Experiment: SMSA with Ralstonia_antibiotic_mix
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS18125 and RALBFv3_RS18130 are separated by 263 nucleotides RALBFv3_RS18130 and RALBFv3_RS18135 overlap by 23 nucleotides RALBFv3_RS18135 and RALBFv3_RS18140 are separated by 120 nucleotides RALBFv3_RS18140 and RALBFv3_RS18145 are separated by 54 nucleotides
RALBFv3_RS18125: RALBFv3_RS18125 - multidrug transporter subunit MdtA, at 593,228 to 594,565
_RS18125
RALBFv3_RS18130: RALBFv3_RS18130 - DNA-binding response regulator, at 594,829 to 595,566
_RS18130
RALBFv3_RS18135: RALBFv3_RS18135 - two-component sensor histidine kinase, at 595,544 to 596,833
_RS18135
RALBFv3_RS18140: RALBFv3_RS18140 - DUF333 domain-containing protein, at 596,954 to 597,409
_RS18140
RALBFv3_RS18145: RALBFv3_RS18145 - phospholipid carrier-dependent glycosyltransferase, at 597,464 to 599,119
_RS18145
Position (kb) 595
596
597 Strain fitness (log2 ratio) -7
-6
-5
-4
-3
-2
-1
0
1 at 594.664 kb on + strand at 594.664 kb on + strand at 594.664 kb on + strand at 594.664 kb on + strand at 594.707 kb on + strand at 594.708 kb on - strand at 594.710 kb on + strand at 594.710 kb on + strand at 594.711 kb on - strand at 594.720 kb on + strand at 594.721 kb on - strand at 594.754 kb on + strand at 594.754 kb on + strand at 594.755 kb on - strand at 594.755 kb on - strand at 594.810 kb on - strand at 595.129 kb on + strand, within RALBFv3_RS18130 at 595.130 kb on - strand, within RALBFv3_RS18130 at 595.391 kb on - strand, within RALBFv3_RS18130 at 595.483 kb on + strand, within RALBFv3_RS18130 at 595.510 kb on + strand at 596.129 kb on + strand, within RALBFv3_RS18135 at 596.352 kb on - strand, within RALBFv3_RS18135 at 596.851 kb on + strand at 596.857 kb on - strand at 596.863 kb on - strand at 597.400 kb on + strand at 597.400 kb on + strand at 597.400 kb on + strand at 597.401 kb on - strand at 597.401 kb on - strand at 597.696 kb on + strand, within RALBFv3_RS18145 at 597.696 kb on + strand, within RALBFv3_RS18145 at 597.696 kb on + strand, within RALBFv3_RS18145 at 597.697 kb on - strand, within RALBFv3_RS18145 at 597.699 kb on + strand, within RALBFv3_RS18145 at 597.699 kb on + strand, within RALBFv3_RS18145 at 597.699 kb on + strand, within RALBFv3_RS18145 at 597.700 kb on - strand, within RALBFv3_RS18145 at 597.700 kb on - strand, within RALBFv3_RS18145 at 597.732 kb on + strand, within RALBFv3_RS18145
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix remove 594,664 + -0.3 594,664 + -0.1 594,664 + -0.2 594,664 + +0.3 594,707 + -0.2 594,708 - -0.0 594,710 + +0.1 594,710 + -0.2 594,711 - -0.4 594,720 + -0.1 594,721 - +0.4 594,754 + +0.1 594,754 + -0.2 594,755 - -0.6 594,755 - -0.6 594,810 - -0.1 595,129 + RALBFv3_RS18130 0.41 -1.2 595,130 - RALBFv3_RS18130 0.41 +0.2 595,391 - RALBFv3_RS18130 0.76 +0.2 595,483 + RALBFv3_RS18130 0.89 -0.2 595,510 + -0.4 596,129 + RALBFv3_RS18135 0.45 +0.8 596,352 - RALBFv3_RS18135 0.63 -0.5 596,851 + -0.2 596,857 - -0.2 596,863 - -0.8 597,400 + +0.8 597,400 + +0.1 597,400 + +0.1 597,401 - -0.1 597,401 - -0.4 597,696 + RALBFv3_RS18145 0.14 -5.2 597,696 + RALBFv3_RS18145 0.14 -7.0 597,696 + RALBFv3_RS18145 0.14 -6.6 597,697 - RALBFv3_RS18145 0.14 -5.9 597,699 + RALBFv3_RS18145 0.14 -4.3 597,699 + RALBFv3_RS18145 0.14 -6.6 597,699 + RALBFv3_RS18145 0.14 -1.8 597,700 - RALBFv3_RS18145 0.14 +0.8 597,700 - RALBFv3_RS18145 0.14 -6.2 597,732 + RALBFv3_RS18145 0.16 -3.2
Or see this region's nucleotide sequence