Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Bacitracin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS02260 and RALBFv3_RS02265 are separated by 486 nucleotides RALBFv3_RS02265 and RALBFv3_RS02270 are separated by 9 nucleotides RALBFv3_RS02270 and RALBFv3_RS02275 are separated by 27 nucleotides
RALBFv3_RS02260: RALBFv3_RS02260 - universal stress protein, at 502,937 to 503,392
_RS02260
RALBFv3_RS02265: RALBFv3_RS02265 - SCO family protein, at 503,879 to 505,525
_RS02265
RALBFv3_RS02270: RALBFv3_RS02270 - hypothetical protein, at 505,535 to 506,476
_RS02270
RALBFv3_RS02275: RALBFv3_RS02275 - tyrosinase, at 506,504 to 507,742
_RS02275
Position (kb) 503
504
505
506 Strain fitness (log2 ratio) -2
-1
0
1
2 at 502.881 kb on - strand at 502.940 kb on + strand at 502.940 kb on + strand at 502.940 kb on + strand at 503.371 kb on - strand at 503.762 kb on + strand at 503.880 kb on + strand at 503.880 kb on + strand at 504.134 kb on + strand, within RALBFv3_RS02265 at 504.134 kb on + strand, within RALBFv3_RS02265 at 504.234 kb on + strand, within RALBFv3_RS02265 at 504.235 kb on - strand, within RALBFv3_RS02265 at 504.235 kb on - strand, within RALBFv3_RS02265 at 504.267 kb on + strand, within RALBFv3_RS02265 at 504.622 kb on - strand, within RALBFv3_RS02265 at 504.660 kb on + strand, within RALBFv3_RS02265 at 504.660 kb on + strand, within RALBFv3_RS02265 at 504.661 kb on - strand, within RALBFv3_RS02265 at 504.778 kb on - strand, within RALBFv3_RS02265 at 504.778 kb on - strand, within RALBFv3_RS02265 at 504.778 kb on - strand, within RALBFv3_RS02265 at 505.267 kb on - strand, within RALBFv3_RS02265 at 505.267 kb on - strand, within RALBFv3_RS02265 at 505.396 kb on - strand at 505.687 kb on + strand, within RALBFv3_RS02270 at 505.863 kb on - strand, within RALBFv3_RS02270 at 505.930 kb on - strand, within RALBFv3_RS02270 at 505.950 kb on + strand, within RALBFv3_RS02270 at 505.950 kb on + strand, within RALBFv3_RS02270 at 505.951 kb on - strand, within RALBFv3_RS02270 at 505.951 kb on - strand, within RALBFv3_RS02270
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Bacitracin remove 502,881 - -0.1 502,940 + -0.2 502,940 + -0.8 502,940 + -0.8 503,371 - -0.4 503,762 + -0.6 503,880 + -0.3 503,880 + -0.4 504,134 + RALBFv3_RS02265 0.15 +0.4 504,134 + RALBFv3_RS02265 0.15 +2.1 504,234 + RALBFv3_RS02265 0.22 +0.7 504,235 - RALBFv3_RS02265 0.22 +0.4 504,235 - RALBFv3_RS02265 0.22 +0.3 504,267 + RALBFv3_RS02265 0.24 -0.3 504,622 - RALBFv3_RS02265 0.45 +1.4 504,660 + RALBFv3_RS02265 0.47 +0.0 504,660 + RALBFv3_RS02265 0.47 +0.3 504,661 - RALBFv3_RS02265 0.47 +0.3 504,778 - RALBFv3_RS02265 0.55 +0.7 504,778 - RALBFv3_RS02265 0.55 +0.0 504,778 - RALBFv3_RS02265 0.55 +0.2 505,267 - RALBFv3_RS02265 0.84 -0.4 505,267 - RALBFv3_RS02265 0.84 -0.0 505,396 - +0.1 505,687 + RALBFv3_RS02270 0.16 -0.1 505,863 - RALBFv3_RS02270 0.35 -0.7 505,930 - RALBFv3_RS02270 0.42 +0.2 505,950 + RALBFv3_RS02270 0.44 -0.1 505,950 + RALBFv3_RS02270 0.44 -2.1 505,951 - RALBFv3_RS02270 0.44 -0.1 505,951 - RALBFv3_RS02270 0.44 +0.5
Or see this region's nucleotide sequence