Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS01740 and RALBFv3_RS01745 overlap by 9 nucleotides RALBFv3_RS01745 and RALBFv3_RS01750 are separated by 116 nucleotides RALBFv3_RS01750 and RALBFv3_RS01755 are separated by 181 nucleotides RALBFv3_RS01755 and RALBFv3_RS01760 are separated by 58 nucleotides RALBFv3_RS01760 and RALBFv3_RS01765 are separated by 24 nucleotides
RALBFv3_RS01740: RALBFv3_RS01740 - aldose epimerase, at 391,973 to 392,926
_RS01740
RALBFv3_RS01745: RALBFv3_RS01745 - carboxymuconolactone decarboxylase family protein, at 392,918 to 393,277
_RS01745
RALBFv3_RS01750: RALBFv3_RS01750 - AraC family transcriptional regulator, at 393,394 to 394,314
_RS01750
RALBFv3_RS01755: RALBFv3_RS01755 - tRNA-Pro, at 394,496 to 394,572
_RS01755
RALBFv3_RS01760: RALBFv3_RS01760 - MerR family transcriptional regulator, at 394,631 to 395,065
_RS01760
RALBFv3_RS01765: RALBFv3_RS01765 - integration host factor subunit alpha, at 395,090 to 395,512
_RS01765
Position (kb) 393
394
395 Strain fitness (log2 ratio) -1
0
1
2 at 392.452 kb on + strand, within RALBFv3_RS01740 at 392.493 kb on - strand, within RALBFv3_RS01740 at 392.527 kb on + strand, within RALBFv3_RS01740 at 392.528 kb on - strand, within RALBFv3_RS01740 at 392.585 kb on - strand, within RALBFv3_RS01740 at 392.603 kb on - strand, within RALBFv3_RS01740 at 392.611 kb on + strand, within RALBFv3_RS01740 at 392.795 kb on + strand, within RALBFv3_RS01740 at 392.795 kb on + strand, within RALBFv3_RS01740 at 392.796 kb on - strand, within RALBFv3_RS01740 at 392.899 kb on + strand at 392.899 kb on + strand at 392.982 kb on + strand, within RALBFv3_RS01745 at 393.088 kb on + strand, within RALBFv3_RS01745 at 393.292 kb on - strand at 393.331 kb on + strand at 393.331 kb on + strand at 394.096 kb on + strand, within RALBFv3_RS01750 at 394.189 kb on - strand, within RALBFv3_RS01750 at 394.552 kb on - strand, within RALBFv3_RS01755 at 394.587 kb on + strand at 394.588 kb on - strand at 394.891 kb on - strand, within RALBFv3_RS01760 at 394.894 kb on - strand, within RALBFv3_RS01760 at 395.047 kb on + strand at 395.047 kb on + strand at 395.048 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin remove 392,452 + RALBFv3_RS01740 0.50 +0.0 392,493 - RALBFv3_RS01740 0.55 -0.9 392,527 + RALBFv3_RS01740 0.58 +1.4 392,528 - RALBFv3_RS01740 0.58 +2.5 392,585 - RALBFv3_RS01740 0.64 +0.2 392,603 - RALBFv3_RS01740 0.66 -0.1 392,611 + RALBFv3_RS01740 0.67 -0.5 392,795 + RALBFv3_RS01740 0.86 -0.4 392,795 + RALBFv3_RS01740 0.86 +0.2 392,796 - RALBFv3_RS01740 0.86 -0.2 392,899 + -0.6 392,899 + +0.2 392,982 + RALBFv3_RS01745 0.18 -0.0 393,088 + RALBFv3_RS01745 0.47 +0.2 393,292 - -0.0 393,331 + -0.1 393,331 + -0.0 394,096 + RALBFv3_RS01750 0.76 -0.2 394,189 - RALBFv3_RS01750 0.86 +0.4 394,552 - RALBFv3_RS01755 0.73 -0.8 394,587 + -0.4 394,588 - -0.0 394,891 - RALBFv3_RS01760 0.60 -0.4 394,894 - RALBFv3_RS01760 0.60 +0.2 395,047 + -0.0 395,047 + -0.8 395,048 - +0.7
Or see this region's nucleotide sequence