Strain Fitness in Ralstonia solanacearum IBSBF1503 around RALBFv3_RS12700

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRALBFv3_RS12695 and RALBFv3_RS12700 are separated by 40 nucleotidesRALBFv3_RS12700 and RALBFv3_RS12705 overlap by 1 nucleotides RALBFv3_RS12695: RALBFv3_RS12695 - phosphomannomutase/phosphoglucomutase, at 2,796,754 to 2,798,139 _RS12695 RALBFv3_RS12700: RALBFv3_RS12700 - lipopolysaccharide heptosyltransferase I, at 2,798,180 to 2,799,178 _RS12700 RALBFv3_RS12705: RALBFv3_RS12705 - 3-deoxy-D-manno-octulosonic acid transferase, at 2,799,178 to 2,800,494 _RS12705 Position (kb) 2798 2799 2800Strain fitness (log2 ratio) -3 -2 -1 0 1at 2797.195 kb on + strand, within RALBFv3_RS12695at 2797.734 kb on + strand, within RALBFv3_RS12695at 2798.579 kb on + strand, within RALBFv3_RS12700at 2798.579 kb on + strand, within RALBFv3_RS12700at 2799.050 kb on + strand, within RALBFv3_RS12700

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol
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2,797,195 + RALBFv3_RS12695 0.32 -0.5
2,797,734 + RALBFv3_RS12695 0.71 -1.0
2,798,579 + RALBFv3_RS12700 0.40 -1.3
2,798,579 + RALBFv3_RS12700 0.40 -3.5
2,799,050 + RALBFv3_RS12700 0.87 -3.5

Or see this region's nucleotide sequence