Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Bacitracin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS21455 and RALBFv3_RS21460 are separated by 96 nucleotides RALBFv3_RS21460 and RALBFv3_RS21465 are separated by 224 nucleotides RALBFv3_RS21465 and RALBFv3_RS24640 are separated by 29 nucleotides
RALBFv3_RS21455: RALBFv3_RS21455 - 2,3-diaminopropionate biosynthesis protein SbnA, at 1,536,537 to 1,537,553
_RS21455
RALBFv3_RS21460: RALBFv3_RS21460 - TonB-dependent siderophore receptor, at 1,537,650 to 1,540,076
_RS21460
RALBFv3_RS21465: RALBFv3_RS21465 - RNA polymerase sigma factor, at 1,540,301 to 1,540,891
_RS21465
RALBFv3_RS24640: RALBFv3_RS24640 - hypothetical protein, at 1,540,921 to 1,541,100
_RS24640
Position (kb)
1537
1538
1539
1540
1541 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1536.811 kb on + strand, within RALBFv3_RS21455 at 1536.812 kb on - strand, within RALBFv3_RS21455 at 1537.078 kb on + strand, within RALBFv3_RS21455 at 1537.078 kb on + strand, within RALBFv3_RS21455 at 1537.078 kb on + strand, within RALBFv3_RS21455 at 1537.079 kb on - strand, within RALBFv3_RS21455 at 1537.232 kb on - strand, within RALBFv3_RS21455 at 1537.232 kb on - strand, within RALBFv3_RS21455 at 1537.408 kb on + strand, within RALBFv3_RS21455 at 1537.409 kb on - strand, within RALBFv3_RS21455 at 1537.468 kb on - strand at 1537.489 kb on + strand at 1537.651 kb on + strand at 1537.652 kb on - strand at 1537.666 kb on + strand at 1537.666 kb on + strand at 1537.667 kb on - strand at 1537.667 kb on - strand at 1537.667 kb on - strand at 1537.712 kb on + strand at 1537.712 kb on + strand at 1537.739 kb on - strand at 1537.750 kb on + strand at 1537.810 kb on + strand at 1537.810 kb on + strand at 1537.810 kb on + strand at 1537.811 kb on - strand at 1537.811 kb on - strand at 1537.811 kb on - strand at 1537.838 kb on - strand at 1538.042 kb on - strand, within RALBFv3_RS21460 at 1538.186 kb on - strand, within RALBFv3_RS21460 at 1538.245 kb on + strand, within RALBFv3_RS21460 at 1538.245 kb on + strand, within RALBFv3_RS21460 at 1538.245 kb on + strand, within RALBFv3_RS21460 at 1538.246 kb on - strand, within RALBFv3_RS21460 at 1538.246 kb on - strand, within RALBFv3_RS21460 at 1538.246 kb on - strand, within RALBFv3_RS21460 at 1538.246 kb on - strand, within RALBFv3_RS21460 at 1538.359 kb on + strand, within RALBFv3_RS21460 at 1538.360 kb on - strand, within RALBFv3_RS21460 at 1538.360 kb on - strand, within RALBFv3_RS21460 at 1538.386 kb on + strand, within RALBFv3_RS21460 at 1538.386 kb on + strand, within RALBFv3_RS21460 at 1538.387 kb on - strand, within RALBFv3_RS21460 at 1538.387 kb on - strand, within RALBFv3_RS21460 at 1538.387 kb on - strand, within RALBFv3_RS21460 at 1538.446 kb on + strand, within RALBFv3_RS21460 at 1538.446 kb on + strand, within RALBFv3_RS21460 at 1538.447 kb on - strand, within RALBFv3_RS21460 at 1538.447 kb on - strand, within RALBFv3_RS21460 at 1538.605 kb on + strand, within RALBFv3_RS21460 at 1538.606 kb on - strand, within RALBFv3_RS21460 at 1538.629 kb on + strand, within RALBFv3_RS21460 at 1538.653 kb on + strand, within RALBFv3_RS21460 at 1538.875 kb on + strand, within RALBFv3_RS21460 at 1538.876 kb on - strand, within RALBFv3_RS21460 at 1538.876 kb on - strand, within RALBFv3_RS21460 at 1538.876 kb on - strand, within RALBFv3_RS21460 at 1538.921 kb on - strand, within RALBFv3_RS21460 at 1539.130 kb on + strand, within RALBFv3_RS21460 at 1539.130 kb on + strand, within RALBFv3_RS21460 at 1539.130 kb on + strand, within RALBFv3_RS21460 at 1539.130 kb on + strand, within RALBFv3_RS21460 at 1539.131 kb on - strand, within RALBFv3_RS21460 at 1539.131 kb on - strand, within RALBFv3_RS21460 at 1539.197 kb on - strand, within RALBFv3_RS21460 at 1539.198 kb on - strand, within RALBFv3_RS21460 at 1539.235 kb on + strand, within RALBFv3_RS21460 at 1539.265 kb on + strand, within RALBFv3_RS21460 at 1539.265 kb on + strand, within RALBFv3_RS21460 at 1539.265 kb on + strand, within RALBFv3_RS21460 at 1539.430 kb on - strand, within RALBFv3_RS21460 at 1540.005 kb on - strand at 1540.116 kb on - strand at 1540.156 kb on + strand at 1540.156 kb on + strand at 1540.156 kb on + strand at 1540.158 kb on - strand at 1540.204 kb on + strand at 1540.332 kb on + strand at 1540.429 kb on + strand, within RALBFv3_RS21465 at 1540.430 kb on - strand, within RALBFv3_RS21465 at 1540.825 kb on - strand, within RALBFv3_RS21465
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Bacitracin remove 1,536,811 + RALBFv3_RS21455 0.27 +0.7 1,536,812 - RALBFv3_RS21455 0.27 +0.5 1,537,078 + RALBFv3_RS21455 0.53 +0.5 1,537,078 + RALBFv3_RS21455 0.53 +0.3 1,537,078 + RALBFv3_RS21455 0.53 -0.1 1,537,079 - RALBFv3_RS21455 0.53 +0.6 1,537,232 - RALBFv3_RS21455 0.68 -0.4 1,537,232 - RALBFv3_RS21455 0.68 +0.1 1,537,408 + RALBFv3_RS21455 0.86 -0.7 1,537,409 - RALBFv3_RS21455 0.86 +0.7 1,537,468 - +0.1 1,537,489 + +0.4 1,537,651 + +0.7 1,537,652 - -0.5 1,537,666 + -2.0 1,537,666 + -2.1 1,537,667 - -1.8 1,537,667 - -0.9 1,537,667 - -0.3 1,537,712 + -1.5 1,537,712 + -0.6 1,537,739 - +0.5 1,537,750 + -0.6 1,537,810 + +0.7 1,537,810 + -0.5 1,537,810 + -1.7 1,537,811 - -0.9 1,537,811 - -0.2 1,537,811 - -3.4 1,537,838 - -0.3 1,538,042 - RALBFv3_RS21460 0.16 -0.3 1,538,186 - RALBFv3_RS21460 0.22 -1.4 1,538,245 + RALBFv3_RS21460 0.25 -2.5 1,538,245 + RALBFv3_RS21460 0.25 -2.7 1,538,245 + RALBFv3_RS21460 0.25 -1.1 1,538,246 - RALBFv3_RS21460 0.25 -0.5 1,538,246 - RALBFv3_RS21460 0.25 -0.3 1,538,246 - RALBFv3_RS21460 0.25 -0.0 1,538,246 - RALBFv3_RS21460 0.25 +0.0 1,538,359 + RALBFv3_RS21460 0.29 -0.6 1,538,360 - RALBFv3_RS21460 0.29 +0.7 1,538,360 - RALBFv3_RS21460 0.29 -0.7 1,538,386 + RALBFv3_RS21460 0.30 -1.9 1,538,386 + RALBFv3_RS21460 0.30 -1.6 1,538,387 - RALBFv3_RS21460 0.30 +0.0 1,538,387 - RALBFv3_RS21460 0.30 -0.6 1,538,387 - RALBFv3_RS21460 0.30 -0.3 1,538,446 + RALBFv3_RS21460 0.33 +0.1 1,538,446 + RALBFv3_RS21460 0.33 -1.6 1,538,447 - RALBFv3_RS21460 0.33 +1.7 1,538,447 - RALBFv3_RS21460 0.33 -0.8 1,538,605 + RALBFv3_RS21460 0.39 -0.7 1,538,606 - RALBFv3_RS21460 0.39 -0.6 1,538,629 + RALBFv3_RS21460 0.40 -0.6 1,538,653 + RALBFv3_RS21460 0.41 -3.0 1,538,875 + RALBFv3_RS21460 0.50 -0.3 1,538,876 - RALBFv3_RS21460 0.51 -2.5 1,538,876 - RALBFv3_RS21460 0.51 -1.3 1,538,876 - RALBFv3_RS21460 0.51 -0.3 1,538,921 - RALBFv3_RS21460 0.52 -0.3 1,539,130 + RALBFv3_RS21460 0.61 +0.7 1,539,130 + RALBFv3_RS21460 0.61 -1.2 1,539,130 + RALBFv3_RS21460 0.61 -1.1 1,539,130 + RALBFv3_RS21460 0.61 -0.9 1,539,131 - RALBFv3_RS21460 0.61 -0.4 1,539,131 - RALBFv3_RS21460 0.61 -0.2 1,539,197 - RALBFv3_RS21460 0.64 +0.5 1,539,198 - RALBFv3_RS21460 0.64 -0.5 1,539,235 + RALBFv3_RS21460 0.65 +0.7 1,539,265 + RALBFv3_RS21460 0.67 -2.4 1,539,265 + RALBFv3_RS21460 0.67 +0.7 1,539,265 + RALBFv3_RS21460 0.67 -2.9 1,539,430 - RALBFv3_RS21460 0.73 -0.6 1,540,005 - -0.2 1,540,116 - -1.6 1,540,156 + -1.8 1,540,156 + -1.4 1,540,156 + -1.3 1,540,158 - +0.4 1,540,204 + -0.9 1,540,332 + -0.4 1,540,429 + RALBFv3_RS21465 0.22 -0.3 1,540,430 - RALBFv3_RS21465 0.22 +0.7 1,540,825 - RALBFv3_RS21465 0.89 -0.7
Or see this region's nucleotide sequence