Experiment: SMSA with Ralstonia_antibiotic_mix
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS17390 and RALBFv3_RS17395 are separated by 81 nucleotides RALBFv3_RS17395 and RALBFv3_RS17400 are separated by 164 nucleotides
RALBFv3_RS17390: RALBFv3_RS17390 - chemotaxis protein CheA, at 392,674 to 394,848
_RS17390
RALBFv3_RS17395: RALBFv3_RS17395 - chemotaxis protein CheW, at 394,930 to 395,421
_RS17395
RALBFv3_RS17400: RALBFv3_RS17400 - membrane protein, at 395,586 to 397,403
_RS17400
Position (kb) 394
395
396 Strain fitness (log2 ratio) -2
-1
0
1 at 394.267 kb on + strand, within RALBFv3_RS17390 at 394.268 kb on - strand, within RALBFv3_RS17390 at 394.477 kb on + strand, within RALBFv3_RS17390 at 394.477 kb on + strand, within RALBFv3_RS17390 at 394.477 kb on + strand, within RALBFv3_RS17390 at 394.477 kb on + strand, within RALBFv3_RS17390 at 394.478 kb on - strand, within RALBFv3_RS17390 at 394.498 kb on + strand, within RALBFv3_RS17390 at 394.499 kb on - strand, within RALBFv3_RS17390 at 394.645 kb on + strand at 394.645 kb on + strand at 394.646 kb on - strand at 394.646 kb on - strand at 394.646 kb on - strand at 394.887 kb on + strand at 394.887 kb on + strand at 394.888 kb on - strand at 394.888 kb on - strand at 394.888 kb on - strand at 394.888 kb on - strand at 394.888 kb on - strand at 394.891 kb on + strand at 394.892 kb on - strand at 394.900 kb on + strand at 394.900 kb on + strand at 394.900 kb on + strand at 394.901 kb on - strand at 394.978 kb on + strand at 394.978 kb on + strand at 394.978 kb on + strand at 394.978 kb on + strand at 394.978 kb on + strand at 394.979 kb on - strand at 394.979 kb on - strand at 395.009 kb on - strand, within RALBFv3_RS17395 at 395.168 kb on - strand, within RALBFv3_RS17395 at 395.176 kb on + strand, within RALBFv3_RS17395 at 395.176 kb on + strand, within RALBFv3_RS17395 at 395.176 kb on + strand, within RALBFv3_RS17395 at 395.177 kb on - strand, within RALBFv3_RS17395 at 395.311 kb on + strand, within RALBFv3_RS17395 at 395.312 kb on - strand, within RALBFv3_RS17395 at 395.312 kb on - strand, within RALBFv3_RS17395 at 395.312 kb on - strand, within RALBFv3_RS17395 at 395.312 kb on - strand, within RALBFv3_RS17395 at 395.312 kb on - strand, within RALBFv3_RS17395 at 395.476 kb on + strand at 395.477 kb on - strand at 395.477 kb on - strand at 395.477 kb on - strand at 395.554 kb on + strand at 395.556 kb on + strand at 395.556 kb on + strand at 395.556 kb on + strand at 395.556 kb on + strand at 395.556 kb on + strand at 395.556 kb on + strand at 395.556 kb on + strand at 395.556 kb on + strand at 395.557 kb on - strand at 395.557 kb on - strand at 395.557 kb on - strand at 395.558 kb on - strand at 395.598 kb on - strand at 395.696 kb on + strand at 395.715 kb on + strand at 395.715 kb on + strand at 395.715 kb on + strand at 395.715 kb on + strand at 395.716 kb on - strand at 395.716 kb on - strand at 395.716 kb on - strand at 396.051 kb on + strand, within RALBFv3_RS17400 at 396.118 kb on - strand, within RALBFv3_RS17400 at 396.118 kb on - strand, within RALBFv3_RS17400 at 396.154 kb on + strand, within RALBFv3_RS17400 at 396.155 kb on - strand, within RALBFv3_RS17400
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix remove 394,267 + RALBFv3_RS17390 0.73 -0.1 394,268 - RALBFv3_RS17390 0.73 +0.9 394,477 + RALBFv3_RS17390 0.83 -0.2 394,477 + RALBFv3_RS17390 0.83 -0.3 394,477 + RALBFv3_RS17390 0.83 +0.7 394,477 + RALBFv3_RS17390 0.83 -0.6 394,478 - RALBFv3_RS17390 0.83 -0.6 394,498 + RALBFv3_RS17390 0.84 -0.7 394,499 - RALBFv3_RS17390 0.84 -0.1 394,645 + +0.9 394,645 + +1.3 394,646 - +0.2 394,646 - -0.7 394,646 - -1.9 394,887 + +0.3 394,887 + -0.1 394,888 - -0.9 394,888 - -0.3 394,888 - -0.9 394,888 - -1.1 394,888 - -0.3 394,891 + -0.6 394,892 - -0.1 394,900 + +0.9 394,900 + -0.4 394,900 + -0.0 394,901 - -0.2 394,978 + +0.5 394,978 + +0.9 394,978 + +0.1 394,978 + -0.4 394,978 + -0.5 394,979 - -0.1 394,979 - -0.4 395,009 - RALBFv3_RS17395 0.16 -0.5 395,168 - RALBFv3_RS17395 0.48 -0.1 395,176 + RALBFv3_RS17395 0.50 -0.6 395,176 + RALBFv3_RS17395 0.50 +0.1 395,176 + RALBFv3_RS17395 0.50 -0.8 395,177 - RALBFv3_RS17395 0.50 -0.2 395,311 + RALBFv3_RS17395 0.77 +0.1 395,312 - RALBFv3_RS17395 0.78 +0.1 395,312 - RALBFv3_RS17395 0.78 -0.3 395,312 - RALBFv3_RS17395 0.78 -0.6 395,312 - RALBFv3_RS17395 0.78 -0.3 395,312 - RALBFv3_RS17395 0.78 -1.4 395,476 + +0.4 395,477 - -0.1 395,477 - -1.7 395,477 - -1.1 395,554 + -0.5 395,556 + -0.2 395,556 + -0.5 395,556 + -0.3 395,556 + +0.4 395,556 + +0.1 395,556 + -0.6 395,556 + -1.7 395,556 + +0.1 395,557 - +0.4 395,557 - -1.5 395,557 - -0.1 395,558 - +1.1 395,598 - -1.1 395,696 + -0.5 395,715 + +1.3 395,715 + +0.2 395,715 + -0.1 395,715 + -0.5 395,716 - +0.9 395,716 - -1.6 395,716 - -1.0 396,051 + RALBFv3_RS17400 0.26 -0.2 396,118 - RALBFv3_RS17400 0.29 -0.4 396,118 - RALBFv3_RS17400 0.29 -0.2 396,154 + RALBFv3_RS17400 0.31 +0.0 396,155 - RALBFv3_RS17400 0.31 +0.5
Or see this region's nucleotide sequence