Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS09520 and RALBFv3_RS09525 are separated by 167 nucleotides RALBFv3_RS09525 and RALBFv3_RS09530 are separated by 231 nucleotides RALBFv3_RS09530 and RALBFv3_RS09535 overlap by 4 nucleotides
RALBFv3_RS09520: RALBFv3_RS09520 - sel1 repeat family protein, at 2,121,180 to 2,121,938
_RS09520
RALBFv3_RS09525: RALBFv3_RS09525 - polyhydroxybutyrate granule-associated protein (phasin) phap2, at 2,122,106 to 2,122,627
_RS09525
RALBFv3_RS09530: RALBFv3_RS09530 - methionine biosynthesis protein MetW, at 2,122,859 to 2,123,506
_RS09530
RALBFv3_RS09535: RALBFv3_RS09535 - homoserine O-acetyltransferase, at 2,123,503 to 2,124,714
_RS09535
Position (kb)
2122
2123 Strain fitness (log2 ratio)
-1
0
1 at 2121.108 kb on + strand at 2121.181 kb on + strand at 2121.181 kb on + strand at 2121.182 kb on - strand at 2121.182 kb on - strand at 2121.182 kb on - strand at 2121.182 kb on - strand at 2121.182 kb on - strand at 2121.182 kb on - strand at 2121.230 kb on - strand at 2121.230 kb on - strand at 2121.230 kb on - strand at 2121.356 kb on - strand, within RALBFv3_RS09520 at 2121.356 kb on - strand, within RALBFv3_RS09520 at 2121.358 kb on + strand, within RALBFv3_RS09520 at 2121.359 kb on - strand, within RALBFv3_RS09520 at 2121.359 kb on - strand, within RALBFv3_RS09520 at 2121.372 kb on + strand, within RALBFv3_RS09520 at 2121.373 kb on + strand, within RALBFv3_RS09520 at 2121.373 kb on + strand, within RALBFv3_RS09520 at 2121.373 kb on + strand, within RALBFv3_RS09520 at 2121.373 kb on + strand, within RALBFv3_RS09520 at 2121.373 kb on + strand, within RALBFv3_RS09520 at 2121.374 kb on - strand, within RALBFv3_RS09520 at 2121.374 kb on - strand, within RALBFv3_RS09520 at 2121.412 kb on + strand, within RALBFv3_RS09520 at 2121.463 kb on + strand, within RALBFv3_RS09520 at 2121.463 kb on + strand, within RALBFv3_RS09520 at 2121.463 kb on + strand, within RALBFv3_RS09520 at 2121.463 kb on + strand, within RALBFv3_RS09520 at 2121.464 kb on - strand, within RALBFv3_RS09520 at 2121.464 kb on - strand, within RALBFv3_RS09520 at 2121.520 kb on + strand, within RALBFv3_RS09520 at 2121.521 kb on - strand, within RALBFv3_RS09520 at 2121.583 kb on + strand, within RALBFv3_RS09520 at 2121.691 kb on + strand, within RALBFv3_RS09520 at 2121.692 kb on - strand, within RALBFv3_RS09520 at 2121.772 kb on + strand, within RALBFv3_RS09520 at 2121.972 kb on + strand at 2121.972 kb on + strand at 2122.108 kb on - strand at 2122.153 kb on + strand at 2122.219 kb on + strand, within RALBFv3_RS09525 at 2122.374 kb on + strand, within RALBFv3_RS09525 at 2122.389 kb on + strand, within RALBFv3_RS09525 at 2122.389 kb on + strand, within RALBFv3_RS09525 at 2122.390 kb on - strand, within RALBFv3_RS09525 at 2122.546 kb on + strand, within RALBFv3_RS09525 at 2122.547 kb on - strand, within RALBFv3_RS09525 at 2122.547 kb on - strand, within RALBFv3_RS09525 at 2122.628 kb on + strand at 2122.628 kb on + strand at 2122.629 kb on - strand at 2122.629 kb on - strand at 2122.762 kb on - strand at 2122.878 kb on + strand at 2122.878 kb on + strand at 2122.879 kb on - strand at 2123.025 kb on + strand, within RALBFv3_RS09530 at 2123.025 kb on + strand, within RALBFv3_RS09530 at 2123.025 kb on + strand, within RALBFv3_RS09530 at 2123.026 kb on - strand, within RALBFv3_RS09530 at 2123.026 kb on - strand, within RALBFv3_RS09530 at 2123.026 kb on - strand, within RALBFv3_RS09530 at 2123.026 kb on - strand, within RALBFv3_RS09530 at 2123.026 kb on - strand, within RALBFv3_RS09530 at 2123.026 kb on - strand, within RALBFv3_RS09530 at 2123.026 kb on - strand, within RALBFv3_RS09530 at 2123.034 kb on + strand, within RALBFv3_RS09530 at 2123.094 kb on + strand, within RALBFv3_RS09530 at 2123.316 kb on + strand, within RALBFv3_RS09530 at 2123.316 kb on + strand, within RALBFv3_RS09530 at 2123.317 kb on - strand, within RALBFv3_RS09530 at 2123.534 kb on + strand at 2123.535 kb on - strand at 2123.535 kb on - strand at 2123.535 kb on - strand at 2123.537 kb on + strand at 2123.537 kb on + strand at 2123.537 kb on + strand at 2123.538 kb on - strand at 2123.615 kb on + strand at 2123.616 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol remove 2,121,108 + +1.5 2,121,181 + +0.2 2,121,181 + -0.1 2,121,182 - -0.4 2,121,182 - +0.1 2,121,182 - +0.5 2,121,182 - -0.2 2,121,182 - -0.7 2,121,182 - -0.6 2,121,230 - +0.0 2,121,230 - -0.0 2,121,230 - +0.3 2,121,356 - RALBFv3_RS09520 0.23 +0.6 2,121,356 - RALBFv3_RS09520 0.23 -0.5 2,121,358 + RALBFv3_RS09520 0.23 -1.1 2,121,359 - RALBFv3_RS09520 0.24 +0.2 2,121,359 - RALBFv3_RS09520 0.24 -0.7 2,121,372 + RALBFv3_RS09520 0.25 +0.2 2,121,373 + RALBFv3_RS09520 0.25 +1.2 2,121,373 + RALBFv3_RS09520 0.25 +0.9 2,121,373 + RALBFv3_RS09520 0.25 -0.8 2,121,373 + RALBFv3_RS09520 0.25 +0.0 2,121,373 + RALBFv3_RS09520 0.25 -0.5 2,121,374 - RALBFv3_RS09520 0.26 -0.7 2,121,374 - RALBFv3_RS09520 0.26 +0.2 2,121,412 + RALBFv3_RS09520 0.31 -0.2 2,121,463 + RALBFv3_RS09520 0.37 +0.6 2,121,463 + RALBFv3_RS09520 0.37 -0.8 2,121,463 + RALBFv3_RS09520 0.37 +0.1 2,121,463 + RALBFv3_RS09520 0.37 +0.2 2,121,464 - RALBFv3_RS09520 0.37 -0.2 2,121,464 - RALBFv3_RS09520 0.37 +0.6 2,121,520 + RALBFv3_RS09520 0.45 +0.3 2,121,521 - RALBFv3_RS09520 0.45 +1.2 2,121,583 + RALBFv3_RS09520 0.53 -0.7 2,121,691 + RALBFv3_RS09520 0.67 -0.1 2,121,692 - RALBFv3_RS09520 0.67 -0.3 2,121,772 + RALBFv3_RS09520 0.78 +0.5 2,121,972 + -0.2 2,121,972 + -0.5 2,122,108 - -0.2 2,122,153 + -0.2 2,122,219 + RALBFv3_RS09525 0.22 -0.1 2,122,374 + RALBFv3_RS09525 0.51 -0.7 2,122,389 + RALBFv3_RS09525 0.54 -0.0 2,122,389 + RALBFv3_RS09525 0.54 -0.1 2,122,390 - RALBFv3_RS09525 0.54 +0.1 2,122,546 + RALBFv3_RS09525 0.84 +0.3 2,122,547 - RALBFv3_RS09525 0.84 +0.2 2,122,547 - RALBFv3_RS09525 0.84 -0.0 2,122,628 + -0.0 2,122,628 + +0.5 2,122,629 - +0.2 2,122,629 - +0.1 2,122,762 - -0.6 2,122,878 + +0.0 2,122,878 + -0.3 2,122,879 - -0.3 2,123,025 + RALBFv3_RS09530 0.26 +0.3 2,123,025 + RALBFv3_RS09530 0.26 +1.2 2,123,025 + RALBFv3_RS09530 0.26 -0.5 2,123,026 - RALBFv3_RS09530 0.26 +0.3 2,123,026 - RALBFv3_RS09530 0.26 +0.3 2,123,026 - RALBFv3_RS09530 0.26 +0.0 2,123,026 - RALBFv3_RS09530 0.26 +0.6 2,123,026 - RALBFv3_RS09530 0.26 -0.6 2,123,026 - RALBFv3_RS09530 0.26 -0.0 2,123,026 - RALBFv3_RS09530 0.26 -0.6 2,123,034 + RALBFv3_RS09530 0.27 +0.0 2,123,094 + RALBFv3_RS09530 0.36 +1.0 2,123,316 + RALBFv3_RS09530 0.71 -0.6 2,123,316 + RALBFv3_RS09530 0.71 -0.4 2,123,317 - RALBFv3_RS09530 0.71 +0.2 2,123,534 + -0.3 2,123,535 - -0.1 2,123,535 - +1.2 2,123,535 - +0.6 2,123,537 + +0.9 2,123,537 + +1.2 2,123,537 + -0.8 2,123,538 - -0.4 2,123,615 + -1.0 2,123,616 - -0.4
Or see this region's nucleotide sequence