Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Penicillin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS23935 and RALBFv3_RS11040 overlap by 14 nucleotides RALBFv3_RS11040 and RALBFv3_RS11045 are separated by 150 nucleotides RALBFv3_RS11045 and RALBFv3_RS11050 are separated by 3 nucleotides RALBFv3_RS11050 and RALBFv3_RS11055 are separated by 205 nucleotides
RALBFv3_RS23935: RALBFv3_RS23935 - hypothetical protein, at 2,439,123 to 2,439,402
_RS23935
RALBFv3_RS11040: RALBFv3_RS11040 - response regulator, at 2,439,389 to 2,440,015
_RS11040
RALBFv3_RS11045: RALBFv3_RS11045 - FAA hydrolase family protein, at 2,440,166 to 2,440,867
_RS11045
RALBFv3_RS11050: RALBFv3_RS11050 - maleylacetoacetate isomerase, at 2,440,871 to 2,441,521
_RS11050
RALBFv3_RS11055: RALBFv3_RS11055 - signal recognition particle-docking protein FtsY, at 2,441,727 to 2,442,860
_RS11055
Position (kb)
2440
2441 Strain fitness (log2 ratio)
-2
-1
0
1 at 2439.661 kb on + strand, within RALBFv3_RS11040 at 2439.661 kb on + strand, within RALBFv3_RS11040 at 2439.662 kb on - strand, within RALBFv3_RS11040 at 2439.663 kb on + strand, within RALBFv3_RS11040 at 2440.099 kb on + strand at 2440.099 kb on + strand at 2440.099 kb on + strand at 2440.100 kb on - strand at 2440.412 kb on + strand, within RALBFv3_RS11045 at 2440.483 kb on + strand, within RALBFv3_RS11045 at 2440.483 kb on + strand, within RALBFv3_RS11045 at 2440.483 kb on + strand, within RALBFv3_RS11045 at 2440.484 kb on - strand, within RALBFv3_RS11045 at 2440.487 kb on + strand, within RALBFv3_RS11045 at 2440.487 kb on + strand, within RALBFv3_RS11045 at 2440.488 kb on - strand, within RALBFv3_RS11045 at 2440.493 kb on + strand, within RALBFv3_RS11045 at 2440.887 kb on - strand at 2440.892 kb on + strand at 2440.893 kb on - strand at 2441.088 kb on - strand, within RALBFv3_RS11050 at 2441.216 kb on + strand, within RALBFv3_RS11050 at 2441.426 kb on + strand, within RALBFv3_RS11050
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Penicillin remove 2,439,661 + RALBFv3_RS11040 0.43 -0.2 2,439,661 + RALBFv3_RS11040 0.43 -0.1 2,439,662 - RALBFv3_RS11040 0.44 +0.5 2,439,663 + RALBFv3_RS11040 0.44 +0.2 2,440,099 + -0.3 2,440,099 + +0.0 2,440,099 + -0.0 2,440,100 - -0.2 2,440,412 + RALBFv3_RS11045 0.35 +0.1 2,440,483 + RALBFv3_RS11045 0.45 -0.2 2,440,483 + RALBFv3_RS11045 0.45 -0.3 2,440,483 + RALBFv3_RS11045 0.45 +0.0 2,440,484 - RALBFv3_RS11045 0.45 -0.3 2,440,487 + RALBFv3_RS11045 0.46 +0.8 2,440,487 + RALBFv3_RS11045 0.46 +1.1 2,440,488 - RALBFv3_RS11045 0.46 -1.3 2,440,493 + RALBFv3_RS11045 0.47 -0.2 2,440,887 - -0.7 2,440,892 + -0.8 2,440,893 - -2.4 2,441,088 - RALBFv3_RS11050 0.33 -0.9 2,441,216 + RALBFv3_RS11050 0.53 +0.3 2,441,426 + RALBFv3_RS11050 0.85 -0.5
Or see this region's nucleotide sequence