb on - strand, within RALBFv3_RS09325 at 2066.969 kb on + strand at 2066.970 kb on - strand at 2066.984 kb on + strand at 2066.985 kb on - strand at 2067.001 kb on + strand at 2067.001 kb on + strand at 2067.001 kb on + strand at 2067.002 kb on - strand at 2067.003 kb on + strand at 2067.003 kb on + strand at 2067.003 kb on + strand at 2067.003 kb on + strand at 2067.004 kb on - strand at 2067.005 kb on + strand at 2067.005 kb on + strand at 2067.005 kb on + strand at 2067.006 kb on - strand at 2067.006 kb on - strand at 2067.006 kb on - strand at 2067.006 kb on - strand at 2067.006 kb on - strand at 2067.041 kb on - strand at 2067.125 kb on + strand at 2067.141 kb on - strand at 2067.154 kb on + strand at 2067.177 kb on - strand at 2067.184 kb on + strand at 2067.285 kb on + strand at 2067.285 kb on + strand at 2067.351 kb on + strand at 2067.351 kb on + strand at 2067.351 kb on + strand at 2067.351 kb on + strand at 2067.352 kb on - strand at 2067.352 kb on - strand at 2067.352 kb on - strand at 2067.352 kb on - strand at 2067.496 kb on + strand at 2067.496 kb on + strand at 2067.496 kb on + strand at 2067.496 kb on + strand at 2067.496 kb on + strand at 2067.496 kb on + strand at 2067.497 kb on - strand at 2067.497 kb on - strand at 2067.497 kb on - strand at 2067.497 kb on - strand at 2067.498 kb on - strand at 2067.625 kb on + strand, within RALBFv3_RS23850 at 2067.626 kb on - strand, within RALBFv3_RS23850 at 2067.638 kb on + strand, within RALBFv3_RS23850 at 2067.692 kb on + strand, within RALBFv3_RS23850 at 2067.693 kb on - strand, within RALBFv3_RS23850 at 2067.693 kb on - strand, within RALBFv3_RS23850 at 2067.693 kb on - strand, within RALBFv3_RS23850 at 2067.705 kb on - strand, within RALBFv3_RS23850 at 2067.725 kb on + strand, within RALBFv3_RS23850 at 2067.726 kb on - strand, within RALBFv3_RS23850 at 2067.726 kb on - strand, within RALBFv3_RS23850 at 2067.836 kb on + strand, within RALBFv3_RS23850 at 2067.836 kb on + strand, within RALBFv3_RS23850 at 2067.836 kb on + strand, within RALBFv3_RS23850 at 2067.836 kb on + strand, within RALBFv3_RS23850 at 2067.836 kb on + strand, within RALBFv3_RS23850 at 2067.836 kb on + strand, within RALBFv3_RS23850 at 2067.836 kb on + strand, within RALBFv3_RS23850 at 2067.836 kb on + strand, within RALBFv3_RS23850 at 2067.836 kb on + strand, within RALBFv3_RS23850 at 2067.837 kb on - strand, within RALBFv3_RS23850 at 2067.837 kb on - strand, within RALBFv3_RS23850 at 2067.837 kb on - strand, within RALBFv3_RS23850 at 2067.837 kb on - strand, within RALBFv3_RS23850 at 2067.917 kb on - strand at 2067.978 kb on + strand at 2067.979 kb on - strand at 2067.992 kb on + strand at 2067.992 kb on + strand at 2068.032 kb on + strand at 2068.033 kb on - strand at 2068.037 kb on + strand at 2068.185 kb on + strand at 2068.185 kb on + strand at 2068.185 kb on + strand at 2068.186 kb on - strand at 2068.186 kb on - strand at 2068.186 kb on - strand at 2068.186 kb on - strand at 2068.186 kb on - strand at 2068.215 kb on + strand at 2068.216 kb on - strand at 2068.216 kb on - strand at 2068.287 kb on + strand, within RALBFv3_RS09330 at 2068.395 kb on + strand, within RALBFv3_RS09330 at 2068.395 kb on + strand, within RALBFv3_RS09330 at 2068.443 kb on + strand, within RALBFv3_RS09330 at 2068.443 kb on + strand, within RALBFv3_RS09330 at 2068.443 kb on + strand, within RALBFv3_RS09330 at 2068.444 kb on - strand, within RALBFv3_RS09330 at 2068.446 kb on + strand, within RALBFv3_RS09330 at 2068.446 kb on + strand, within RALBFv3_RS09330 at 2068.447 kb on - strand, within RALBFv3_RS09330 at 2068.447 kb on - strand, within RALBFv3_RS09330 at 2068.452 kb on + strand, within RALBFv3_RS09330 at 2068.452 kb on + strand, within RALBFv3_RS09330 at 2068.452 kb on + strand, within RALBFv3_RS09330 at 2068.452 kb on + strand, within RALBFv3_RS09330 at 2068.452 kb on + strand, within RALBFv3_RS09330 at 2068.453 kb on - strand, within RALBFv3_RS09330 at 2068.453 kb on - strand, within RALBFv3_RS09330 at 2068.453 kb on - strand, within RALBFv3_RS09330 at 2068.453 kb on - strand, within RALBFv3_RS09330 at 2068.453 kb on - strand, within RALBFv3_RS09330 at 2068.454 kb on + strand, within RALBFv3_RS09330 at 2068.454 kb on + strand, within RALBFv3_RS09330 at 2068.454 kb on + strand, within RALBFv3_RS09330 at 2068.454 kb on + strand, within RALBFv3_RS09330 at 2068.454 kb on + strand, within RALBFv3_RS09330 at 2068.455 kb on - strand, within RALBFv3_RS09330 at 2068.455 kb on - strand, within RALBFv3_RS09330 at 2068.455 kb on - strand, within RALBFv3_RS09330 at 2068.485 kb on + strand, within RALBFv3_RS09330 at 2068.485 kb on + strand, within RALBFv3_RS09330 at 2068.486 kb on - strand, within RALBFv3_RS09330 at 2068.566 kb on + strand, within RALBFv3_RS09330 at 2068.567 kb on - strand, within RALBFv3_RS09330 at 2068.567 kb on - strand, within RALBFv3_RS09330 at 2068.567 kb on - strand, within RALBFv3_RS09330 at 2068.567 kb on - strand, within RALBFv3_RS09330 at 2068.567 kb on - strand, within RALBFv3_RS09330 at 2068.568 kb on - strand, within RALBFv3_RS09330 at 2068.651 kb on - strand, within RALBFv3_RS09330 at 2068.666 kb on + strand, within RALBFv3_RS09330 at 2068.666 kb on + strand, within RALBFv3_RS09330 at 2068.666 kb on + strand, within RALBFv3_RS09330 at 2068.666 kb on + strand, within RALBFv3_RS09330 at 2068.791 kb on + strand, within RALBFv3_RS09330 at 2068.791 kb on + strand, within RALBFv3_RS09330 at 2068.791 kb on + strand, within RALBFv3_RS09330 at 2068.791 kb on + strand, within RALBFv3_RS09330 at 2068.792 kb on - strand, within RALBFv3_RS09330 at 2068.906 kb on + strand, within RALBFv3_RS09330 at 2068.906 kb on + strand, within RALBFv3_RS09330 at 2068.906 kb on + strand, within RALBFv3_RS09330 at 2068.907 kb on - strand, within RALBFv3_RS09330 at 2068.907 kb on - strand, within RALBFv3_RS09330 at 2068.923 kb on + strand, within RALBFv3_RS09330 at 2068.923 kb on + strand, within RALBFv3_RS09330 at 2068.924 kb on - strand, within RALBFv3_RS09330 at 2068.941 kb on + strand, within RALBFv3_RS09330 at 2068.941 kb on + strand, within RALBFv3_RS09330 at 2068.942 kb on - strand, within RALBFv3_RS09330 at 2068.942 kb on - strand, within RALBFv3_RS09330 at 2068.942 kb on - strand, within RALBFv3_RS09330 at 2068.942 kb on - strand, within RALBFv3_RS09330 at 2068.942 kb on - strand, within RALBFv3_RS09330 at 2068.942 kb on - strand, within RALBFv3_RS09330 at 2068.942 kb on - strand, within RALBFv3_RS09330 at 2068.942 kb on - strand, within RALBFv3_RS09330 at 2068.942 kb on - strand, within RALBFv3_RS09330 at 2068.943 kb on + strand, within RALBFv3_RS09330
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Penicillin remove 2,066,519 + +0.2 2,066,520 - +0.8 2,066,520 - +0.7 2,066,681 + RALBFv3_RS09325 0.32 -0.1 2,066,716 + RALBFv3_RS09325 0.46 -0.5 2,066,716 + RALBFv3_RS09325 0.46 +1.3 2,066,716 + RALBFv3_RS09325 0.46 -0.2 2,066,716 + RALBFv3_RS09325 0.46 +0.3 2,066,717 - RALBFv3_RS09325 0.46 -0.3 2,066,717 - RALBFv3_RS09325 0.46 +0.6 2,066,722 + RALBFv3_RS09325 0.48 +1.5 2,066,722 + RALBFv3_RS09325 0.48 -0.3 2,066,722 + RALBFv3_RS09325 0.48 +3.3 2,066,722 + RALBFv3_RS09325 0.48 -0.2 2,066,723 - RALBFv3_RS09325 0.49 +1.3 2,066,758 + RALBFv3_RS09325 0.63 -1.4 2,066,758 + RALBFv3_RS09325 0.63 +0.2 2,066,758 + RALBFv3_RS09325 0.63 -0.7 2,066,758 + RALBFv3_RS09325 0.63 -1.5 2,066,758 + RALBFv3_RS09325 0.63 -0.3 2,066,758 + RALBFv3_RS09325 0.63 -0.5 2,066,759 - RALBFv3_RS09325 0.63 -0.0 2,066,759 - RALBFv3_RS09325 0.63 +0.2 2,066,768 + RALBFv3_RS09325 0.67 -0.2 2,066,769 - RALBFv3_RS09325 0.67 -0.1 2,066,769 - RALBFv3_RS09325 0.67 -1.5 2,066,969 + +1.3 2,066,970 - -0.3 2,066,984 + +0.2 2,066,985 - +1.3 2,067,001 + +2.3 2,067,001 + -0.0 2,067,001 + +0.2 2,067,002 - +2.1 2,067,003 + -1.3 2,067,003 + -0.6 2,067,003 + -0.3 2,067,003 + -0.4 2,067,004 - -0.9 2,067,005 + +0.0 2,067,005 + -0.0 2,067,005 + -0.9 2,067,006 - +0.7 2,067,006 - -1.2 2,067,006 - +0.5 2,067,006 - -0.6 2,067,006 - +1.3 2,067,041 - -1.4 2,067,125 + -0.2 2,067,141 - -0.5 2,067,154 + -1.0 2,067,177 - -0.1 2,067,184 + -1.2 2,067,285 + +0.1 2,067,285 + -0.3 2,067,351 + -0.3 2,067,351 + -0.4 2,067,351 + -0.3 2,067,351 + -0.3 2,067,352 - -0.6 2,067,352 - -1.9 2,067,352 - +0.0 2,067,352 - +0.2 2,067,496 + +0.5 2,067,496 + +0.3 2,067,496 + +0.1 2,067,496 + +0.5 2,067,496 + +0.3 2,067,496 + -0.4 2,067,497 - -0.5 2,067,497 - -0.2 2,067,497 - -2.0 2,067,497 - +0.3 2,067,498 - +0.9 2,067,625 + RALBFv3_RS23850 0.29 -1.2 2,067,626 - RALBFv3_RS23850 0.29 -0.3 2,067,638 + RALBFv3_RS23850 0.32 -0.1 2,067,692 + RALBFv3_RS23850 0.43 -0.7 2,067,693 - RALBFv3_RS23850 0.43 -1.5 2,067,693 - RALBFv3_RS23850 0.43 +0.1 2,067,693 - RALBFv3_RS23850 0.43 -0.2 2,067,705 - RALBFv3_RS23850 0.46 -0.3 2,067,725 + RALBFv3_RS23850 0.50 +1.3 2,067,726 - RALBFv3_RS23850 0.51 -0.2 2,067,726 - RALBFv3_RS23850 0.51 -0.2 2,067,836 + RALBFv3_RS23850 0.74 -0.1 2,067,836 + RALBFv3_RS23850 0.74 -0.7 2,067,836 + RALBFv3_RS23850 0.74 -0.6 2,067,836 + RALBFv3_RS23850 0.74 -0.2 2,067,836 + RALBFv3_RS23850 0.74 -0.1 2,067,836 + RALBFv3_RS23850 0.74 -1.0 2,067,836 + RALBFv3_RS23850 0.74 -0.6 2,067,836 + RALBFv3_RS23850 0.74 +0.3 2,067,836 + RALBFv3_RS23850 0.74 -0.0 2,067,837 - RALBFv3_RS23850 0.74 -0.1 2,067,837 - RALBFv3_RS23850 0.74 -0.9 2,067,837 - RALBFv3_RS23850 0.74 -0.3 2,067,837 - RALBFv3_RS23850 0.74 -0.2 2,067,917 - -0.1 2,067,978 + +0.3 2,067,979 - +0.7 2,067,992 + -0.3 2,067,992 + -1.0 2,068,032 + +0.1 2,068,033 - +0.5 2,068,037 + -0.5 2,068,185 + -0.2 2,068,185 + +0.0 2,068,185 + -0.7 2,068,186 - -0.0 2,068,186 - +1.3 2,068,186 - -0.3 2,068,186 - +0.7 2,068,186 - +0.1 2,068,215 + -0.2 2,068,216 - -0.6 2,068,216 - -0.2 2,068,287 + RALBFv3_RS09330 0.16 -1.9 2,068,395 + RALBFv3_RS09330 0.27 +0.2 2,068,395 + RALBFv3_RS09330 0.27 -0.5 2,068,443 + RALBFv3_RS09330 0.31 -0.7 2,068,443 + RALBFv3_RS09330 0.31 +0.1 2,068,443 + RALBFv3_RS09330 0.31 +0.1 2,068,444 - RALBFv3_RS09330 0.32 +0.5 2,068,446 + RALBFv3_RS09330 0.32 +0.1 2,068,446 + RALBFv3_RS09330 0.32 -0.0 2,068,447 - RALBFv3_RS09330 0.32 -0.2 2,068,447 - RALBFv3_RS09330 0.32 -0.6 2,068,452 + RALBFv3_RS09330 0.32 +0.6 2,068,452 + RALBFv3_RS09330 0.32 -0.3 2,068,452 + RALBFv3_RS09330 0.32 +0.4 2,068,452 + RALBFv3_RS09330 0.32 -0.6 2,068,452 + RALBFv3_RS09330 0.32 +0.0 2,068,453 - RALBFv3_RS09330 0.32 +0.1 2,068,453 - RALBFv3_RS09330 0.32 +0.5 2,068,453 - RALBFv3_RS09330 0.32 -0.6 2,068,453 - RALBFv3_RS09330 0.32 +0.4 2,068,453 - RALBFv3_RS09330 0.32 +0.1 2,068,454 + RALBFv3_RS09330 0.33 -1.1 2,068,454 + RALBFv3_RS09330 0.33 +1.3 2,068,454 + RALBFv3_RS09330 0.33 -1.2 2,068,454 + RALBFv3_RS09330 0.33 -0.3 2,068,454 + RALBFv3_RS09330 0.33 +0.3 2,068,455 - RALBFv3_RS09330 0.33 +1.1 2,068,455 - RALBFv3_RS09330 0.33 +0.4 2,068,455 - RALBFv3_RS09330 0.33 -0.5 2,068,485 + RALBFv3_RS09330 0.36 -1.2 2,068,485 + RALBFv3_RS09330 0.36 +1.3 2,068,486 - RALBFv3_RS09330 0.36 +0.1 2,068,566 + RALBFv3_RS09330 0.44 +0.7 2,068,567 - RALBFv3_RS09330 0.44 -1.6 2,068,567 - RALBFv3_RS09330 0.44 -0.2 2,068,567 - RALBFv3_RS09330 0.44 +0.3 2,068,567 - RALBFv3_RS09330 0.44 +1.3 2,068,567 - RALBFv3_RS09330 0.44 -0.7 2,068,568 - RALBFv3_RS09330 0.44 -1.4 2,068,651 - RALBFv3_RS09330 0.52 -0.2 2,068,666 + RALBFv3_RS09330 0.53 -1.2 2,068,666 + RALBFv3_RS09330 0.53 +1.3 2,068,666 + RALBFv3_RS09330 0.53 +0.1 2,068,666 + RALBFv3_RS09330 0.53 +0.2 2,068,791 + RALBFv3_RS09330 0.66 -0.8 2,068,791 + RALBFv3_RS09330 0.66 +0.5 2,068,791 + RALBFv3_RS09330 0.66 +0.4 2,068,791 + RALBFv3_RS09330 0.66 -0.5 2,068,792 - RALBFv3_RS09330 0.66 +1.3 2,068,906 + RALBFv3_RS09330 0.77 -1.2 2,068,906 + RALBFv3_RS09330 0.77 -0.3 2,068,906 + RALBFv3_RS09330 0.77 -0.4 2,068,907 - RALBFv3_RS09330 0.77 +0.4 2,068,907 - RALBFv3_RS09330 0.77 -0.3 2,068,923 + RALBFv3_RS09330 0.79 -0.5 2,068,923 + RALBFv3_RS09330 0.79 -0.8 2,068,924 - RALBFv3_RS09330 0.79 -0.3 2,068,941 + RALBFv3_RS09330 0.80 +1.3 2,068,941 + RALBFv3_RS09330 0.80 +0.3 2,068,942 - RALBFv3_RS09330 0.80 -0.7 2,068,942 - RALBFv3_RS09330 0.80 -0.2 2,068,942 - RALBFv3_RS09330 0.80 -0.2 2,068,942 - RALBFv3_RS09330 0.80 -0.5 2,068,942 - RALBFv3_RS09330 0.80 -0.2 2,068,942 - RALBFv3_RS09330 0.80 +0.3 2,068,942 - RALBFv3_RS09330 0.80 +0.4 2,068,942 - RALBFv3_RS09330 0.80 -0.1 2,068,942 - RALBFv3_RS09330 0.80 -0.1 2,068,943 + RALBFv3_RS09330 0.80 +1.3
Or see this region's nucleotide sequence