Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Penicillin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS07495 and RALBFv3_RS07500 are separated by 320 nucleotides RALBFv3_RS07500 and RALBFv3_RS07505 are separated by 46 nucleotides RALBFv3_RS07505 and RALBFv3_RS07510 are separated by 258 nucleotides
RALBFv3_RS07495: RALBFv3_RS07495 - hypothetical protein, at 1,649,337 to 1,649,762
_RS07495
RALBFv3_RS07500: RALBFv3_RS07500 - hypothetical protein, at 1,650,083 to 1,650,301
_RS07500
RALBFv3_RS07505: RALBFv3_RS07505 - hypothetical protein, at 1,650,348 to 1,650,569
_RS07505
RALBFv3_RS07510: RALBFv3_RS07510 - hypothetical protein, at 1,650,828 to 1,652,039
_RS07510
Position (kb)
1650
1651 Strain fitness (log2 ratio)
-2
-1
0
1 at 1649.533 kb on + strand, within RALBFv3_RS07495 at 1649.533 kb on + strand, within RALBFv3_RS07495 at 1649.566 kb on + strand, within RALBFv3_RS07495 at 1649.566 kb on + strand, within RALBFv3_RS07495 at 1649.567 kb on - strand, within RALBFv3_RS07495 at 1649.567 kb on - strand, within RALBFv3_RS07495 at 1649.600 kb on - strand, within RALBFv3_RS07495 at 1649.657 kb on - strand, within RALBFv3_RS07495 at 1649.725 kb on + strand at 1649.725 kb on + strand at 1649.746 kb on + strand at 1649.845 kb on + strand at 1649.845 kb on + strand at 1649.852 kb on + strand at 1649.852 kb on + strand at 1649.853 kb on - strand at 1649.853 kb on - strand at 1649.904 kb on - strand at 1649.968 kb on + strand at 1650.072 kb on - strand at 1650.084 kb on + strand at 1650.084 kb on + strand at 1650.311 kb on + strand at 1650.311 kb on + strand at 1650.311 kb on + strand at 1650.311 kb on + strand at 1650.311 kb on + strand at 1650.312 kb on - strand at 1650.349 kb on + strand at 1650.349 kb on + strand at 1650.349 kb on + strand at 1650.407 kb on - strand, within RALBFv3_RS07505 at 1650.697 kb on + strand at 1650.698 kb on - strand at 1650.883 kb on + strand at 1650.883 kb on + strand at 1650.883 kb on + strand at 1650.884 kb on - strand at 1650.884 kb on - strand at 1651.062 kb on + strand, within RALBFv3_RS07510 at 1651.062 kb on + strand, within RALBFv3_RS07510 at 1651.237 kb on - strand, within RALBFv3_RS07510 at 1651.338 kb on + strand, within RALBFv3_RS07510 at 1651.338 kb on + strand, within RALBFv3_RS07510 at 1651.378 kb on + strand, within RALBFv3_RS07510 at 1651.410 kb on + strand, within RALBFv3_RS07510 at 1651.432 kb on + strand, within RALBFv3_RS07510 at 1651.433 kb on - strand, within RALBFv3_RS07510 at 1651.433 kb on - strand, within RALBFv3_RS07510
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Penicillin remove 1,649,533 + RALBFv3_RS07495 0.46 +1.1 1,649,533 + RALBFv3_RS07495 0.46 -1.1 1,649,566 + RALBFv3_RS07495 0.54 -0.3 1,649,566 + RALBFv3_RS07495 0.54 -0.3 1,649,567 - RALBFv3_RS07495 0.54 -1.0 1,649,567 - RALBFv3_RS07495 0.54 -0.0 1,649,600 - RALBFv3_RS07495 0.62 -0.7 1,649,657 - RALBFv3_RS07495 0.75 -0.2 1,649,725 + -0.4 1,649,725 + -0.1 1,649,746 + -0.4 1,649,845 + -0.1 1,649,845 + -1.0 1,649,852 + +1.3 1,649,852 + -1.2 1,649,853 - +0.1 1,649,853 - -0.3 1,649,904 - +0.1 1,649,968 + -0.3 1,650,072 - +0.9 1,650,084 + +0.3 1,650,084 + +0.3 1,650,311 + +0.4 1,650,311 + +0.7 1,650,311 + -1.4 1,650,311 + +0.8 1,650,311 + +1.3 1,650,312 - +0.3 1,650,349 + -0.4 1,650,349 + -0.9 1,650,349 + -0.4 1,650,407 - RALBFv3_RS07505 0.27 +0.0 1,650,697 + -0.2 1,650,698 - +0.1 1,650,883 + -2.4 1,650,883 + +1.5 1,650,883 + -0.1 1,650,884 - -0.1 1,650,884 - +0.2 1,651,062 + RALBFv3_RS07510 0.19 -0.2 1,651,062 + RALBFv3_RS07510 0.19 +1.3 1,651,237 - RALBFv3_RS07510 0.34 +0.9 1,651,338 + RALBFv3_RS07510 0.42 +0.3 1,651,338 + RALBFv3_RS07510 0.42 +0.9 1,651,378 + RALBFv3_RS07510 0.45 -0.5 1,651,410 + RALBFv3_RS07510 0.48 -2.3 1,651,432 + RALBFv3_RS07510 0.50 -0.8 1,651,433 - RALBFv3_RS07510 0.50 -0.7 1,651,433 - RALBFv3_RS07510 0.50 +1.0
Or see this region's nucleotide sequence