Strain Fitness in Ralstonia solanacearum IBSBF1503 around RALBFv3_RS00225

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRALBFv3_RS00220 and RALBFv3_RS00225 are separated by 31 nucleotidesRALBFv3_RS00225 and RALBFv3_RS00230 are separated by 79 nucleotides RALBFv3_RS00220: RALBFv3_RS00220 - CoA transferase, at 47,121 to 48,227 _RS00220 RALBFv3_RS00225: RALBFv3_RS00225 - glutathione ABC transporter ATP-binding protein, at 48,259 to 50,382 _RS00225 RALBFv3_RS00230: RALBFv3_RS00230 - MurR/RpiR family transcriptional regulator, at 50,462 to 51,469 _RS00230 Position (kb) 48 49 50 51Strain fitness (log2 ratio) -1 0 1at 47.761 kb on + strand, within RALBFv3_RS00220at 47.762 kb on - strand, within RALBFv3_RS00220at 49.532 kb on + strand, within RALBFv3_RS00225at 49.533 kb on - strand, within RALBFv3_RS00225at 49.533 kb on - strand, within RALBFv3_RS00225at 49.976 kb on + strand, within RALBFv3_RS00225at 50.166 kb on + strand, within RALBFv3_RS00225at 50.166 kb on + strand, within RALBFv3_RS00225at 50.201 kb on - strandat 50.201 kb on - strandat 50.264 kb on + strandat 50.429 kb on + strandat 50.593 kb on - strand, within RALBFv3_RS00230

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol
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47,761 + RALBFv3_RS00220 0.58 -0.1
47,762 - RALBFv3_RS00220 0.58 -0.5
49,532 + RALBFv3_RS00225 0.60 +0.7
49,533 - RALBFv3_RS00225 0.60 +1.0
49,533 - RALBFv3_RS00225 0.60 +1.0
49,976 + RALBFv3_RS00225 0.81 +0.7
50,166 + RALBFv3_RS00225 0.90 +0.3
50,166 + RALBFv3_RS00225 0.90 +0.6
50,201 - +0.5
50,201 - -0.7
50,264 + +0.0
50,429 + -1.6
50,593 - RALBFv3_RS00230 0.13 -0.6

Or see this region's nucleotide sequence