Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Bacitracin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS03120 and RALBFv3_RS03125 are separated by 56 nucleotides RALBFv3_RS03125 and RALBFv3_RS03130 are separated by 142 nucleotides RALBFv3_RS03130 and RALBFv3_RS03135 are separated by 20 nucleotides
RALBFv3_RS03120: RALBFv3_RS03120 - ABC transporter permease, at 681,228 to 683,759
_RS03120
RALBFv3_RS03125: RALBFv3_RS03125 - hypothetical protein, at 683,816 to 684,916
_RS03125
RALBFv3_RS03130: RALBFv3_RS03130 - tungsten ABC transporter substrate-binding protein, at 685,059 to 685,895
_RS03130
RALBFv3_RS03135: RALBFv3_RS03135 - LysR family transcriptional regulator, at 685,916 to 686,860
_RS03135
Position (kb) 683
684
685 Strain fitness (log2 ratio) -1
0
1 at 682.869 kb on - strand, within RALBFv3_RS03120 at 684.007 kb on - strand, within RALBFv3_RS03125 at 684.007 kb on - strand, within RALBFv3_RS03125 at 684.009 kb on + strand, within RALBFv3_RS03125 at 684.010 kb on - strand, within RALBFv3_RS03125 at 684.010 kb on - strand, within RALBFv3_RS03125 at 684.010 kb on - strand, within RALBFv3_RS03125 at 684.010 kb on - strand, within RALBFv3_RS03125 at 684.404 kb on + strand, within RALBFv3_RS03125 at 684.848 kb on + strand at 684.848 kb on + strand at 684.848 kb on + strand at 684.848 kb on + strand at 684.849 kb on - strand at 684.849 kb on - strand at 684.939 kb on + strand at 684.940 kb on - strand at 684.978 kb on + strand at 685.026 kb on - strand at 685.026 kb on - strand at 685.026 kb on - strand at 685.089 kb on + strand at 685.089 kb on + strand at 685.089 kb on + strand at 685.090 kb on - strand at 685.171 kb on - strand, within RALBFv3_RS03130 at 685.371 kb on + strand, within RALBFv3_RS03130 at 685.371 kb on + strand, within RALBFv3_RS03130 at 685.372 kb on - strand, within RALBFv3_RS03130 at 685.372 kb on - strand, within RALBFv3_RS03130 at 685.372 kb on - strand, within RALBFv3_RS03130 at 685.556 kb on + strand, within RALBFv3_RS03130 at 685.567 kb on - strand, within RALBFv3_RS03130 at 685.626 kb on + strand, within RALBFv3_RS03130 at 685.627 kb on - strand, within RALBFv3_RS03130 at 685.652 kb on - strand, within RALBFv3_RS03130 at 685.764 kb on + strand, within RALBFv3_RS03130 at 685.764 kb on + strand, within RALBFv3_RS03130 at 685.764 kb on + strand, within RALBFv3_RS03130 at 685.765 kb on - strand, within RALBFv3_RS03130 at 685.765 kb on - strand, within RALBFv3_RS03130 at 685.866 kb on + strand at 685.893 kb on + strand at 685.894 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Bacitracin remove 682,869 - RALBFv3_RS03120 0.65 -0.5 684,007 - RALBFv3_RS03125 0.17 +0.4 684,007 - RALBFv3_RS03125 0.17 +0.2 684,009 + RALBFv3_RS03125 0.18 -0.0 684,010 - RALBFv3_RS03125 0.18 -0.1 684,010 - RALBFv3_RS03125 0.18 +0.3 684,010 - RALBFv3_RS03125 0.18 -0.3 684,010 - RALBFv3_RS03125 0.18 -0.1 684,404 + RALBFv3_RS03125 0.53 +1.2 684,848 + -0.9 684,848 + +0.6 684,848 + -0.5 684,848 + +0.9 684,849 - -0.3 684,849 - -0.8 684,939 + -0.4 684,940 - +0.7 684,978 + -0.6 685,026 - +0.3 685,026 - +0.4 685,026 - +0.7 685,089 + -0.5 685,089 + -0.8 685,089 + +0.5 685,090 - -0.6 685,171 - RALBFv3_RS03130 0.13 +1.2 685,371 + RALBFv3_RS03130 0.37 +0.9 685,371 + RALBFv3_RS03130 0.37 +0.1 685,372 - RALBFv3_RS03130 0.37 +0.0 685,372 - RALBFv3_RS03130 0.37 -0.4 685,372 - RALBFv3_RS03130 0.37 +0.1 685,556 + RALBFv3_RS03130 0.59 -0.1 685,567 - RALBFv3_RS03130 0.61 +0.3 685,626 + RALBFv3_RS03130 0.68 -0.6 685,627 - RALBFv3_RS03130 0.68 -0.4 685,652 - RALBFv3_RS03130 0.71 +0.3 685,764 + RALBFv3_RS03130 0.84 +0.0 685,764 + RALBFv3_RS03130 0.84 -0.7 685,764 + RALBFv3_RS03130 0.84 +0.0 685,765 - RALBFv3_RS03130 0.84 +0.1 685,765 - RALBFv3_RS03130 0.84 +1.3 685,866 + -0.2 685,893 + -0.1 685,894 - +1.5
Or see this region's nucleotide sequence