Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Penicillin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS05045 and RALBFv3_RS05050 are separated by 282 nucleotides RALBFv3_RS05050 and RALBFv3_RS05055 are separated by 93 nucleotides RALBFv3_RS05055 and RALBFv3_RS05060 are separated by 146 nucleotides
RALBFv3_RS05045: RALBFv3_RS05045 - site-2 protease family protein, at 1,122,747 to 1,123,472
_RS05045
RALBFv3_RS05050: RALBFv3_RS05050 - beta-ketoacyl-ACP reductase, at 1,123,755 to 1,124,507
_RS05050
RALBFv3_RS05055: RALBFv3_RS05055 - superoxide dismutase, at 1,124,601 to 1,125,179
_RS05055
RALBFv3_RS05060: RALBFv3_RS05060 - exodeoxyribonuclease VII large subunit, at 1,125,326 to 1,126,732
_RS05060
Position (kb)
1123
1124
1125 Strain fitness (log2 ratio)
-1
0
1
2 at 1122.909 kb on + strand, within RALBFv3_RS05045 at 1122.909 kb on + strand, within RALBFv3_RS05045 at 1122.910 kb on - strand, within RALBFv3_RS05045 at 1123.072 kb on - strand, within RALBFv3_RS05045 at 1123.119 kb on + strand, within RALBFv3_RS05045 at 1123.119 kb on + strand, within RALBFv3_RS05045 at 1123.120 kb on - strand, within RALBFv3_RS05045 at 1123.317 kb on + strand, within RALBFv3_RS05045 at 1123.318 kb on - strand, within RALBFv3_RS05045 at 1123.350 kb on + strand, within RALBFv3_RS05045 at 1123.350 kb on + strand, within RALBFv3_RS05045 at 1123.351 kb on - strand, within RALBFv3_RS05045 at 1123.389 kb on + strand, within RALBFv3_RS05045 at 1123.389 kb on + strand, within RALBFv3_RS05045 at 1123.389 kb on + strand, within RALBFv3_RS05045 at 1123.389 kb on + strand, within RALBFv3_RS05045 at 1123.389 kb on + strand, within RALBFv3_RS05045 at 1123.390 kb on - strand, within RALBFv3_RS05045 at 1123.390 kb on - strand, within RALBFv3_RS05045 at 1123.392 kb on + strand, within RALBFv3_RS05045 at 1123.393 kb on - strand, within RALBFv3_RS05045 at 1123.428 kb on + strand at 1123.428 kb on + strand at 1123.429 kb on - strand at 1123.429 kb on - strand at 1123.429 kb on - strand at 1123.444 kb on + strand at 1123.444 kb on + strand at 1123.444 kb on + strand at 1123.445 kb on - strand at 1123.445 kb on - strand at 1123.445 kb on - strand at 1123.607 kb on + strand at 1123.608 kb on - strand at 1123.608 kb on - strand at 1124.134 kb on - strand, within RALBFv3_RS05050 at 1124.220 kb on + strand, within RALBFv3_RS05050 at 1124.220 kb on + strand, within RALBFv3_RS05050 at 1124.220 kb on + strand, within RALBFv3_RS05050 at 1124.221 kb on - strand, within RALBFv3_RS05050 at 1124.221 kb on - strand, within RALBFv3_RS05050 at 1124.221 kb on - strand, within RALBFv3_RS05050 at 1124.221 kb on - strand, within RALBFv3_RS05050 at 1124.221 kb on - strand, within RALBFv3_RS05050 at 1124.316 kb on + strand, within RALBFv3_RS05050 at 1124.316 kb on + strand, within RALBFv3_RS05050 at 1124.316 kb on + strand, within RALBFv3_RS05050 at 1124.498 kb on + strand at 1124.498 kb on + strand at 1124.499 kb on - strand at 1124.499 kb on - strand at 1124.505 kb on + strand at 1124.505 kb on + strand at 1124.506 kb on - strand at 1124.506 kb on - strand at 1125.225 kb on + strand at 1125.226 kb on - strand at 1125.331 kb on + strand at 1125.332 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Penicillin remove 1,122,909 + RALBFv3_RS05045 0.22 +2.1 1,122,909 + RALBFv3_RS05045 0.22 +0.1 1,122,910 - RALBFv3_RS05045 0.22 +0.3 1,123,072 - RALBFv3_RS05045 0.45 -0.6 1,123,119 + RALBFv3_RS05045 0.51 +0.1 1,123,119 + RALBFv3_RS05045 0.51 +0.1 1,123,120 - RALBFv3_RS05045 0.51 -0.4 1,123,317 + RALBFv3_RS05045 0.79 -0.9 1,123,318 - RALBFv3_RS05045 0.79 +0.7 1,123,350 + RALBFv3_RS05045 0.83 -0.5 1,123,350 + RALBFv3_RS05045 0.83 +2.1 1,123,351 - RALBFv3_RS05045 0.83 +1.1 1,123,389 + RALBFv3_RS05045 0.88 +1.5 1,123,389 + RALBFv3_RS05045 0.88 -0.2 1,123,389 + RALBFv3_RS05045 0.88 -0.7 1,123,389 + RALBFv3_RS05045 0.88 +0.1 1,123,389 + RALBFv3_RS05045 0.88 -0.4 1,123,390 - RALBFv3_RS05045 0.89 +0.2 1,123,390 - RALBFv3_RS05045 0.89 -0.2 1,123,392 + RALBFv3_RS05045 0.89 -1.5 1,123,393 - RALBFv3_RS05045 0.89 -0.4 1,123,428 + +0.5 1,123,428 + -1.1 1,123,429 - +0.1 1,123,429 - -0.3 1,123,429 - -0.5 1,123,444 + +0.1 1,123,444 + -0.9 1,123,444 + -0.1 1,123,445 - +1.1 1,123,445 - -0.2 1,123,445 - -0.5 1,123,607 + -0.5 1,123,608 - +0.1 1,123,608 - -0.5 1,124,134 - RALBFv3_RS05050 0.50 +0.4 1,124,220 + RALBFv3_RS05050 0.62 +0.1 1,124,220 + RALBFv3_RS05050 0.62 -0.4 1,124,220 + RALBFv3_RS05050 0.62 -0.9 1,124,221 - RALBFv3_RS05050 0.62 -0.9 1,124,221 - RALBFv3_RS05050 0.62 -0.2 1,124,221 - RALBFv3_RS05050 0.62 -0.1 1,124,221 - RALBFv3_RS05050 0.62 +0.4 1,124,221 - RALBFv3_RS05050 0.62 -0.7 1,124,316 + RALBFv3_RS05050 0.75 +1.1 1,124,316 + RALBFv3_RS05050 0.75 +1.1 1,124,316 + RALBFv3_RS05050 0.75 -0.8 1,124,498 + -0.2 1,124,498 + +0.1 1,124,499 - +0.1 1,124,499 - +0.5 1,124,505 + +0.1 1,124,505 + +1.7 1,124,506 - -0.5 1,124,506 - +0.0 1,125,225 + +0.5 1,125,226 - +0.5 1,125,331 + -0.9 1,125,332 - -0.9
Or see this region's nucleotide sequence