Strain Fitness in Ralstonia solanacearum IBSBF1503 around RALBFv3_RS04495

Experiment: SMSA with Ralstonia_antibiotic_mix

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRALBFv3_RS04490 and RALBFv3_RS04495 overlap by 4 nucleotidesRALBFv3_RS04495 and RALBFv3_RS04500 are separated by 531 nucleotidesRALBFv3_RS04500 and RALBFv3_RS04505 overlap by 11 nucleotides RALBFv3_RS04490: RALBFv3_RS04490 - iron ABC transporter permease, at 1,008,377 to 1,009,384 _RS04490 RALBFv3_RS04495: RALBFv3_RS04495 - TonB-dependent receptor, at 1,009,381 to 1,011,261 _RS04495 RALBFv3_RS04500: RALBFv3_RS04500 - hypothetical protein, at 1,011,793 to 1,012,113 _RS04500 RALBFv3_RS04505: RALBFv3_RS04505 - cell division protein ZapA, at 1,012,103 to 1,012,420 _RS04505 Position (kb) 1009 1010 1011 1012Strain fitness (log2 ratio) -1 0 1 2at 1008.651 kb on + strand, within RALBFv3_RS04490at 1008.651 kb on + strand, within RALBFv3_RS04490at 1008.652 kb on - strand, within RALBFv3_RS04490at 1008.874 kb on + strand, within RALBFv3_RS04490at 1009.102 kb on - strand, within RALBFv3_RS04490at 1009.102 kb on - strand, within RALBFv3_RS04490at 1009.102 kb on - strand, within RALBFv3_RS04490at 1009.102 kb on - strand, within RALBFv3_RS04490at 1009.446 kb on - strandat 1009.464 kb on - strandat 1009.464 kb on - strandat 1009.713 kb on + strand, within RALBFv3_RS04495at 1009.739 kb on + strand, within RALBFv3_RS04495at 1009.740 kb on - strand, within RALBFv3_RS04495at 1009.851 kb on - strand, within RALBFv3_RS04495at 1009.899 kb on - strand, within RALBFv3_RS04495at 1009.965 kb on - strand, within RALBFv3_RS04495at 1009.967 kb on + strand, within RALBFv3_RS04495at 1009.968 kb on - strand, within RALBFv3_RS04495at 1009.968 kb on - strand, within RALBFv3_RS04495at 1009.968 kb on - strand, within RALBFv3_RS04495at 1010.057 kb on + strand, within RALBFv3_RS04495at 1010.061 kb on - strand, within RALBFv3_RS04495at 1010.183 kb on + strand, within RALBFv3_RS04495at 1010.183 kb on + strand, within RALBFv3_RS04495at 1010.311 kb on + strand, within RALBFv3_RS04495at 1010.312 kb on - strand, within RALBFv3_RS04495at 1010.312 kb on - strand, within RALBFv3_RS04495at 1010.402 kb on + strand, within RALBFv3_RS04495at 1010.403 kb on - strand, within RALBFv3_RS04495at 1010.435 kb on + strand, within RALBFv3_RS04495at 1010.435 kb on + strand, within RALBFv3_RS04495at 1010.435 kb on + strand, within RALBFv3_RS04495at 1010.436 kb on - strand, within RALBFv3_RS04495at 1010.436 kb on - strand, within RALBFv3_RS04495at 1010.471 kb on + strand, within RALBFv3_RS04495at 1010.472 kb on - strand, within RALBFv3_RS04495at 1010.567 kb on + strand, within RALBFv3_RS04495at 1011.090 kb on + strandat 1011.184 kb on - strandat 1011.279 kb on + strandat 1011.280 kb on - strandat 1011.464 kb on + strandat 1011.464 kb on + strandat 1011.465 kb on - strandat 1011.728 kb on - strandat 1012.111 kb on + strandat 1012.145 kb on + strand, within RALBFv3_RS04505

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix
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1,008,651 + RALBFv3_RS04490 0.27 +0.4
1,008,651 + RALBFv3_RS04490 0.27 -0.7
1,008,652 - RALBFv3_RS04490 0.27 +1.1
1,008,874 + RALBFv3_RS04490 0.49 +0.1
1,009,102 - RALBFv3_RS04490 0.72 -0.3
1,009,102 - RALBFv3_RS04490 0.72 +0.4
1,009,102 - RALBFv3_RS04490 0.72 -0.9
1,009,102 - RALBFv3_RS04490 0.72 +0.7
1,009,446 - -0.0
1,009,464 - -0.1
1,009,464 - +0.6
1,009,713 + RALBFv3_RS04495 0.18 -0.3
1,009,739 + RALBFv3_RS04495 0.19 -0.7
1,009,740 - RALBFv3_RS04495 0.19 -1.1
1,009,851 - RALBFv3_RS04495 0.25 +0.6
1,009,899 - RALBFv3_RS04495 0.28 -0.2
1,009,965 - RALBFv3_RS04495 0.31 -1.2
1,009,967 + RALBFv3_RS04495 0.31 +0.0
1,009,968 - RALBFv3_RS04495 0.31 -1.2
1,009,968 - RALBFv3_RS04495 0.31 -1.2
1,009,968 - RALBFv3_RS04495 0.31 -1.0
1,010,057 + RALBFv3_RS04495 0.36 +0.5
1,010,061 - RALBFv3_RS04495 0.36 +2.1
1,010,183 + RALBFv3_RS04495 0.43 -0.0
1,010,183 + RALBFv3_RS04495 0.43 -0.7
1,010,311 + RALBFv3_RS04495 0.49 +0.1
1,010,312 - RALBFv3_RS04495 0.49 +1.1
1,010,312 - RALBFv3_RS04495 0.49 +1.1
1,010,402 + RALBFv3_RS04495 0.54 -0.1
1,010,403 - RALBFv3_RS04495 0.54 -1.4
1,010,435 + RALBFv3_RS04495 0.56 +0.1
1,010,435 + RALBFv3_RS04495 0.56 -0.3
1,010,435 + RALBFv3_RS04495 0.56 +0.6
1,010,436 - RALBFv3_RS04495 0.56 -0.3
1,010,436 - RALBFv3_RS04495 0.56 -0.3
1,010,471 + RALBFv3_RS04495 0.58 +1.1
1,010,472 - RALBFv3_RS04495 0.58 -0.4
1,010,567 + RALBFv3_RS04495 0.63 -0.9
1,011,090 + -0.9
1,011,184 - -0.7
1,011,279 + -0.4
1,011,280 - +0.6
1,011,464 + -1.2
1,011,464 + -0.3
1,011,465 - -0.7
1,011,728 - -0.8
1,012,111 + +0.0
1,012,145 + RALBFv3_RS04505 0.13 +1.5

Or see this region's nucleotide sequence