Strain Fitness in Ralstonia solanacearum IBSBF1503 around RALBFv3_RS19555

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Bacitracin

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRALBFv3_RS19545 and RALBFv3_RS19550 are separated by 20 nucleotidesRALBFv3_RS19550 and RALBFv3_RS19555 are separated by 282 nucleotidesRALBFv3_RS19555 and RALBFv3_RS19560 are separated by 18 nucleotides RALBFv3_RS19545: RALBFv3_RS19545 - type III secretion protein HrpB2, at 1,060,379 to 1,060,765 _RS19545 RALBFv3_RS19550: RALBFv3_RS19550 - HrpB1 family type III secretion system apparatus protein, at 1,060,786 to 1,061,271 _RS19550 RALBFv3_RS19555: RALBFv3_RS19555 - EscU/YscU/HrcU family type III secretion system export apparatus switch protein, at 1,061,554 to 1,062,618 _RS19555 RALBFv3_RS19560: RALBFv3_RS19560 - EscV/YscV/HrcV family type III secretion system export apparatus protein, at 1,062,637 to 1,064,709 _RS19560 Position (kb) 1061 1062 1063Strain fitness (log2 ratio) -1 0 1 2at 1060.803 kb on - strandat 1061.214 kb on - strand, within RALBFv3_RS19550at 1061.214 kb on - strand, within RALBFv3_RS19550at 1061.214 kb on - strand, within RALBFv3_RS19550at 1061.214 kb on - strand, within RALBFv3_RS19550at 1061.347 kb on + strandat 1061.348 kb on - strandat 1062.391 kb on + strand, within RALBFv3_RS19555at 1062.392 kb on - strand, within RALBFv3_RS19555at 1062.392 kb on - strand, within RALBFv3_RS19555at 1062.803 kb on - strandat 1063.285 kb on + strand, within RALBFv3_RS19560at 1063.588 kb on + strand, within RALBFv3_RS19560

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Bacitracin
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1,060,803 - -0.1
1,061,214 - RALBFv3_RS19550 0.88 +0.2
1,061,214 - RALBFv3_RS19550 0.88 +0.3
1,061,214 - RALBFv3_RS19550 0.88 +2.2
1,061,214 - RALBFv3_RS19550 0.88 -0.8
1,061,347 + +1.0
1,061,348 - +0.3
1,062,391 + RALBFv3_RS19555 0.79 -0.1
1,062,392 - RALBFv3_RS19555 0.79 +0.6
1,062,392 - RALBFv3_RS19555 0.79 +0.5
1,062,803 - -0.4
1,063,285 + RALBFv3_RS19560 0.31 +0.1
1,063,588 + RALBFv3_RS19560 0.46 -1.0

Or see this region's nucleotide sequence