Strain Fitness in Ralstonia solanacearum IBSBF1503 around RALBFv3_RS10435

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol_and_CrystalViolet

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRALBFv3_RS10420 and RALBFv3_RS10425 overlap by 4 nucleotidesRALBFv3_RS10425 and RALBFv3_RS10430 are separated by 16 nucleotidesRALBFv3_RS10430 and RALBFv3_RS10435 are separated by 19 nucleotidesRALBFv3_RS10435 and RALBFv3_RS10440 are separated by 1 nucleotidesRALBFv3_RS10440 and RALBFv3_RS10445 overlap by 2 nucleotides RALBFv3_RS10420: RALBFv3_RS10420 - MBL fold metallo-hydrolase, at 2,320,613 to 2,321,575 _RS10420 RALBFv3_RS10425: RALBFv3_RS10425 - DUF1289 domain-containing protein, at 2,321,572 to 2,321,838 _RS10425 RALBFv3_RS10430: RALBFv3_RS10430 - YbaK/EbsC family protein, at 2,321,855 to 2,322,358 _RS10430 RALBFv3_RS10435: RALBFv3_RS10435 - hydroxymethylglutaryl-CoA lyase, at 2,322,378 to 2,323,307 _RS10435 RALBFv3_RS10440: RALBFv3_RS10440 - glyoxylate/hydroxypyruvate reductase A, at 2,323,309 to 2,324,250 _RS10440 RALBFv3_RS10445: RALBFv3_RS10445 - hypothetical protein, at 2,324,249 to 2,324,524 _RS10445 Position (kb) 2322 2323 2324Strain fitness (log2 ratio) -1 0 1 2at 2322.042 kb on + strand, within RALBFv3_RS10430at 2322.383 kb on + strandat 2322.384 kb on - strandat 2322.384 kb on - strandat 2322.538 kb on + strand, within RALBFv3_RS10435at 2322.539 kb on - strand, within RALBFv3_RS10435at 2322.539 kb on - strand, within RALBFv3_RS10435at 2322.673 kb on + strand, within RALBFv3_RS10435at 2322.673 kb on + strand, within RALBFv3_RS10435at 2322.674 kb on - strand, within RALBFv3_RS10435at 2322.823 kb on - strand, within RALBFv3_RS10435at 2322.859 kb on + strand, within RALBFv3_RS10435at 2323.078 kb on + strand, within RALBFv3_RS10435at 2323.079 kb on - strand, within RALBFv3_RS10435at 2323.586 kb on + strand, within RALBFv3_RS10440at 2323.587 kb on - strand, within RALBFv3_RS10440at 2323.587 kb on - strand, within RALBFv3_RS10440at 2323.797 kb on + strand, within RALBFv3_RS10440at 2323.798 kb on - strand, within RALBFv3_RS10440at 2323.899 kb on - strand, within RALBFv3_RS10440at 2323.944 kb on - strand, within RALBFv3_RS10440at 2323.944 kb on - strand, within RALBFv3_RS10440at 2324.129 kb on + strand, within RALBFv3_RS10440at 2324.129 kb on + strand, within RALBFv3_RS10440at 2324.234 kb on + strandat 2324.235 kb on - strandat 2324.235 kb on - strandat 2324.235 kb on - strandat 2324.290 kb on + strand, within RALBFv3_RS10445

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol_and_CrystalViolet
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2,322,042 + RALBFv3_RS10430 0.37 -0.6
2,322,383 + -1.5
2,322,384 - -1.2
2,322,384 - +0.0
2,322,538 + RALBFv3_RS10435 0.17 +0.4
2,322,539 - RALBFv3_RS10435 0.17 -0.6
2,322,539 - RALBFv3_RS10435 0.17 +0.1
2,322,673 + RALBFv3_RS10435 0.32 -1.1
2,322,673 + RALBFv3_RS10435 0.32 -1.4
2,322,674 - RALBFv3_RS10435 0.32 +2.4
2,322,823 - RALBFv3_RS10435 0.48 -1.2
2,322,859 + RALBFv3_RS10435 0.52 -0.4
2,323,078 + RALBFv3_RS10435 0.75 -1.0
2,323,079 - RALBFv3_RS10435 0.75 -1.5
2,323,586 + RALBFv3_RS10440 0.29 -0.2
2,323,587 - RALBFv3_RS10440 0.30 +0.5
2,323,587 - RALBFv3_RS10440 0.30 +1.0
2,323,797 + RALBFv3_RS10440 0.52 -0.4
2,323,798 - RALBFv3_RS10440 0.52 -0.5
2,323,899 - RALBFv3_RS10440 0.63 -0.3
2,323,944 - RALBFv3_RS10440 0.67 -0.1
2,323,944 - RALBFv3_RS10440 0.67 -0.2
2,324,129 + RALBFv3_RS10440 0.87 -0.3
2,324,129 + RALBFv3_RS10440 0.87 -0.1
2,324,234 + -0.4
2,324,235 - +0.1
2,324,235 - -0.3
2,324,235 - -0.8
2,324,290 + RALBFv3_RS10445 0.15 +0.4

Or see this region's nucleotide sequence