Strain Fitness in Ralstonia solanacearum IBSBF1503 around RALBFv3_RS05195

Experiment: SMSA no antibiotic control

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRALBFv3_RS05185 and RALBFv3_RS05190 are separated by 362 nucleotidesRALBFv3_RS05190 and RALBFv3_RS05195 are separated by 139 nucleotidesRALBFv3_RS05195 and RALBFv3_RS05200 are separated by 259 nucleotides RALBFv3_RS05185: RALBFv3_RS05185 - ornithine carbamoyltransferase, at 1,149,505 to 1,150,431 _RS05185 RALBFv3_RS05190: RALBFv3_RS05190 - DUF3579 domain-containing protein, at 1,150,794 to 1,151,117 _RS05190 RALBFv3_RS05195: RALBFv3_RS05195 - 30S ribosomal protein S20, at 1,151,257 to 1,151,523 _RS05195 RALBFv3_RS05200: RALBFv3_RS05200 - murein biosynthesis integral membrane protein MurJ, at 1,151,783 to 1,153,375 _RS05200 Position (kb) 1151 1152Strain fitness (log2 ratio) -2 -1 0 1at 1150.432 kb on - strandat 1150.470 kb on - strandat 1150.610 kb on - strandat 1150.612 kb on - strandat 1150.613 kb on + strandat 1150.613 kb on + strandat 1150.620 kb on + strandat 1150.623 kb on + strandat 1150.939 kb on - strand, within RALBFv3_RS05190at 1150.947 kb on + strand, within RALBFv3_RS05190at 1151.714 kb on - strandat 1151.714 kb on - strandat 1151.715 kb on + strandat 1151.715 kb on + strandat 1151.715 kb on + strandat 1151.718 kb on + strandat 1151.719 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA no antibiotic control
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1,150,432 - -0.4
1,150,470 - +0.1
1,150,610 - +0.1
1,150,612 - +0.7
1,150,613 + -0.3
1,150,613 + +1.2
1,150,620 + -0.2
1,150,623 + +1.2
1,150,939 - RALBFv3_RS05190 0.45 -0.8
1,150,947 + RALBFv3_RS05190 0.47 -1.2
1,151,714 - +0.2
1,151,714 - +0.8
1,151,715 + +0.8
1,151,715 + -2.5
1,151,715 + -0.4
1,151,718 + -1.6
1,151,719 - +0.0

Or see this region's nucleotide sequence