Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol_and_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS23420 and RALBFv3_RS23425 are separated by 13 nucleotides RALBFv3_RS23425 and RALBFv3_RS23430 overlap by 4 nucleotides RALBFv3_RS23430 and RALBFv3_RS23435 are separated by 209 nucleotides RALBFv3_RS23435 and RALBFv3_RS23440 overlap by 4 nucleotides
RALBFv3_RS23420: RALBFv3_RS23420 - PepSY domain-containing protein, at 2,035,135 to 2,036,757
_RS23420
RALBFv3_RS23425: RALBFv3_RS23425 - hypothetical protein, at 2,036,771 to 2,037,037
_RS23425
RALBFv3_RS23430: RALBFv3_RS23430 - hypothetical protein, at 2,037,034 to 2,037,345
_RS23430
RALBFv3_RS23435: RALBFv3_RS23435 - hypothetical protein, at 2,037,555 to 2,037,893
_RS23435
RALBFv3_RS23440: RALBFv3_RS23440 - CusA/CzcA family heavy metal efflux RND transporter, at 2,037,890 to 2,041,054
_RS23440
Position (kb)
2037
2038 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2036.273 kb on - strand, within RALBFv3_RS23420 at 2036.288 kb on + strand, within RALBFv3_RS23420 at 2036.289 kb on - strand, within RALBFv3_RS23420 at 2036.315 kb on + strand, within RALBFv3_RS23420 at 2036.315 kb on + strand, within RALBFv3_RS23420 at 2036.315 kb on + strand, within RALBFv3_RS23420 at 2036.315 kb on + strand, within RALBFv3_RS23420 at 2036.315 kb on + strand, within RALBFv3_RS23420 at 2036.315 kb on + strand, within RALBFv3_RS23420 at 2036.315 kb on + strand, within RALBFv3_RS23420 at 2036.315 kb on + strand, within RALBFv3_RS23420 at 2036.315 kb on + strand, within RALBFv3_RS23420 at 2036.315 kb on + strand, within RALBFv3_RS23420 at 2036.316 kb on - strand, within RALBFv3_RS23420 at 2036.345 kb on + strand, within RALBFv3_RS23420 at 2036.475 kb on - strand, within RALBFv3_RS23420 at 2036.522 kb on + strand, within RALBFv3_RS23420 at 2036.522 kb on + strand, within RALBFv3_RS23420 at 2036.523 kb on - strand, within RALBFv3_RS23420 at 2036.718 kb on + strand at 2036.718 kb on + strand at 2036.718 kb on + strand at 2036.719 kb on - strand at 2036.719 kb on - strand at 2036.719 kb on - strand at 2036.719 kb on - strand at 2036.732 kb on + strand at 2036.733 kb on - strand at 2036.740 kb on + strand at 2036.740 kb on + strand at 2036.740 kb on + strand at 2036.740 kb on + strand at 2036.740 kb on + strand at 2036.740 kb on + strand at 2036.740 kb on + strand at 2036.740 kb on + strand at 2036.740 kb on + strand at 2036.740 kb on + strand at 2036.741 kb on - strand at 2036.741 kb on - strand at 2036.741 kb on - strand at 2037.249 kb on + strand, within RALBFv3_RS23430 at 2037.297 kb on - strand, within RALBFv3_RS23430 at 2037.429 kb on + strand at 2037.430 kb on - strand at 2037.430 kb on - strand at 2037.430 kb on - strand at 2037.886 kb on + strand at 2037.887 kb on - strand at 2037.887 kb on - strand at 2037.887 kb on - strand at 2037.887 kb on - strand at 2037.924 kb on + strand at 2037.924 kb on + strand at 2037.924 kb on + strand at 2037.925 kb on - strand at 2037.960 kb on + strand at 2038.216 kb on - strand, within RALBFv3_RS23440 at 2038.296 kb on + strand, within RALBFv3_RS23440 at 2038.296 kb on + strand, within RALBFv3_RS23440 at 2038.296 kb on + strand, within RALBFv3_RS23440 at 2038.296 kb on + strand, within RALBFv3_RS23440 at 2038.296 kb on + strand, within RALBFv3_RS23440 at 2038.297 kb on - strand, within RALBFv3_RS23440
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol_and_CrystalViolet remove 2,036,273 - RALBFv3_RS23420 0.70 +0.7 2,036,288 + RALBFv3_RS23420 0.71 -0.7 2,036,289 - RALBFv3_RS23420 0.71 -0.8 2,036,315 + RALBFv3_RS23420 0.73 -1.2 2,036,315 + RALBFv3_RS23420 0.73 +2.4 2,036,315 + RALBFv3_RS23420 0.73 +0.1 2,036,315 + RALBFv3_RS23420 0.73 +0.4 2,036,315 + RALBFv3_RS23420 0.73 -0.2 2,036,315 + RALBFv3_RS23420 0.73 -0.2 2,036,315 + RALBFv3_RS23420 0.73 -0.6 2,036,315 + RALBFv3_RS23420 0.73 +0.3 2,036,315 + RALBFv3_RS23420 0.73 +0.1 2,036,315 + RALBFv3_RS23420 0.73 +0.5 2,036,316 - RALBFv3_RS23420 0.73 -0.2 2,036,345 + RALBFv3_RS23420 0.75 -0.6 2,036,475 - RALBFv3_RS23420 0.83 +0.2 2,036,522 + RALBFv3_RS23420 0.85 -0.9 2,036,522 + RALBFv3_RS23420 0.85 -0.0 2,036,523 - RALBFv3_RS23420 0.86 -0.7 2,036,718 + -0.7 2,036,718 + +1.4 2,036,718 + -0.3 2,036,719 - -0.0 2,036,719 - +0.1 2,036,719 - +1.4 2,036,719 - -0.3 2,036,732 + -0.6 2,036,733 - +0.7 2,036,740 + -0.4 2,036,740 + -0.5 2,036,740 + -0.2 2,036,740 + -0.1 2,036,740 + -0.5 2,036,740 + +0.5 2,036,740 + +0.4 2,036,740 + +0.1 2,036,740 + -0.4 2,036,740 + -0.2 2,036,741 - +1.4 2,036,741 - -0.3 2,036,741 - -0.2 2,037,249 + RALBFv3_RS23430 0.69 +0.3 2,037,297 - RALBFv3_RS23430 0.84 -0.5 2,037,429 + +1.4 2,037,430 - +0.1 2,037,430 - +0.1 2,037,430 - +0.1 2,037,886 + +0.0 2,037,887 - -0.4 2,037,887 - -1.3 2,037,887 - -0.6 2,037,887 - -0.3 2,037,924 + +2.4 2,037,924 + -0.4 2,037,924 + +0.3 2,037,925 - +0.8 2,037,960 + -0.3 2,038,216 - RALBFv3_RS23440 0.10 -2.3 2,038,296 + RALBFv3_RS23440 0.13 -0.2 2,038,296 + RALBFv3_RS23440 0.13 -0.1 2,038,296 + RALBFv3_RS23440 0.13 +0.2 2,038,296 + RALBFv3_RS23440 0.13 -0.4 2,038,296 + RALBFv3_RS23440 0.13 +0.0 2,038,297 - RALBFv3_RS23440 0.13 +0.8
Or see this region's nucleotide sequence