Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS20735 and RALBFv3_RS20745 are separated by 455 nucleotides RALBFv3_RS20745 and RALBFv3_RS20750 are separated by 198 nucleotides
RALBFv3_RS20735: RALBFv3_RS20735 - MFS transporter, at 1,348,474 to 1,349,610
_RS20735
RALBFv3_RS20745: RALBFv3_RS20745 - IS3 family transposase, at 1,350,066 to 1,351,189
_RS20745
RALBFv3_RS20750: RALBFv3_RS20750 - IS3 family transposase, at 1,351,388 to 1,352,622
_RS20750
Position (kb)
1350
1351
1352 Strain fitness (log2 ratio)
-1
0
1 at 1349.099 kb on + strand, within RALBFv3_RS20735 at 1349.099 kb on + strand, within RALBFv3_RS20735 at 1349.099 kb on + strand, within RALBFv3_RS20735 at 1349.100 kb on - strand, within RALBFv3_RS20735 at 1349.100 kb on - strand, within RALBFv3_RS20735 at 1349.100 kb on - strand, within RALBFv3_RS20735 at 1349.100 kb on - strand, within RALBFv3_RS20735 at 1349.122 kb on + strand, within RALBFv3_RS20735 at 1349.122 kb on + strand, within RALBFv3_RS20735 at 1349.123 kb on - strand, within RALBFv3_RS20735 at 1349.123 kb on - strand, within RALBFv3_RS20735 at 1349.535 kb on + strand at 1349.785 kb on + strand at 1349.786 kb on + strand at 1349.786 kb on + strand at 1349.787 kb on - strand at 1349.787 kb on - strand at 1349.787 kb on - strand at 1349.787 kb on - strand at 1349.787 kb on - strand at 1349.787 kb on - strand at 1349.852 kb on + strand at 1349.853 kb on - strand at 1350.027 kb on - strand at 1350.027 kb on - strand at 1350.027 kb on - strand at 1350.040 kb on - strand at 1350.040 kb on - strand at 1350.040 kb on - strand at 1350.040 kb on - strand at 1351.210 kb on + strand at 1351.210 kb on + strand at 1351.210 kb on + strand at 1351.211 kb on - strand at 1351.323 kb on + strand at 1351.323 kb on + strand at 1351.323 kb on + strand at 1351.324 kb on - strand at 1351.324 kb on - strand at 1351.336 kb on - strand at 1351.347 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin remove 1,349,099 + RALBFv3_RS20735 0.55 +0.3 1,349,099 + RALBFv3_RS20735 0.55 +0.1 1,349,099 + RALBFv3_RS20735 0.55 -0.1 1,349,100 - RALBFv3_RS20735 0.55 -0.0 1,349,100 - RALBFv3_RS20735 0.55 -0.3 1,349,100 - RALBFv3_RS20735 0.55 +0.2 1,349,100 - RALBFv3_RS20735 0.55 -0.3 1,349,122 + RALBFv3_RS20735 0.57 -0.4 1,349,122 + RALBFv3_RS20735 0.57 -0.0 1,349,123 - RALBFv3_RS20735 0.57 +0.0 1,349,123 - RALBFv3_RS20735 0.57 +0.7 1,349,535 + -0.0 1,349,785 + +0.4 1,349,786 + +0.7 1,349,786 + -0.5 1,349,787 - +0.8 1,349,787 - -0.3 1,349,787 - -0.3 1,349,787 - +0.1 1,349,787 - -0.4 1,349,787 - +0.8 1,349,852 + -0.2 1,349,853 - -0.3 1,350,027 - -0.4 1,350,027 - +0.6 1,350,027 - +0.8 1,350,040 - +0.2 1,350,040 - +0.1 1,350,040 - -0.7 1,350,040 - +0.2 1,351,210 + +0.8 1,351,210 + +0.2 1,351,210 + +0.2 1,351,211 - -1.0 1,351,323 + +0.8 1,351,323 + -0.7 1,351,323 + -0.1 1,351,324 - +0.0 1,351,324 - -0.2 1,351,336 - -0.5 1,351,347 + -0.8
Or see this region's nucleotide sequence