Strain Fitness in Ralstonia solanacearum IBSBF1503 around RALBFv3_RS01705

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRALBFv3_RS01695 and RALBFv3_RS01700 are separated by 149 nucleotidesRALBFv3_RS01700 and RALBFv3_RS01705 are separated by 14 nucleotidesRALBFv3_RS01705 and RALBFv3_RS01710 are separated by 10 nucleotides RALBFv3_RS01695: RALBFv3_RS01695 - cytosine deaminase, at 383,189 to 384,460 _RS01695 RALBFv3_RS01700: RALBFv3_RS01700 - exodeoxyribonuclease III, at 384,610 to 385,419 _RS01700 RALBFv3_RS01705: RALBFv3_RS01705 - homoprotocatechuate degradation operon regulator HpaR, at 385,434 to 385,898 _RS01705 RALBFv3_RS01710: RALBFv3_RS01710 - 4-hydroxy-2-oxoheptanedioate aldolase, at 385,909 to 386,715 _RS01710 Position (kb) 385 386Strain fitness (log2 ratio) -1 0 1at 384.543 kb on + strandat 384.809 kb on - strand, within RALBFv3_RS01700at 385.001 kb on - strand, within RALBFv3_RS01700at 385.824 kb on + strand, within RALBFv3_RS01705at 385.913 kb on + strandat 386.042 kb on + strand, within RALBFv3_RS01710at 386.042 kb on + strand, within RALBFv3_RS01710at 386.043 kb on - strand, within RALBFv3_RS01710at 386.043 kb on - strand, within RALBFv3_RS01710at 386.306 kb on + strand, within RALBFv3_RS01710at 386.307 kb on - strand, within RALBFv3_RS01710at 386.808 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol
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384,543 + -0.0
384,809 - RALBFv3_RS01700 0.25 -1.7
385,001 - RALBFv3_RS01700 0.48 -1.2
385,824 + RALBFv3_RS01705 0.84 -0.6
385,913 + +0.3
386,042 + RALBFv3_RS01710 0.16 -0.6
386,042 + RALBFv3_RS01710 0.16 +1.0
386,043 - RALBFv3_RS01710 0.17 -0.5
386,043 - RALBFv3_RS01710 0.17 +0.7
386,306 + RALBFv3_RS01710 0.49 -0.0
386,307 - RALBFv3_RS01710 0.49 -0.0
386,808 - -0.8

Or see this region's nucleotide sequence