Experiment: SMSA no antibiotic control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS05185 and RALBFv3_RS05190 are separated by 362 nucleotides RALBFv3_RS05190 and RALBFv3_RS05195 are separated by 139 nucleotides RALBFv3_RS05195 and RALBFv3_RS05200 are separated by 259 nucleotides
RALBFv3_RS05185: RALBFv3_RS05185 - ornithine carbamoyltransferase, at 1,149,505 to 1,150,431
_RS05185
RALBFv3_RS05190: RALBFv3_RS05190 - DUF3579 domain-containing protein, at 1,150,794 to 1,151,117
_RS05190
RALBFv3_RS05195: RALBFv3_RS05195 - 30S ribosomal protein S20, at 1,151,257 to 1,151,523
_RS05195
RALBFv3_RS05200: RALBFv3_RS05200 - murein biosynthesis integral membrane protein MurJ, at 1,151,783 to 1,153,375
_RS05200
Position (kb)
1151
1152 Strain fitness (log2 ratio)
-2
-1
0
1 at 1150.432 kb on - strand at 1150.470 kb on - strand at 1150.610 kb on - strand at 1150.612 kb on - strand at 1150.613 kb on + strand at 1150.613 kb on + strand at 1150.620 kb on + strand at 1150.623 kb on + strand at 1150.939 kb on - strand, within RALBFv3_RS05190 at 1150.947 kb on + strand, within RALBFv3_RS05190 at 1151.714 kb on - strand at 1151.714 kb on - strand at 1151.715 kb on + strand at 1151.715 kb on + strand at 1151.715 kb on + strand at 1151.718 kb on + strand at 1151.719 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA no antibiotic control remove 1,150,432 - -0.4 1,150,470 - +0.1 1,150,610 - +0.1 1,150,612 - +0.7 1,150,613 + -0.3 1,150,613 + +1.2 1,150,620 + -0.2 1,150,623 + +1.2 1,150,939 - RALBFv3_RS05190 0.45 -0.8 1,150,947 + RALBFv3_RS05190 0.47 -1.2 1,151,714 - +0.2 1,151,714 - +0.8 1,151,715 + +0.8 1,151,715 + -2.5 1,151,715 + -0.4 1,151,718 + -1.6 1,151,719 - +0.0
Or see this region's nucleotide sequence