Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS00240 and RALBFv3_RS00245 are separated by 34 nucleotides RALBFv3_RS00245 and RALBFv3_RS00250 are separated by 72 nucleotides
RALBFv3_RS00240: RALBFv3_RS00240 - glutathione ABC transporter ATP-binding protein, at 52,496 to 54,376
_RS00240
RALBFv3_RS00245: RALBFv3_RS00245 - glutathione ABC transporter substrate-binding protein GsiB, at 54,411 to 55,964
_RS00245
RALBFv3_RS00250: RALBFv3_RS00250 - glutathione ABC transporter permease GsiC, at 56,037 to 56,960
_RS00250
Position (kb) 54
55
56 Strain fitness (log2 ratio) -1
0
1 at 54.126 kb on - strand, within RALBFv3_RS00240 at 54.126 kb on - strand, within RALBFv3_RS00240 at 54.189 kb on - strand at 54.371 kb on + strand at 54.372 kb on - strand at 54.408 kb on + strand at 54.549 kb on + strand at 54.550 kb on - strand at 54.595 kb on - strand, within RALBFv3_RS00245 at 54.655 kb on - strand, within RALBFv3_RS00245 at 54.841 kb on - strand, within RALBFv3_RS00245 at 54.939 kb on + strand, within RALBFv3_RS00245 at 54.939 kb on + strand, within RALBFv3_RS00245 at 54.940 kb on - strand, within RALBFv3_RS00245 at 55.290 kb on + strand, within RALBFv3_RS00245 at 55.290 kb on + strand, within RALBFv3_RS00245 at 55.291 kb on - strand, within RALBFv3_RS00245 at 55.291 kb on - strand, within RALBFv3_RS00245 at 55.404 kb on + strand, within RALBFv3_RS00245 at 55.635 kb on + strand, within RALBFv3_RS00245 at 55.636 kb on - strand, within RALBFv3_RS00245 at 55.636 kb on - strand, within RALBFv3_RS00245 at 55.638 kb on + strand, within RALBFv3_RS00245 at 55.638 kb on + strand, within RALBFv3_RS00245 at 55.639 kb on - strand, within RALBFv3_RS00245 at 55.639 kb on - strand, within RALBFv3_RS00245 at 55.812 kb on + strand at 55.813 kb on - strand at 55.813 kb on - strand at 55.911 kb on + strand at 55.912 kb on - strand at 55.912 kb on - strand at 55.912 kb on - strand at 55.912 kb on - strand at 55.971 kb on - strand at 56.233 kb on + strand, within RALBFv3_RS00250 at 56.552 kb on + strand, within RALBFv3_RS00250 at 56.552 kb on + strand, within RALBFv3_RS00250 at 56.553 kb on + strand, within RALBFv3_RS00250 at 56.554 kb on - strand, within RALBFv3_RS00250 at 56.923 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol remove 54,126 - RALBFv3_RS00240 0.87 +1.0 54,126 - RALBFv3_RS00240 0.87 +0.0 54,189 - -0.7 54,371 + -0.0 54,372 - +1.1 54,408 + +0.3 54,549 + -0.3 54,550 - -0.9 54,595 - RALBFv3_RS00245 0.12 -0.4 54,655 - RALBFv3_RS00245 0.16 -0.0 54,841 - RALBFv3_RS00245 0.28 -0.3 54,939 + RALBFv3_RS00245 0.34 +1.0 54,939 + RALBFv3_RS00245 0.34 -0.8 54,940 - RALBFv3_RS00245 0.34 +0.3 55,290 + RALBFv3_RS00245 0.57 -0.1 55,290 + RALBFv3_RS00245 0.57 +0.6 55,291 - RALBFv3_RS00245 0.57 +0.7 55,291 - RALBFv3_RS00245 0.57 -0.4 55,404 + RALBFv3_RS00245 0.64 +0.0 55,635 + RALBFv3_RS00245 0.79 -0.1 55,636 - RALBFv3_RS00245 0.79 -1.0 55,636 - RALBFv3_RS00245 0.79 +0.5 55,638 + RALBFv3_RS00245 0.79 -0.3 55,638 + RALBFv3_RS00245 0.79 -0.4 55,639 - RALBFv3_RS00245 0.79 +0.4 55,639 - RALBFv3_RS00245 0.79 -1.6 55,812 + -0.1 55,813 - -0.3 55,813 - -1.3 55,911 + +0.1 55,912 - +0.2 55,912 - +0.3 55,912 - +1.1 55,912 - -0.4 55,971 - -0.3 56,233 + RALBFv3_RS00250 0.21 -0.6 56,552 + RALBFv3_RS00250 0.56 +0.0 56,552 + RALBFv3_RS00250 0.56 +1.0 56,553 + RALBFv3_RS00250 0.56 -0.2 56,554 - RALBFv3_RS00250 0.56 -0.6 56,923 - -0.6
Or see this region's nucleotide sequence