Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Bacitracin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS04785 and RALBFv3_RS04790 are separated by 30 nucleotides RALBFv3_RS04790 and RALBFv3_RS04795 are separated by 156 nucleotides RALBFv3_RS04795 and RALBFv3_RS04800 overlap by 4 nucleotides
RALBFv3_RS04785: RALBFv3_RS04785 - lipoprotein signal peptidase, at 1,070,235 to 1,070,759
_RS04785
RALBFv3_RS04790: RALBFv3_RS04790 - YdcF family protein, at 1,070,790 to 1,071,362
_RS04790
RALBFv3_RS04795: RALBFv3_RS04795 - bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC, at 1,071,519 to 1,072,724
_RS04795
RALBFv3_RS04800: RALBFv3_RS04800 - LLM class flavin-dependent oxidoreductase, at 1,072,721 to 1,073,773
_RS04800
Position (kb)
1071
1072
1073 Strain fitness (log2 ratio)
-2
-1
0
1 at 1070.806 kb on + strand at 1070.851 kb on + strand, within RALBFv3_RS04790 at 1070.852 kb on - strand, within RALBFv3_RS04790 at 1070.876 kb on + strand, within RALBFv3_RS04790 at 1071.163 kb on + strand, within RALBFv3_RS04790 at 1071.163 kb on + strand, within RALBFv3_RS04790 at 1071.464 kb on + strand at 1072.847 kb on - strand, within RALBFv3_RS04800 at 1072.847 kb on - strand, within RALBFv3_RS04800 at 1072.847 kb on - strand, within RALBFv3_RS04800 at 1072.853 kb on + strand, within RALBFv3_RS04800 at 1072.854 kb on - strand, within RALBFv3_RS04800 at 1072.854 kb on - strand, within RALBFv3_RS04800 at 1072.927 kb on + strand, within RALBFv3_RS04800 at 1072.976 kb on + strand, within RALBFv3_RS04800 at 1072.976 kb on + strand, within RALBFv3_RS04800 at 1072.979 kb on + strand, within RALBFv3_RS04800 at 1072.980 kb on - strand, within RALBFv3_RS04800 at 1072.980 kb on - strand, within RALBFv3_RS04800 at 1072.980 kb on - strand, within RALBFv3_RS04800 at 1073.109 kb on - strand, within RALBFv3_RS04800 at 1073.349 kb on - strand, within RALBFv3_RS04800 at 1073.387 kb on + strand, within RALBFv3_RS04800 at 1073.388 kb on - strand, within RALBFv3_RS04800 at 1073.536 kb on + strand, within RALBFv3_RS04800 at 1073.700 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Bacitracin remove 1,070,806 + +0.2 1,070,851 + RALBFv3_RS04790 0.11 +0.1 1,070,852 - RALBFv3_RS04790 0.11 -1.2 1,070,876 + RALBFv3_RS04790 0.15 -1.1 1,071,163 + RALBFv3_RS04790 0.65 -0.2 1,071,163 + RALBFv3_RS04790 0.65 -0.3 1,071,464 + +0.4 1,072,847 - RALBFv3_RS04800 0.12 -0.6 1,072,847 - RALBFv3_RS04800 0.12 -0.0 1,072,847 - RALBFv3_RS04800 0.12 +1.0 1,072,853 + RALBFv3_RS04800 0.13 -0.2 1,072,854 - RALBFv3_RS04800 0.13 +0.1 1,072,854 - RALBFv3_RS04800 0.13 -0.4 1,072,927 + RALBFv3_RS04800 0.20 +0.5 1,072,976 + RALBFv3_RS04800 0.24 -0.1 1,072,976 + RALBFv3_RS04800 0.24 +1.0 1,072,979 + RALBFv3_RS04800 0.25 +0.4 1,072,980 - RALBFv3_RS04800 0.25 -2.6 1,072,980 - RALBFv3_RS04800 0.25 +0.7 1,072,980 - RALBFv3_RS04800 0.25 +0.2 1,073,109 - RALBFv3_RS04800 0.37 -1.9 1,073,349 - RALBFv3_RS04800 0.60 -0.0 1,073,387 + RALBFv3_RS04800 0.63 +0.2 1,073,388 - RALBFv3_RS04800 0.63 -0.3 1,073,536 + RALBFv3_RS04800 0.77 +1.1 1,073,700 - -0.5
Or see this region's nucleotide sequence