Strain Fitness in Ralstonia solanacearum IBSBF1503 around RALBFv3_RS09525

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRALBFv3_RS09520 and RALBFv3_RS09525 are separated by 167 nucleotidesRALBFv3_RS09525 and RALBFv3_RS09530 are separated by 231 nucleotidesRALBFv3_RS09530 and RALBFv3_RS09535 overlap by 4 nucleotides RALBFv3_RS09520: RALBFv3_RS09520 - sel1 repeat family protein, at 2,121,180 to 2,121,938 _RS09520 RALBFv3_RS09525: RALBFv3_RS09525 - polyhydroxybutyrate granule-associated protein (phasin) phap2, at 2,122,106 to 2,122,627 _RS09525 RALBFv3_RS09530: RALBFv3_RS09530 - methionine biosynthesis protein MetW, at 2,122,859 to 2,123,506 _RS09530 RALBFv3_RS09535: RALBFv3_RS09535 - homoserine O-acetyltransferase, at 2,123,503 to 2,124,714 _RS09535 Position (kb) 2122 2123Strain fitness (log2 ratio) -1 0 1at 2121.108 kb on + strandat 2121.181 kb on + strandat 2121.181 kb on + strandat 2121.182 kb on - strandat 2121.182 kb on - strandat 2121.182 kb on - strandat 2121.182 kb on - strandat 2121.182 kb on - strandat 2121.182 kb on - strandat 2121.230 kb on - strandat 2121.230 kb on - strandat 2121.230 kb on - strandat 2121.356 kb on - strand, within RALBFv3_RS09520at 2121.356 kb on - strand, within RALBFv3_RS09520at 2121.358 kb on + strand, within RALBFv3_RS09520at 2121.359 kb on - strand, within RALBFv3_RS09520at 2121.359 kb on - strand, within RALBFv3_RS09520at 2121.372 kb on + strand, within RALBFv3_RS09520at 2121.373 kb on + strand, within RALBFv3_RS09520at 2121.373 kb on + strand, within RALBFv3_RS09520at 2121.373 kb on + strand, within RALBFv3_RS09520at 2121.373 kb on + strand, within RALBFv3_RS09520at 2121.373 kb on + strand, within RALBFv3_RS09520at 2121.374 kb on - strand, within RALBFv3_RS09520at 2121.374 kb on - strand, within RALBFv3_RS09520at 2121.412 kb on + strand, within RALBFv3_RS09520at 2121.463 kb on + strand, within RALBFv3_RS09520at 2121.463 kb on + strand, within RALBFv3_RS09520at 2121.463 kb on + strand, within RALBFv3_RS09520at 2121.463 kb on + strand, within RALBFv3_RS09520at 2121.464 kb on - strand, within RALBFv3_RS09520at 2121.464 kb on - strand, within RALBFv3_RS09520at 2121.520 kb on + strand, within RALBFv3_RS09520at 2121.521 kb on - strand, within RALBFv3_RS09520at 2121.583 kb on + strand, within RALBFv3_RS09520at 2121.691 kb on + strand, within RALBFv3_RS09520at 2121.692 kb on - strand, within RALBFv3_RS09520at 2121.772 kb on + strand, within RALBFv3_RS09520at 2121.972 kb on + strandat 2121.972 kb on + strandat 2122.108 kb on - strandat 2122.153 kb on + strandat 2122.219 kb on + strand, within RALBFv3_RS09525at 2122.374 kb on + strand, within RALBFv3_RS09525at 2122.389 kb on + strand, within RALBFv3_RS09525at 2122.389 kb on + strand, within RALBFv3_RS09525at 2122.390 kb on - strand, within RALBFv3_RS09525at 2122.546 kb on + strand, within RALBFv3_RS09525at 2122.547 kb on - strand, within RALBFv3_RS09525at 2122.547 kb on - strand, within RALBFv3_RS09525at 2122.628 kb on + strandat 2122.628 kb on + strandat 2122.629 kb on - strandat 2122.629 kb on - strandat 2122.762 kb on - strandat 2122.878 kb on + strandat 2122.878 kb on + strandat 2122.879 kb on - strandat 2123.025 kb on + strand, within RALBFv3_RS09530at 2123.025 kb on + strand, within RALBFv3_RS09530at 2123.025 kb on + strand, within RALBFv3_RS09530at 2123.026 kb on - strand, within RALBFv3_RS09530at 2123.026 kb on - strand, within RALBFv3_RS09530at 2123.026 kb on - strand, within RALBFv3_RS09530at 2123.026 kb on - strand, within RALBFv3_RS09530at 2123.026 kb on - strand, within RALBFv3_RS09530at 2123.026 kb on - strand, within RALBFv3_RS09530at 2123.026 kb on - strand, within RALBFv3_RS09530at 2123.034 kb on + strand, within RALBFv3_RS09530at 2123.094 kb on + strand, within RALBFv3_RS09530at 2123.316 kb on + strand, within RALBFv3_RS09530at 2123.316 kb on + strand, within RALBFv3_RS09530at 2123.317 kb on - strand, within RALBFv3_RS09530at 2123.534 kb on + strandat 2123.535 kb on - strandat 2123.535 kb on - strandat 2123.535 kb on - strandat 2123.537 kb on + strandat 2123.537 kb on + strandat 2123.537 kb on + strandat 2123.538 kb on - strandat 2123.615 kb on + strandat 2123.616 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol
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2,121,108 + +1.5
2,121,181 + +0.2
2,121,181 + -0.1
2,121,182 - -0.4
2,121,182 - +0.1
2,121,182 - +0.5
2,121,182 - -0.2
2,121,182 - -0.7
2,121,182 - -0.6
2,121,230 - +0.0
2,121,230 - -0.0
2,121,230 - +0.3
2,121,356 - RALBFv3_RS09520 0.23 +0.6
2,121,356 - RALBFv3_RS09520 0.23 -0.5
2,121,358 + RALBFv3_RS09520 0.23 -1.1
2,121,359 - RALBFv3_RS09520 0.24 +0.2
2,121,359 - RALBFv3_RS09520 0.24 -0.7
2,121,372 + RALBFv3_RS09520 0.25 +0.2
2,121,373 + RALBFv3_RS09520 0.25 +1.2
2,121,373 + RALBFv3_RS09520 0.25 +0.9
2,121,373 + RALBFv3_RS09520 0.25 -0.8
2,121,373 + RALBFv3_RS09520 0.25 +0.0
2,121,373 + RALBFv3_RS09520 0.25 -0.5
2,121,374 - RALBFv3_RS09520 0.26 -0.7
2,121,374 - RALBFv3_RS09520 0.26 +0.2
2,121,412 + RALBFv3_RS09520 0.31 -0.2
2,121,463 + RALBFv3_RS09520 0.37 +0.6
2,121,463 + RALBFv3_RS09520 0.37 -0.8
2,121,463 + RALBFv3_RS09520 0.37 +0.1
2,121,463 + RALBFv3_RS09520 0.37 +0.2
2,121,464 - RALBFv3_RS09520 0.37 -0.2
2,121,464 - RALBFv3_RS09520 0.37 +0.6
2,121,520 + RALBFv3_RS09520 0.45 +0.3
2,121,521 - RALBFv3_RS09520 0.45 +1.2
2,121,583 + RALBFv3_RS09520 0.53 -0.7
2,121,691 + RALBFv3_RS09520 0.67 -0.1
2,121,692 - RALBFv3_RS09520 0.67 -0.3
2,121,772 + RALBFv3_RS09520 0.78 +0.5
2,121,972 + -0.2
2,121,972 + -0.5
2,122,108 - -0.2
2,122,153 + -0.2
2,122,219 + RALBFv3_RS09525 0.22 -0.1
2,122,374 + RALBFv3_RS09525 0.51 -0.7
2,122,389 + RALBFv3_RS09525 0.54 -0.0
2,122,389 + RALBFv3_RS09525 0.54 -0.1
2,122,390 - RALBFv3_RS09525 0.54 +0.1
2,122,546 + RALBFv3_RS09525 0.84 +0.3
2,122,547 - RALBFv3_RS09525 0.84 +0.2
2,122,547 - RALBFv3_RS09525 0.84 -0.0
2,122,628 + -0.0
2,122,628 + +0.5
2,122,629 - +0.2
2,122,629 - +0.1
2,122,762 - -0.6
2,122,878 + +0.0
2,122,878 + -0.3
2,122,879 - -0.3
2,123,025 + RALBFv3_RS09530 0.26 +0.3
2,123,025 + RALBFv3_RS09530 0.26 +1.2
2,123,025 + RALBFv3_RS09530 0.26 -0.5
2,123,026 - RALBFv3_RS09530 0.26 +0.3
2,123,026 - RALBFv3_RS09530 0.26 +0.3
2,123,026 - RALBFv3_RS09530 0.26 +0.0
2,123,026 - RALBFv3_RS09530 0.26 +0.6
2,123,026 - RALBFv3_RS09530 0.26 -0.6
2,123,026 - RALBFv3_RS09530 0.26 -0.0
2,123,026 - RALBFv3_RS09530 0.26 -0.6
2,123,034 + RALBFv3_RS09530 0.27 +0.0
2,123,094 + RALBFv3_RS09530 0.36 +1.0
2,123,316 + RALBFv3_RS09530 0.71 -0.6
2,123,316 + RALBFv3_RS09530 0.71 -0.4
2,123,317 - RALBFv3_RS09530 0.71 +0.2
2,123,534 + -0.3
2,123,535 - -0.1
2,123,535 - +1.2
2,123,535 - +0.6
2,123,537 + +0.9
2,123,537 + +1.2
2,123,537 + -0.8
2,123,538 - -0.4
2,123,615 + -1.0
2,123,616 - -0.4

Or see this region's nucleotide sequence