Experiment: SMSA with Ralstonia_antibiotic_mix_minus_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS12125 and RALBFv3_RS12130 are separated by 18 nucleotides RALBFv3_RS12130 and RALBFv3_RS12135 overlap by 8 nucleotides RALBFv3_RS12135 and RALBFv3_RS12140 are separated by 39 nucleotides RALBFv3_RS12140 and RALBFv3_RS12145 are separated by 39 nucleotides
RALBFv3_RS12125: RALBFv3_RS12125 - peptidase M48, at 2,673,828 to 2,675,534
_RS12125
RALBFv3_RS12130: RALBFv3_RS12130 - DUF2946 domain-containing protein, at 2,675,553 to 2,676,140
_RS12130
RALBFv3_RS12135: RALBFv3_RS12135 - alpha/beta hydrolase, at 2,676,133 to 2,677,257
_RS12135
RALBFv3_RS12140: RALBFv3_RS12140 - hypothetical protein, at 2,677,297 to 2,677,737
_RS12140
RALBFv3_RS12145: RALBFv3_RS12145 - lipopolysaccharide heptosyltransferase II, at 2,677,777 to 2,678,802
_RS12145
Position (kb)
2676
2677
2678 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2675.205 kb on + strand, within RALBFv3_RS12125 at 2675.205 kb on + strand, within RALBFv3_RS12125 at 2675.205 kb on + strand, within RALBFv3_RS12125 at 2675.205 kb on + strand, within RALBFv3_RS12125 at 2675.206 kb on - strand, within RALBFv3_RS12125 at 2675.206 kb on - strand, within RALBFv3_RS12125 at 2675.206 kb on - strand, within RALBFv3_RS12125 at 2675.206 kb on - strand, within RALBFv3_RS12125 at 2675.206 kb on - strand, within RALBFv3_RS12125 at 2675.206 kb on - strand, within RALBFv3_RS12125 at 2675.206 kb on - strand, within RALBFv3_RS12125 at 2675.253 kb on + strand, within RALBFv3_RS12125 at 2675.254 kb on - strand, within RALBFv3_RS12125 at 2675.319 kb on + strand, within RALBFv3_RS12125 at 2675.319 kb on + strand, within RALBFv3_RS12125 at 2675.319 kb on + strand, within RALBFv3_RS12125 at 2675.320 kb on - strand, within RALBFv3_RS12125 at 2675.370 kb on + strand at 2675.371 kb on - strand at 2675.371 kb on - strand at 2675.532 kb on + strand at 2675.533 kb on - strand at 2675.533 kb on - strand at 2675.533 kb on - strand at 2675.877 kb on + strand, within RALBFv3_RS12130 at 2675.877 kb on + strand, within RALBFv3_RS12130 at 2675.893 kb on + strand, within RALBFv3_RS12130 at 2675.894 kb on - strand, within RALBFv3_RS12130 at 2675.962 kb on + strand, within RALBFv3_RS12130 at 2675.962 kb on + strand, within RALBFv3_RS12130 at 2675.963 kb on - strand, within RALBFv3_RS12130 at 2675.963 kb on - strand, within RALBFv3_RS12130 at 2675.963 kb on - strand, within RALBFv3_RS12130 at 2676.034 kb on + strand, within RALBFv3_RS12130 at 2676.461 kb on + strand, within RALBFv3_RS12135 at 2676.467 kb on + strand, within RALBFv3_RS12135 at 2676.467 kb on + strand, within RALBFv3_RS12135 at 2676.467 kb on + strand, within RALBFv3_RS12135 at 2676.467 kb on + strand, within RALBFv3_RS12135 at 2676.468 kb on - strand, within RALBFv3_RS12135 at 2676.468 kb on - strand, within RALBFv3_RS12135 at 2676.468 kb on - strand, within RALBFv3_RS12135 at 2676.554 kb on + strand, within RALBFv3_RS12135 at 2676.555 kb on - strand, within RALBFv3_RS12135 at 2676.555 kb on - strand, within RALBFv3_RS12135 at 2676.623 kb on + strand, within RALBFv3_RS12135 at 2676.623 kb on + strand, within RALBFv3_RS12135 at 2676.623 kb on + strand, within RALBFv3_RS12135 at 2676.624 kb on - strand, within RALBFv3_RS12135 at 2676.624 kb on - strand, within RALBFv3_RS12135 at 2676.737 kb on + strand, within RALBFv3_RS12135 at 2676.863 kb on + strand, within RALBFv3_RS12135 at 2676.863 kb on + strand, within RALBFv3_RS12135 at 2676.863 kb on + strand, within RALBFv3_RS12135 at 2676.863 kb on + strand, within RALBFv3_RS12135 at 2676.863 kb on + strand, within RALBFv3_RS12135 at 2676.863 kb on + strand, within RALBFv3_RS12135 at 2676.863 kb on + strand, within RALBFv3_RS12135 at 2676.863 kb on + strand, within RALBFv3_RS12135 at 2676.864 kb on - strand, within RALBFv3_RS12135 at 2676.864 kb on - strand, within RALBFv3_RS12135 at 2676.864 kb on - strand, within RALBFv3_RS12135 at 2676.966 kb on - strand, within RALBFv3_RS12135 at 2677.409 kb on + strand, within RALBFv3_RS12140 at 2677.409 kb on + strand, within RALBFv3_RS12140 at 2677.409 kb on + strand, within RALBFv3_RS12140 at 2677.409 kb on + strand, within RALBFv3_RS12140 at 2677.409 kb on + strand, within RALBFv3_RS12140 at 2677.409 kb on + strand, within RALBFv3_RS12140 at 2677.409 kb on + strand, within RALBFv3_RS12140 at 2677.409 kb on + strand, within RALBFv3_RS12140 at 2677.409 kb on + strand, within RALBFv3_RS12140 at 2677.410 kb on - strand, within RALBFv3_RS12140 at 2677.410 kb on - strand, within RALBFv3_RS12140 at 2677.410 kb on - strand, within RALBFv3_RS12140 at 2677.424 kb on + strand, within RALBFv3_RS12140 at 2677.425 kb on - strand, within RALBFv3_RS12140 at 2677.425 kb on - strand, within RALBFv3_RS12140 at 2677.425 kb on - strand, within RALBFv3_RS12140 at 2677.425 kb on - strand, within RALBFv3_RS12140 at 2677.686 kb on - strand, within RALBFv3_RS12140 at 2677.778 kb on + strand at 2677.778 kb on + strand at 2677.908 kb on + strand, within RALBFv3_RS12145 at 2677.909 kb on - strand, within RALBFv3_RS12145 at 2677.909 kb on - strand, within RALBFv3_RS12145 at 2677.909 kb on - strand, within RALBFv3_RS12145 at 2678.237 kb on + strand, within RALBFv3_RS12145 at 2678.238 kb on - strand, within RALBFv3_RS12145 at 2678.238 kb on - strand, within RALBFv3_RS12145
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_CrystalViolet remove 2,675,205 + RALBFv3_RS12125 0.81 -0.3 2,675,205 + RALBFv3_RS12125 0.81 +1.0 2,675,205 + RALBFv3_RS12125 0.81 -0.3 2,675,205 + RALBFv3_RS12125 0.81 +0.1 2,675,206 - RALBFv3_RS12125 0.81 -0.9 2,675,206 - RALBFv3_RS12125 0.81 +1.0 2,675,206 - RALBFv3_RS12125 0.81 -0.2 2,675,206 - RALBFv3_RS12125 0.81 +0.1 2,675,206 - RALBFv3_RS12125 0.81 -0.2 2,675,206 - RALBFv3_RS12125 0.81 -0.2 2,675,206 - RALBFv3_RS12125 0.81 +0.0 2,675,253 + RALBFv3_RS12125 0.83 +0.2 2,675,254 - RALBFv3_RS12125 0.84 -0.7 2,675,319 + RALBFv3_RS12125 0.87 +0.2 2,675,319 + RALBFv3_RS12125 0.87 -0.1 2,675,319 + RALBFv3_RS12125 0.87 +0.0 2,675,320 - RALBFv3_RS12125 0.87 -0.2 2,675,370 + -0.2 2,675,371 - -0.4 2,675,371 - -0.2 2,675,532 + -0.4 2,675,533 - +0.6 2,675,533 - -0.5 2,675,533 - +1.2 2,675,877 + RALBFv3_RS12130 0.55 -0.4 2,675,877 + RALBFv3_RS12130 0.55 -0.1 2,675,893 + RALBFv3_RS12130 0.58 -0.2 2,675,894 - RALBFv3_RS12130 0.58 -0.4 2,675,962 + RALBFv3_RS12130 0.70 -0.8 2,675,962 + RALBFv3_RS12130 0.70 +0.0 2,675,963 - RALBFv3_RS12130 0.70 +0.9 2,675,963 - RALBFv3_RS12130 0.70 +0.5 2,675,963 - RALBFv3_RS12130 0.70 -0.3 2,676,034 + RALBFv3_RS12130 0.82 -0.0 2,676,461 + RALBFv3_RS12135 0.29 -0.0 2,676,467 + RALBFv3_RS12135 0.30 +0.4 2,676,467 + RALBFv3_RS12135 0.30 -0.7 2,676,467 + RALBFv3_RS12135 0.30 +1.0 2,676,467 + RALBFv3_RS12135 0.30 -2.4 2,676,468 - RALBFv3_RS12135 0.30 -0.3 2,676,468 - RALBFv3_RS12135 0.30 -0.8 2,676,468 - RALBFv3_RS12135 0.30 -0.8 2,676,554 + RALBFv3_RS12135 0.37 +0.3 2,676,555 - RALBFv3_RS12135 0.38 -0.2 2,676,555 - RALBFv3_RS12135 0.38 -1.2 2,676,623 + RALBFv3_RS12135 0.44 +0.1 2,676,623 + RALBFv3_RS12135 0.44 -0.2 2,676,623 + RALBFv3_RS12135 0.44 -0.1 2,676,624 - RALBFv3_RS12135 0.44 +0.4 2,676,624 - RALBFv3_RS12135 0.44 +0.0 2,676,737 + RALBFv3_RS12135 0.54 -0.0 2,676,863 + RALBFv3_RS12135 0.65 -0.6 2,676,863 + RALBFv3_RS12135 0.65 +0.1 2,676,863 + RALBFv3_RS12135 0.65 -0.5 2,676,863 + RALBFv3_RS12135 0.65 -0.5 2,676,863 + RALBFv3_RS12135 0.65 -0.0 2,676,863 + RALBFv3_RS12135 0.65 -1.2 2,676,863 + RALBFv3_RS12135 0.65 -1.1 2,676,863 + RALBFv3_RS12135 0.65 -0.1 2,676,864 - RALBFv3_RS12135 0.65 +1.0 2,676,864 - RALBFv3_RS12135 0.65 +0.4 2,676,864 - RALBFv3_RS12135 0.65 -0.5 2,676,966 - RALBFv3_RS12135 0.74 +0.2 2,677,409 + RALBFv3_RS12140 0.25 -0.6 2,677,409 + RALBFv3_RS12140 0.25 -0.4 2,677,409 + RALBFv3_RS12140 0.25 +1.0 2,677,409 + RALBFv3_RS12140 0.25 -0.4 2,677,409 + RALBFv3_RS12140 0.25 -0.8 2,677,409 + RALBFv3_RS12140 0.25 +0.4 2,677,409 + RALBFv3_RS12140 0.25 +1.0 2,677,409 + RALBFv3_RS12140 0.25 +0.6 2,677,409 + RALBFv3_RS12140 0.25 +0.4 2,677,410 - RALBFv3_RS12140 0.26 +0.3 2,677,410 - RALBFv3_RS12140 0.26 +0.4 2,677,410 - RALBFv3_RS12140 0.26 +0.2 2,677,424 + RALBFv3_RS12140 0.29 -0.7 2,677,425 - RALBFv3_RS12140 0.29 +0.5 2,677,425 - RALBFv3_RS12140 0.29 -0.1 2,677,425 - RALBFv3_RS12140 0.29 +0.4 2,677,425 - RALBFv3_RS12140 0.29 +0.4 2,677,686 - RALBFv3_RS12140 0.88 +0.5 2,677,778 + -0.3 2,677,778 + -0.2 2,677,908 + RALBFv3_RS12145 0.13 -1.3 2,677,909 - RALBFv3_RS12145 0.13 -0.0 2,677,909 - RALBFv3_RS12145 0.13 -0.6 2,677,909 - RALBFv3_RS12145 0.13 -1.6 2,678,237 + RALBFv3_RS12145 0.45 +1.0 2,678,238 - RALBFv3_RS12145 0.45 -1.3 2,678,238 - RALBFv3_RS12145 0.45 -2.8
Or see this region's nucleotide sequence