Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol_and_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS03585 and RALBFv3_RS03590 are separated by 25 nucleotides RALBFv3_RS03590 and RALBFv3_RS03595 are separated by 52 nucleotides RALBFv3_RS03595 and RALBFv3_RS03600 are separated by 48 nucleotides
RALBFv3_RS03585: RALBFv3_RS03585 - hypothetical protein, at 785,340 to 786,812
_RS03585
RALBFv3_RS03590: RALBFv3_RS03590 - alkylhydroperoxidase, at 786,838 to 787,443
_RS03590
RALBFv3_RS03595: RALBFv3_RS03595 - lipid A export permease/ATP-binding protein MsbA, at 787,496 to 789,274
_RS03595
RALBFv3_RS03600: RALBFv3_RS03600 - glycosyl transferase, at 789,323 to 790,183
_RS03600
Position (kb) 787
788
789
790 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 786.600 kb on + strand, within RALBFv3_RS03585 at 786.981 kb on + strand, within RALBFv3_RS03590 at 787.130 kb on + strand, within RALBFv3_RS03590 at 787.130 kb on + strand, within RALBFv3_RS03590 at 787.131 kb on - strand, within RALBFv3_RS03590 at 787.131 kb on - strand, within RALBFv3_RS03590 at 787.498 kb on - strand at 787.498 kb on - strand at 787.533 kb on + strand at 787.534 kb on - strand at 789.456 kb on + strand, within RALBFv3_RS03600 at 789.457 kb on - strand, within RALBFv3_RS03600 at 789.457 kb on - strand, within RALBFv3_RS03600 at 789.457 kb on - strand, within RALBFv3_RS03600 at 789.555 kb on + strand, within RALBFv3_RS03600 at 789.661 kb on - strand, within RALBFv3_RS03600 at 789.810 kb on - strand, within RALBFv3_RS03600 at 789.810 kb on - strand, within RALBFv3_RS03600 at 789.943 kb on - strand, within RALBFv3_RS03600 at 789.951 kb on + strand, within RALBFv3_RS03600 at 789.951 kb on + strand, within RALBFv3_RS03600 at 789.951 kb on + strand, within RALBFv3_RS03600 at 789.952 kb on - strand, within RALBFv3_RS03600 at 790.021 kb on + strand, within RALBFv3_RS03600 at 790.022 kb on - strand, within RALBFv3_RS03600 at 790.170 kb on - strand at 790.183 kb on + strand at 790.183 kb on + strand at 790.183 kb on + strand at 790.184 kb on - strand at 790.195 kb on + strand at 790.196 kb on - strand at 790.249 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol_and_CrystalViolet remove 786,600 + RALBFv3_RS03585 0.86 -0.7 786,981 + RALBFv3_RS03590 0.24 -0.6 787,130 + RALBFv3_RS03590 0.48 +0.5 787,130 + RALBFv3_RS03590 0.48 +0.0 787,131 - RALBFv3_RS03590 0.48 +0.0 787,131 - RALBFv3_RS03590 0.48 -0.4 787,498 - +0.2 787,498 - +1.5 787,533 + +0.5 787,534 - -0.3 789,456 + RALBFv3_RS03600 0.15 +0.5 789,457 - RALBFv3_RS03600 0.16 -2.2 789,457 - RALBFv3_RS03600 0.16 +1.5 789,457 - RALBFv3_RS03600 0.16 -2.1 789,555 + RALBFv3_RS03600 0.27 +1.5 789,661 - RALBFv3_RS03600 0.39 -3.0 789,810 - RALBFv3_RS03600 0.57 -0.5 789,810 - RALBFv3_RS03600 0.57 -3.7 789,943 - RALBFv3_RS03600 0.72 -2.0 789,951 + RALBFv3_RS03600 0.73 -1.3 789,951 + RALBFv3_RS03600 0.73 -1.6 789,951 + RALBFv3_RS03600 0.73 -1.2 789,952 - RALBFv3_RS03600 0.73 -2.3 790,021 + RALBFv3_RS03600 0.81 -0.1 790,022 - RALBFv3_RS03600 0.81 -3.9 790,170 - -3.8 790,183 + -2.2 790,183 + +0.5 790,183 + -0.5 790,184 - -1.0 790,195 + -0.5 790,196 - +0.5 790,249 + +1.5
Or see this region's nucleotide sequence