Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol_and_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS01300 and RALBFv3_RS01305 are separated by 12 nucleotides RALBFv3_RS01305 and RALBFv3_RS01310 are separated by 76 nucleotides RALBFv3_RS01310 and RALBFv3_RS01315 are separated by 79 nucleotides RALBFv3_RS01315 and RALBFv3_RS01320 are separated by 68 nucleotides
RALBFv3_RS01300: RALBFv3_RS01300 - glucose-6-phosphate isomerase, at 292,832 to 294,451
_RS01300
RALBFv3_RS01305: RALBFv3_RS01305 - ABC transporter ATP-binding protein, at 294,464 to 295,174
_RS01305
RALBFv3_RS01310: RALBFv3_RS01310 - arylesterase, at 295,251 to 295,868
_RS01310
RALBFv3_RS01315: RALBFv3_RS01315 - signal peptidase I, at 295,948 to 296,640
_RS01315
RALBFv3_RS01320: RALBFv3_RS01320 - peptidylprolyl isomerase, at 296,709 to 298,655
_RS01320
Position (kb) 295
296 Strain fitness (log2 ratio) -1
0
1 at 295.096 kb on + strand, within RALBFv3_RS01305 at 295.096 kb on + strand, within RALBFv3_RS01305 at 295.191 kb on - strand at 295.193 kb on + strand at 295.194 kb on - strand at 295.416 kb on + strand, within RALBFv3_RS01310 at 295.514 kb on - strand, within RALBFv3_RS01310 at 295.650 kb on + strand, within RALBFv3_RS01310 at 295.708 kb on - strand, within RALBFv3_RS01310 at 295.899 kb on + strand at 296.058 kb on - strand, within RALBFv3_RS01315 at 296.284 kb on + strand, within RALBFv3_RS01315 at 296.599 kb on + strand at 296.801 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol_and_CrystalViolet remove 295,096 + RALBFv3_RS01305 0.89 -0.0 295,096 + RALBFv3_RS01305 0.89 +0.9 295,191 - -0.2 295,193 + +0.4 295,194 - +1.4 295,416 + RALBFv3_RS01310 0.27 +0.5 295,514 - RALBFv3_RS01310 0.43 -0.2 295,650 + RALBFv3_RS01310 0.65 +0.1 295,708 - RALBFv3_RS01310 0.74 +1.4 295,899 + +0.4 296,058 - RALBFv3_RS01315 0.16 +0.5 296,284 + RALBFv3_RS01315 0.48 -0.1 296,599 + -1.2 296,801 - -1.2
Or see this region's nucleotide sequence