Strain Fitness in Ralstonia solanacearum IBSBF1503 around RALBFv3_RS10865

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_CrystalViolet

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRALBFv3_RS10860 and RALBFv3_RS10865 are separated by 108 nucleotidesRALBFv3_RS10865 and RALBFv3_RS10870 overlap by 32 nucleotidesRALBFv3_RS10870 and RALBFv3_RS10875 are separated by 40 nucleotides RALBFv3_RS10860: RALBFv3_RS10860 - glutathione synthase, at 2,406,144 to 2,407,118 _RS10860 RALBFv3_RS10865: RALBFv3_RS10865 - PTS fructose transporter subunit IIA, at 2,407,227 to 2,407,682 _RS10865 RALBFv3_RS10870: RALBFv3_RS10870 - HPr family phosphocarrier protein, at 2,407,651 to 2,407,920 _RS10870 RALBFv3_RS10875: RALBFv3_RS10875 - phosphoenolpyruvate--protein phosphotransferase, at 2,407,961 to 2,409,721 _RS10875 Position (kb) 2407 2408Strain fitness (log2 ratio) -2 -1 0 1at 2406.246 kb on + strand, within RALBFv3_RS10860at 2406.247 kb on - strand, within RALBFv3_RS10860at 2406.739 kb on - strand, within RALBFv3_RS10860at 2406.739 kb on - strand, within RALBFv3_RS10860at 2406.972 kb on + strand, within RALBFv3_RS10860at 2407.116 kb on + strandat 2407.193 kb on + strandat 2407.194 kb on - strandat 2407.534 kb on - strand, within RALBFv3_RS10865at 2407.594 kb on + strand, within RALBFv3_RS10865at 2407.595 kb on - strand, within RALBFv3_RS10865at 2407.650 kb on + strandat 2407.651 kb on - strandat 2407.651 kb on - strandat 2407.651 kb on - strandat 2407.651 kb on - strandat 2407.680 kb on + strandat 2407.680 kb on + strandat 2408.020 kb on + strandat 2408.054 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_CrystalViolet
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2,406,246 + RALBFv3_RS10860 0.10 -1.0
2,406,247 - RALBFv3_RS10860 0.11 +0.0
2,406,739 - RALBFv3_RS10860 0.61 -0.6
2,406,739 - RALBFv3_RS10860 0.61 -0.5
2,406,972 + RALBFv3_RS10860 0.85 -0.1
2,407,116 + -0.1
2,407,193 + -0.4
2,407,194 - -0.0
2,407,534 - RALBFv3_RS10865 0.67 +1.0
2,407,594 + RALBFv3_RS10865 0.80 -0.5
2,407,595 - RALBFv3_RS10865 0.81 +0.8
2,407,650 + +0.4
2,407,651 - -1.0
2,407,651 - -0.7
2,407,651 - -2.0
2,407,651 - -0.3
2,407,680 + -0.7
2,407,680 + -0.4
2,408,020 + +0.0
2,408,054 + -2.2

Or see this region's nucleotide sequence