Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS02645 and RALBFv3_RS02650 are separated by 10 nucleotides RALBFv3_RS02650 and RALBFv3_RS02655 are separated by 253 nucleotides RALBFv3_RS02655 and RALBFv3_RS02660 are separated by 355 nucleotides
RALBFv3_RS02645: RALBFv3_RS02645 - succinate dehydrogenase, hydrophobic membrane anchor protein, at 589,713 to 590,078
_RS02645
RALBFv3_RS02650: RALBFv3_RS02650 - succinate dehydrogenase, cytochrome b556 subunit, at 590,089 to 590,499
_RS02650
RALBFv3_RS02655: RALBFv3_RS02655 - GntR family transcriptional regulator, at 590,753 to 591,559
_RS02655
RALBFv3_RS02660: RALBFv3_RS02660 - malate dehydrogenase, at 591,915 to 592,904
_RS02660
Position (kb) 590
591
592 Strain fitness (log2 ratio) -1
0
1 at 590.773 kb on - strand at 590.776 kb on - strand at 590.776 kb on - strand at 590.794 kb on - strand at 590.976 kb on + strand, within RALBFv3_RS02655 at 590.977 kb on - strand, within RALBFv3_RS02655 at 590.977 kb on - strand, within RALBFv3_RS02655 at 591.249 kb on + strand, within RALBFv3_RS02655 at 591.668 kb on - strand at 591.668 kb on - strand at 591.765 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin remove 590,773 - -0.0 590,776 - -0.6 590,776 - +0.3 590,794 - +0.2 590,976 + RALBFv3_RS02655 0.28 -1.1 590,977 - RALBFv3_RS02655 0.28 +1.0 590,977 - RALBFv3_RS02655 0.28 -1.6 591,249 + RALBFv3_RS02655 0.61 +0.5 591,668 - -0.6 591,668 - -0.5 591,765 + -0.2
Or see this region's nucleotide sequence