Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS03110 and RALBFv3_RS03115 are separated by 44 nucleotides RALBFv3_RS03115 and RALBFv3_RS03120 are separated by 23 nucleotides RALBFv3_RS03120 and RALBFv3_RS03125 are separated by 56 nucleotides
RALBFv3_RS03110: RALBFv3_RS03110 - DUF924 domain-containing protein, at 680,177 to 680,755
_RS03110
RALBFv3_RS03115: RALBFv3_RS03115 - oxygen-binding heme protein, at 680,800 to 681,204
_RS03115
RALBFv3_RS03120: RALBFv3_RS03120 - ABC transporter permease, at 681,228 to 683,759
_RS03120
RALBFv3_RS03125: RALBFv3_RS03125 - hypothetical protein, at 683,816 to 684,916
_RS03125
Position (kb) 681
682
683
684 Strain fitness (log2 ratio) -1
0
1 at 680.404 kb on - strand, within RALBFv3_RS03110 at 680.993 kb on + strand, within RALBFv3_RS03115 at 680.994 kb on - strand, within RALBFv3_RS03115 at 681.116 kb on + strand, within RALBFv3_RS03115 at 681.116 kb on + strand, within RALBFv3_RS03115 at 681.204 kb on + strand at 681.311 kb on + strand at 681.458 kb on - strand at 681.742 kb on + strand, within RALBFv3_RS03120 at 681.742 kb on + strand, within RALBFv3_RS03120 at 681.742 kb on + strand, within RALBFv3_RS03120 at 681.742 kb on + strand, within RALBFv3_RS03120 at 681.743 kb on - strand, within RALBFv3_RS03120 at 681.997 kb on + strand, within RALBFv3_RS03120 at 681.997 kb on + strand, within RALBFv3_RS03120 at 681.998 kb on - strand, within RALBFv3_RS03120 at 681.998 kb on - strand, within RALBFv3_RS03120 at 682.532 kb on - strand, within RALBFv3_RS03120 at 682.747 kb on + strand, within RALBFv3_RS03120 at 682.748 kb on - strand, within RALBFv3_RS03120 at 682.869 kb on - strand, within RALBFv3_RS03120 at 684.007 kb on - strand, within RALBFv3_RS03125 at 684.007 kb on - strand, within RALBFv3_RS03125 at 684.009 kb on + strand, within RALBFv3_RS03125 at 684.010 kb on - strand, within RALBFv3_RS03125 at 684.010 kb on - strand, within RALBFv3_RS03125 at 684.010 kb on - strand, within RALBFv3_RS03125 at 684.010 kb on - strand, within RALBFv3_RS03125 at 684.404 kb on + strand, within RALBFv3_RS03125
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol remove 680,404 - RALBFv3_RS03110 0.39 -1.3 680,993 + RALBFv3_RS03115 0.48 -1.0 680,994 - RALBFv3_RS03115 0.48 +1.0 681,116 + RALBFv3_RS03115 0.78 +0.2 681,116 + RALBFv3_RS03115 0.78 -0.5 681,204 + -0.1 681,311 + -1.0 681,458 - -0.9 681,742 + RALBFv3_RS03120 0.20 -0.0 681,742 + RALBFv3_RS03120 0.20 -0.3 681,742 + RALBFv3_RS03120 0.20 -0.3 681,742 + RALBFv3_RS03120 0.20 -0.5 681,743 - RALBFv3_RS03120 0.20 -1.1 681,997 + RALBFv3_RS03120 0.30 +0.3 681,997 + RALBFv3_RS03120 0.30 -0.0 681,998 - RALBFv3_RS03120 0.30 -0.1 681,998 - RALBFv3_RS03120 0.30 +0.4 682,532 - RALBFv3_RS03120 0.52 -0.2 682,747 + RALBFv3_RS03120 0.60 -0.8 682,748 - RALBFv3_RS03120 0.60 +0.2 682,869 - RALBFv3_RS03120 0.65 -0.1 684,007 - RALBFv3_RS03125 0.17 -0.7 684,007 - RALBFv3_RS03125 0.17 -1.0 684,009 + RALBFv3_RS03125 0.18 -0.5 684,010 - RALBFv3_RS03125 0.18 -0.0 684,010 - RALBFv3_RS03125 0.18 +0.3 684,010 - RALBFv3_RS03125 0.18 -0.3 684,010 - RALBFv3_RS03125 0.18 +0.0 684,404 + RALBFv3_RS03125 0.53 -0.3
Or see this region's nucleotide sequence