Experiment: SMSA with Ralstonia_antibiotic_mix
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS21315 and RALBFv3_RS21320 are separated by 41 nucleotides RALBFv3_RS21320 and RALBFv3_RS21325 overlap by 4 nucleotides RALBFv3_RS21325 and RALBFv3_RS21330 are separated by 137 nucleotides RALBFv3_RS21330 and RALBFv3_RS21335 are separated by 266 nucleotides
RALBFv3_RS21315: RALBFv3_RS21315 - hydrolase, at 1,504,015 to 1,504,632
_RS21315
RALBFv3_RS21320: RALBFv3_RS21320 - amidinotransferase, at 1,504,674 to 1,505,633
_RS21320
RALBFv3_RS21325: RALBFv3_RS21325 - ornithine cyclodeaminase, at 1,505,630 to 1,506,679
_RS21325
RALBFv3_RS21330: RALBFv3_RS21330 - Lrp/AsnC family transcriptional regulator, at 1,506,817 to 1,507,254
_RS21330
RALBFv3_RS21335: RALBFv3_RS21335 - amino acid ABC transporter substrate-binding protein, at 1,507,521 to 1,508,285
_RS21335
Position (kb)
1505
1506
1507 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1504.630 kb on + strand at 1505.017 kb on - strand, within RALBFv3_RS21320 at 1505.017 kb on - strand, within RALBFv3_RS21320 at 1505.017 kb on - strand, within RALBFv3_RS21320 at 1505.017 kb on - strand, within RALBFv3_RS21320 at 1505.017 kb on - strand, within RALBFv3_RS21320 at 1505.017 kb on - strand, within RALBFv3_RS21320 at 1505.029 kb on + strand, within RALBFv3_RS21320 at 1505.030 kb on - strand, within RALBFv3_RS21320 at 1505.030 kb on - strand, within RALBFv3_RS21320 at 1505.030 kb on - strand, within RALBFv3_RS21320 at 1505.140 kb on + strand, within RALBFv3_RS21320 at 1505.141 kb on - strand, within RALBFv3_RS21320 at 1505.218 kb on + strand, within RALBFv3_RS21320 at 1505.303 kb on - strand, within RALBFv3_RS21320 at 1505.303 kb on - strand, within RALBFv3_RS21320 at 1505.311 kb on + strand, within RALBFv3_RS21320 at 1505.312 kb on - strand, within RALBFv3_RS21320 at 1505.313 kb on + strand, within RALBFv3_RS21320 at 1505.314 kb on - strand, within RALBFv3_RS21320 at 1505.458 kb on + strand, within RALBFv3_RS21320 at 1505.459 kb on - strand, within RALBFv3_RS21320 at 1505.763 kb on + strand, within RALBFv3_RS21325 at 1505.763 kb on + strand, within RALBFv3_RS21325 at 1505.763 kb on + strand, within RALBFv3_RS21325 at 1505.764 kb on - strand, within RALBFv3_RS21325 at 1505.764 kb on - strand, within RALBFv3_RS21325 at 1505.765 kb on - strand, within RALBFv3_RS21325 at 1505.778 kb on + strand, within RALBFv3_RS21325 at 1505.778 kb on + strand, within RALBFv3_RS21325 at 1505.779 kb on - strand, within RALBFv3_RS21325 at 1505.779 kb on - strand, within RALBFv3_RS21325 at 1506.060 kb on + strand, within RALBFv3_RS21325 at 1506.061 kb on - strand, within RALBFv3_RS21325 at 1506.061 kb on - strand, within RALBFv3_RS21325 at 1506.164 kb on - strand, within RALBFv3_RS21325 at 1506.430 kb on + strand, within RALBFv3_RS21325 at 1506.430 kb on + strand, within RALBFv3_RS21325 at 1506.430 kb on + strand, within RALBFv3_RS21325 at 1506.431 kb on - strand, within RALBFv3_RS21325 at 1506.431 kb on - strand, within RALBFv3_RS21325 at 1506.432 kb on + strand, within RALBFv3_RS21325 at 1506.433 kb on - strand, within RALBFv3_RS21325 at 1506.433 kb on - strand, within RALBFv3_RS21325 at 1506.472 kb on - strand, within RALBFv3_RS21325 at 1506.483 kb on + strand, within RALBFv3_RS21325 at 1506.483 kb on + strand, within RALBFv3_RS21325 at 1506.578 kb on - strand at 1506.726 kb on + strand at 1506.726 kb on + strand at 1506.726 kb on + strand at 1506.726 kb on + strand at 1506.726 kb on + strand at 1506.726 kb on + strand at 1506.727 kb on - strand at 1506.727 kb on - strand at 1506.727 kb on - strand at 1507.111 kb on - strand, within RALBFv3_RS21330 at 1507.111 kb on - strand, within RALBFv3_RS21330 at 1507.243 kb on + strand at 1507.243 kb on + strand at 1507.244 kb on - strand at 1507.244 kb on - strand at 1507.244 kb on - strand at 1507.244 kb on - strand at 1507.253 kb on - strand at 1507.593 kb on + strand at 1507.593 kb on + strand at 1507.594 kb on - strand at 1507.594 kb on - strand at 1507.594 kb on - strand at 1507.659 kb on + strand, within RALBFv3_RS21335
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix remove 1,504,630 + -1.7 1,505,017 - RALBFv3_RS21320 0.36 -0.1 1,505,017 - RALBFv3_RS21320 0.36 +0.8 1,505,017 - RALBFv3_RS21320 0.36 -0.1 1,505,017 - RALBFv3_RS21320 0.36 +0.2 1,505,017 - RALBFv3_RS21320 0.36 +0.3 1,505,017 - RALBFv3_RS21320 0.36 +0.0 1,505,029 + RALBFv3_RS21320 0.37 -2.0 1,505,030 - RALBFv3_RS21320 0.37 +0.8 1,505,030 - RALBFv3_RS21320 0.37 +0.0 1,505,030 - RALBFv3_RS21320 0.37 +1.5 1,505,140 + RALBFv3_RS21320 0.49 +0.8 1,505,141 - RALBFv3_RS21320 0.49 +0.0 1,505,218 + RALBFv3_RS21320 0.57 +0.8 1,505,303 - RALBFv3_RS21320 0.66 -0.2 1,505,303 - RALBFv3_RS21320 0.66 +0.0 1,505,311 + RALBFv3_RS21320 0.66 +1.8 1,505,312 - RALBFv3_RS21320 0.66 -0.6 1,505,313 + RALBFv3_RS21320 0.67 +0.3 1,505,314 - RALBFv3_RS21320 0.67 -0.4 1,505,458 + RALBFv3_RS21320 0.82 -0.5 1,505,459 - RALBFv3_RS21320 0.82 +0.8 1,505,763 + RALBFv3_RS21325 0.13 -1.5 1,505,763 + RALBFv3_RS21325 0.13 -0.7 1,505,763 + RALBFv3_RS21325 0.13 -0.4 1,505,764 - RALBFv3_RS21325 0.13 -0.1 1,505,764 - RALBFv3_RS21325 0.13 -0.7 1,505,765 - RALBFv3_RS21325 0.13 -0.3 1,505,778 + RALBFv3_RS21325 0.14 -0.0 1,505,778 + RALBFv3_RS21325 0.14 +0.5 1,505,779 - RALBFv3_RS21325 0.14 -0.5 1,505,779 - RALBFv3_RS21325 0.14 -0.5 1,506,060 + RALBFv3_RS21325 0.41 -1.0 1,506,061 - RALBFv3_RS21325 0.41 -3.1 1,506,061 - RALBFv3_RS21325 0.41 -0.3 1,506,164 - RALBFv3_RS21325 0.51 -0.0 1,506,430 + RALBFv3_RS21325 0.76 -1.0 1,506,430 + RALBFv3_RS21325 0.76 +0.8 1,506,430 + RALBFv3_RS21325 0.76 -0.2 1,506,431 - RALBFv3_RS21325 0.76 +0.2 1,506,431 - RALBFv3_RS21325 0.76 -0.1 1,506,432 + RALBFv3_RS21325 0.76 -0.9 1,506,433 - RALBFv3_RS21325 0.76 -0.3 1,506,433 - RALBFv3_RS21325 0.76 -0.2 1,506,472 - RALBFv3_RS21325 0.80 +0.0 1,506,483 + RALBFv3_RS21325 0.81 -0.3 1,506,483 + RALBFv3_RS21325 0.81 +0.8 1,506,578 - -0.0 1,506,726 + +0.2 1,506,726 + -0.3 1,506,726 + -0.4 1,506,726 + -0.4 1,506,726 + -0.1 1,506,726 + -0.5 1,506,727 - -0.2 1,506,727 - +0.8 1,506,727 - -1.0 1,507,111 - RALBFv3_RS21330 0.67 -0.2 1,507,111 - RALBFv3_RS21330 0.67 -1.2 1,507,243 + -1.0 1,507,243 + -0.0 1,507,244 - -0.1 1,507,244 - -1.0 1,507,244 - +0.7 1,507,244 - -0.1 1,507,253 - -0.0 1,507,593 + +0.9 1,507,593 + -0.8 1,507,594 - -0.8 1,507,594 - -0.2 1,507,594 - +0.2 1,507,659 + RALBFv3_RS21335 0.18 -0.2
Or see this region's nucleotide sequence