Strain Fitness in Ralstonia solanacearum IBSBF1503 around RALBFv3_RS21460

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Bacitracin

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRALBFv3_RS21455 and RALBFv3_RS21460 are separated by 96 nucleotidesRALBFv3_RS21460 and RALBFv3_RS21465 are separated by 224 nucleotidesRALBFv3_RS21465 and RALBFv3_RS24640 are separated by 29 nucleotides RALBFv3_RS21455: RALBFv3_RS21455 - 2,3-diaminopropionate biosynthesis protein SbnA, at 1,536,537 to 1,537,553 _RS21455 RALBFv3_RS21460: RALBFv3_RS21460 - TonB-dependent siderophore receptor, at 1,537,650 to 1,540,076 _RS21460 RALBFv3_RS21465: RALBFv3_RS21465 - RNA polymerase sigma factor, at 1,540,301 to 1,540,891 _RS21465 RALBFv3_RS24640: RALBFv3_RS24640 - hypothetical protein, at 1,540,921 to 1,541,100 _RS24640 Position (kb) 1537 1538 1539 1540 1541Strain fitness (log2 ratio) -3 -2 -1 0 1at 1536.811 kb on + strand, within RALBFv3_RS21455at 1536.812 kb on - strand, within RALBFv3_RS21455at 1537.078 kb on + strand, within RALBFv3_RS21455at 1537.078 kb on + strand, within RALBFv3_RS21455at 1537.078 kb on + strand, within RALBFv3_RS21455at 1537.079 kb on - strand, within RALBFv3_RS21455at 1537.232 kb on - strand, within RALBFv3_RS21455at 1537.232 kb on - strand, within RALBFv3_RS21455at 1537.408 kb on + strand, within RALBFv3_RS21455at 1537.409 kb on - strand, within RALBFv3_RS21455at 1537.468 kb on - strandat 1537.489 kb on + strandat 1537.651 kb on + strandat 1537.652 kb on - strandat 1537.666 kb on + strandat 1537.666 kb on + strandat 1537.667 kb on - strandat 1537.667 kb on - strandat 1537.667 kb on - strandat 1537.712 kb on + strandat 1537.712 kb on + strandat 1537.739 kb on - strandat 1537.750 kb on + strandat 1537.810 kb on + strandat 1537.810 kb on + strandat 1537.810 kb on + strandat 1537.811 kb on - strandat 1537.811 kb on - strandat 1537.811 kb on - strandat 1537.838 kb on - strandat 1538.042 kb on - strand, within RALBFv3_RS21460at 1538.186 kb on - strand, within RALBFv3_RS21460at 1538.245 kb on + strand, within RALBFv3_RS21460at 1538.245 kb on + strand, within RALBFv3_RS21460at 1538.245 kb on + strand, within RALBFv3_RS21460at 1538.246 kb on - strand, within RALBFv3_RS21460at 1538.246 kb on - strand, within RALBFv3_RS21460at 1538.246 kb on - strand, within RALBFv3_RS21460at 1538.246 kb on - strand, within RALBFv3_RS21460at 1538.359 kb on + strand, within RALBFv3_RS21460at 1538.360 kb on - strand, within RALBFv3_RS21460at 1538.360 kb on - strand, within RALBFv3_RS21460at 1538.386 kb on + strand, within RALBFv3_RS21460at 1538.386 kb on + strand, within RALBFv3_RS21460at 1538.387 kb on - strand, within RALBFv3_RS21460at 1538.387 kb on - strand, within RALBFv3_RS21460at 1538.387 kb on - strand, within RALBFv3_RS21460at 1538.446 kb on + strand, within RALBFv3_RS21460at 1538.446 kb on + strand, within RALBFv3_RS21460at 1538.447 kb on - strand, within RALBFv3_RS21460at 1538.447 kb on - strand, within RALBFv3_RS21460at 1538.605 kb on + strand, within RALBFv3_RS21460at 1538.606 kb on - strand, within RALBFv3_RS21460at 1538.629 kb on + strand, within RALBFv3_RS21460at 1538.653 kb on + strand, within RALBFv3_RS21460at 1538.875 kb on + strand, within RALBFv3_RS21460at 1538.876 kb on - strand, within RALBFv3_RS21460at 1538.876 kb on - strand, within RALBFv3_RS21460at 1538.876 kb on - strand, within RALBFv3_RS21460at 1538.921 kb on - strand, within RALBFv3_RS21460at 1539.130 kb on + strand, within RALBFv3_RS21460at 1539.130 kb on + strand, within RALBFv3_RS21460at 1539.130 kb on + strand, within RALBFv3_RS21460at 1539.130 kb on + strand, within RALBFv3_RS21460at 1539.131 kb on - strand, within RALBFv3_RS21460at 1539.131 kb on - strand, within RALBFv3_RS21460at 1539.197 kb on - strand, within RALBFv3_RS21460at 1539.198 kb on - strand, within RALBFv3_RS21460at 1539.235 kb on + strand, within RALBFv3_RS21460at 1539.265 kb on + strand, within RALBFv3_RS21460at 1539.265 kb on + strand, within RALBFv3_RS21460at 1539.265 kb on + strand, within RALBFv3_RS21460at 1539.430 kb on - strand, within RALBFv3_RS21460at 1540.005 kb on - strandat 1540.116 kb on - strandat 1540.156 kb on + strandat 1540.156 kb on + strandat 1540.156 kb on + strandat 1540.158 kb on - strandat 1540.204 kb on + strandat 1540.332 kb on + strandat 1540.429 kb on + strand, within RALBFv3_RS21465at 1540.430 kb on - strand, within RALBFv3_RS21465at 1540.825 kb on - strand, within RALBFv3_RS21465

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Bacitracin
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1,536,811 + RALBFv3_RS21455 0.27 +0.7
1,536,812 - RALBFv3_RS21455 0.27 +0.5
1,537,078 + RALBFv3_RS21455 0.53 +0.5
1,537,078 + RALBFv3_RS21455 0.53 +0.3
1,537,078 + RALBFv3_RS21455 0.53 -0.1
1,537,079 - RALBFv3_RS21455 0.53 +0.6
1,537,232 - RALBFv3_RS21455 0.68 -0.4
1,537,232 - RALBFv3_RS21455 0.68 +0.1
1,537,408 + RALBFv3_RS21455 0.86 -0.7
1,537,409 - RALBFv3_RS21455 0.86 +0.7
1,537,468 - +0.1
1,537,489 + +0.4
1,537,651 + +0.7
1,537,652 - -0.5
1,537,666 + -2.0
1,537,666 + -2.1
1,537,667 - -1.8
1,537,667 - -0.9
1,537,667 - -0.3
1,537,712 + -1.5
1,537,712 + -0.6
1,537,739 - +0.5
1,537,750 + -0.6
1,537,810 + +0.7
1,537,810 + -0.5
1,537,810 + -1.7
1,537,811 - -0.9
1,537,811 - -0.2
1,537,811 - -3.4
1,537,838 - -0.3
1,538,042 - RALBFv3_RS21460 0.16 -0.3
1,538,186 - RALBFv3_RS21460 0.22 -1.4
1,538,245 + RALBFv3_RS21460 0.25 -2.5
1,538,245 + RALBFv3_RS21460 0.25 -2.7
1,538,245 + RALBFv3_RS21460 0.25 -1.1
1,538,246 - RALBFv3_RS21460 0.25 -0.5
1,538,246 - RALBFv3_RS21460 0.25 -0.3
1,538,246 - RALBFv3_RS21460 0.25 -0.0
1,538,246 - RALBFv3_RS21460 0.25 +0.0
1,538,359 + RALBFv3_RS21460 0.29 -0.6
1,538,360 - RALBFv3_RS21460 0.29 +0.7
1,538,360 - RALBFv3_RS21460 0.29 -0.7
1,538,386 + RALBFv3_RS21460 0.30 -1.9
1,538,386 + RALBFv3_RS21460 0.30 -1.6
1,538,387 - RALBFv3_RS21460 0.30 +0.0
1,538,387 - RALBFv3_RS21460 0.30 -0.6
1,538,387 - RALBFv3_RS21460 0.30 -0.3
1,538,446 + RALBFv3_RS21460 0.33 +0.1
1,538,446 + RALBFv3_RS21460 0.33 -1.6
1,538,447 - RALBFv3_RS21460 0.33 +1.7
1,538,447 - RALBFv3_RS21460 0.33 -0.8
1,538,605 + RALBFv3_RS21460 0.39 -0.7
1,538,606 - RALBFv3_RS21460 0.39 -0.6
1,538,629 + RALBFv3_RS21460 0.40 -0.6
1,538,653 + RALBFv3_RS21460 0.41 -3.0
1,538,875 + RALBFv3_RS21460 0.50 -0.3
1,538,876 - RALBFv3_RS21460 0.51 -2.5
1,538,876 - RALBFv3_RS21460 0.51 -1.3
1,538,876 - RALBFv3_RS21460 0.51 -0.3
1,538,921 - RALBFv3_RS21460 0.52 -0.3
1,539,130 + RALBFv3_RS21460 0.61 +0.7
1,539,130 + RALBFv3_RS21460 0.61 -1.2
1,539,130 + RALBFv3_RS21460 0.61 -1.1
1,539,130 + RALBFv3_RS21460 0.61 -0.9
1,539,131 - RALBFv3_RS21460 0.61 -0.4
1,539,131 - RALBFv3_RS21460 0.61 -0.2
1,539,197 - RALBFv3_RS21460 0.64 +0.5
1,539,198 - RALBFv3_RS21460 0.64 -0.5
1,539,235 + RALBFv3_RS21460 0.65 +0.7
1,539,265 + RALBFv3_RS21460 0.67 -2.4
1,539,265 + RALBFv3_RS21460 0.67 +0.7
1,539,265 + RALBFv3_RS21460 0.67 -2.9
1,539,430 - RALBFv3_RS21460 0.73 -0.6
1,540,005 - -0.2
1,540,116 - -1.6
1,540,156 + -1.8
1,540,156 + -1.4
1,540,156 + -1.3
1,540,158 - +0.4
1,540,204 + -0.9
1,540,332 + -0.4
1,540,429 + RALBFv3_RS21465 0.22 -0.3
1,540,430 - RALBFv3_RS21465 0.22 +0.7
1,540,825 - RALBFv3_RS21465 0.89 -0.7

Or see this region's nucleotide sequence