Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Bacitracin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS19545 and RALBFv3_RS19550 are separated by 20 nucleotides RALBFv3_RS19550 and RALBFv3_RS19555 are separated by 282 nucleotides RALBFv3_RS19555 and RALBFv3_RS19560 are separated by 18 nucleotides
RALBFv3_RS19545: RALBFv3_RS19545 - type III secretion protein HrpB2, at 1,060,379 to 1,060,765
_RS19545
RALBFv3_RS19550: RALBFv3_RS19550 - HrpB1 family type III secretion system apparatus protein, at 1,060,786 to 1,061,271
_RS19550
RALBFv3_RS19555: RALBFv3_RS19555 - EscU/YscU/HrcU family type III secretion system export apparatus switch protein, at 1,061,554 to 1,062,618
_RS19555
RALBFv3_RS19560: RALBFv3_RS19560 - EscV/YscV/HrcV family type III secretion system export apparatus protein, at 1,062,637 to 1,064,709
_RS19560
Position (kb)
1061
1062
1063 Strain fitness (log2 ratio)
-1
0
1
2 at 1060.803 kb on - strand at 1061.214 kb on - strand, within RALBFv3_RS19550 at 1061.214 kb on - strand, within RALBFv3_RS19550 at 1061.214 kb on - strand, within RALBFv3_RS19550 at 1061.214 kb on - strand, within RALBFv3_RS19550 at 1061.347 kb on + strand at 1061.348 kb on - strand at 1062.391 kb on + strand, within RALBFv3_RS19555 at 1062.392 kb on - strand, within RALBFv3_RS19555 at 1062.392 kb on - strand, within RALBFv3_RS19555 at 1062.803 kb on - strand at 1063.285 kb on + strand, within RALBFv3_RS19560 at 1063.588 kb on + strand, within RALBFv3_RS19560
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Bacitracin remove 1,060,803 - -0.1 1,061,214 - RALBFv3_RS19550 0.88 +0.2 1,061,214 - RALBFv3_RS19550 0.88 +0.3 1,061,214 - RALBFv3_RS19550 0.88 +2.2 1,061,214 - RALBFv3_RS19550 0.88 -0.8 1,061,347 + +1.0 1,061,348 - +0.3 1,062,391 + RALBFv3_RS19555 0.79 -0.1 1,062,392 - RALBFv3_RS19555 0.79 +0.6 1,062,392 - RALBFv3_RS19555 0.79 +0.5 1,062,803 - -0.4 1,063,285 + RALBFv3_RS19560 0.31 +0.1 1,063,588 + RALBFv3_RS19560 0.46 -1.0
Or see this region's nucleotide sequence