Experiment: SMSA with Ralstonia_antibiotic_mix
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS08475 and RALBFv3_RS08480 overlap by 4 nucleotides RALBFv3_RS08480 and RALBFv3_RS08485 are separated by 123 nucleotides
RALBFv3_RS08475: RALBFv3_RS08475 - flavocytochrome C sulfide dehydrogenase, at 1,869,224 to 1,870,516
_RS08475
RALBFv3_RS08480: RALBFv3_RS08480 - hypothetical protein, at 1,870,513 to 1,870,863
_RS08480
RALBFv3_RS08485: RALBFv3_RS08485 - BON domain-containing protein, at 1,870,987 to 1,871,805
_RS08485
Position (kb)
1870
1871 Strain fitness (log2 ratio)
-1
0
1 at 1869.750 kb on + strand, within RALBFv3_RS08475 at 1869.751 kb on - strand, within RALBFv3_RS08475 at 1869.751 kb on - strand, within RALBFv3_RS08475 at 1869.751 kb on - strand, within RALBFv3_RS08475 at 1869.751 kb on - strand, within RALBFv3_RS08475 at 1870.353 kb on + strand, within RALBFv3_RS08475 at 1870.353 kb on + strand, within RALBFv3_RS08475 at 1870.353 kb on + strand, within RALBFv3_RS08475 at 1870.354 kb on - strand, within RALBFv3_RS08475 at 1870.354 kb on - strand, within RALBFv3_RS08475 at 1870.894 kb on - strand at 1870.894 kb on - strand at 1870.899 kb on - strand at 1870.899 kb on - strand at 1871.213 kb on + strand, within RALBFv3_RS08485 at 1871.309 kb on + strand, within RALBFv3_RS08485 at 1871.399 kb on + strand, within RALBFv3_RS08485 at 1871.399 kb on + strand, within RALBFv3_RS08485 at 1871.399 kb on + strand, within RALBFv3_RS08485 at 1871.400 kb on - strand, within RALBFv3_RS08485 at 1871.400 kb on - strand, within RALBFv3_RS08485 at 1871.400 kb on - strand, within RALBFv3_RS08485 at 1871.400 kb on - strand, within RALBFv3_RS08485 at 1871.490 kb on - strand, within RALBFv3_RS08485 at 1871.555 kb on + strand, within RALBFv3_RS08485 at 1871.555 kb on + strand, within RALBFv3_RS08485 at 1871.556 kb on - strand, within RALBFv3_RS08485 at 1871.556 kb on - strand, within RALBFv3_RS08485 at 1871.556 kb on - strand, within RALBFv3_RS08485 at 1871.588 kb on + strand, within RALBFv3_RS08485 at 1871.588 kb on + strand, within RALBFv3_RS08485 at 1871.589 kb on - strand, within RALBFv3_RS08485 at 1871.589 kb on - strand, within RALBFv3_RS08485 at 1871.589 kb on - strand, within RALBFv3_RS08485 at 1871.589 kb on - strand, within RALBFv3_RS08485
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix remove 1,869,750 + RALBFv3_RS08475 0.41 +0.6 1,869,751 - RALBFv3_RS08475 0.41 +0.5 1,869,751 - RALBFv3_RS08475 0.41 -0.1 1,869,751 - RALBFv3_RS08475 0.41 -0.5 1,869,751 - RALBFv3_RS08475 0.41 -0.3 1,870,353 + RALBFv3_RS08475 0.87 -0.3 1,870,353 + RALBFv3_RS08475 0.87 -0.1 1,870,353 + RALBFv3_RS08475 0.87 +0.1 1,870,354 - RALBFv3_RS08475 0.87 +0.1 1,870,354 - RALBFv3_RS08475 0.87 +0.4 1,870,894 - +1.2 1,870,894 - -0.4 1,870,899 - -0.4 1,870,899 - +1.2 1,871,213 + RALBFv3_RS08485 0.28 +1.2 1,871,309 + RALBFv3_RS08485 0.39 +0.2 1,871,399 + RALBFv3_RS08485 0.50 +0.4 1,871,399 + RALBFv3_RS08485 0.50 -0.5 1,871,399 + RALBFv3_RS08485 0.50 -0.5 1,871,400 - RALBFv3_RS08485 0.50 +1.5 1,871,400 - RALBFv3_RS08485 0.50 -0.6 1,871,400 - RALBFv3_RS08485 0.50 -0.3 1,871,400 - RALBFv3_RS08485 0.50 -0.0 1,871,490 - RALBFv3_RS08485 0.61 +1.2 1,871,555 + RALBFv3_RS08485 0.69 -0.7 1,871,555 + RALBFv3_RS08485 0.69 -0.3 1,871,556 - RALBFv3_RS08485 0.69 +0.9 1,871,556 - RALBFv3_RS08485 0.69 +0.2 1,871,556 - RALBFv3_RS08485 0.69 +1.0 1,871,588 + RALBFv3_RS08485 0.73 +0.4 1,871,588 + RALBFv3_RS08485 0.73 -0.8 1,871,589 - RALBFv3_RS08485 0.74 -0.4 1,871,589 - RALBFv3_RS08485 0.74 +0.2 1,871,589 - RALBFv3_RS08485 0.74 +1.2 1,871,589 - RALBFv3_RS08485 0.74 +0.1
Or see this region's nucleotide sequence