Strain Fitness in Ralstonia solanacearum IBSBF1503 around RALBFv3_RS07260

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRALBFv3_RS07255 and RALBFv3_RS07260 are separated by 213 nucleotidesRALBFv3_RS07260 and RALBFv3_RS07265 are separated by 114 nucleotides RALBFv3_RS07255: RALBFv3_RS07255 - hypothetical protein, at 1,595,925 to 1,597,766 _RS07255 RALBFv3_RS07260: RALBFv3_RS07260 - DNA-directed RNA polymerase subunit beta', at 1,597,980 to 1,602,209 _RS07260 RALBFv3_RS07265: RALBFv3_RS07265 - DNA-directed RNA polymerase subunit beta, at 1,602,324 to 1,606,430 _RS07265 Position (kb) 1597 1598 1599 1600 1601 1602 1603Strain fitness (log2 ratio) -1 0 1at 1597.006 kb on + strand, within RALBFv3_RS07255at 1597.007 kb on - strand, within RALBFv3_RS07255at 1597.007 kb on - strand, within RALBFv3_RS07255at 1597.244 kb on - strand, within RALBFv3_RS07255at 1597.244 kb on - strand, within RALBFv3_RS07255at 1597.469 kb on - strand, within RALBFv3_RS07255at 1597.525 kb on + strand, within RALBFv3_RS07255at 1597.525 kb on + strand, within RALBFv3_RS07255at 1597.854 kb on + strandat 1597.982 kb on - strandat 1602.254 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol
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1,597,006 + RALBFv3_RS07255 0.59 +0.4
1,597,007 - RALBFv3_RS07255 0.59 +0.5
1,597,007 - RALBFv3_RS07255 0.59 +0.3
1,597,244 - RALBFv3_RS07255 0.72 +0.5
1,597,244 - RALBFv3_RS07255 0.72 +1.2
1,597,469 - RALBFv3_RS07255 0.84 +0.6
1,597,525 + RALBFv3_RS07255 0.87 +0.3
1,597,525 + RALBFv3_RS07255 0.87 +0.1
1,597,854 + -0.3
1,597,982 - +0.8
1,602,254 - +1.2

Or see this region's nucleotide sequence